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Invisiblethirdeyewild
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28672827 - 02/23/24 01:15 PM (4 months, 13 hours ago)

It can't hurt to watch the plate to see if you get other germinations. But I don't see anything that could be cleaned up out of that spot.


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OfflineYahra
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Re: Ask Quick Questions, Get Quick Answers [Re: thirdeyewild]
    #28672832 - 02/23/24 01:18 PM (4 months, 13 hours ago)

The little white dots at 7 o'clock right beneath the yellow should be mycelium.

That's where i scraped the scalpel. You can see the cuts in the agar if you look closely.

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OfflineSkropi
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28672839 - 02/23/24 01:23 PM (4 months, 13 hours ago)

Quote:

Yahra said:
The little white dots at 7 o'clock right beneath the yellow should be mycelium.

That's where i scraped the scalpel. You can see the cuts in the agar if you look closely.



Could you take a better pic of those spots? I can't discern them easily, and I am curious. If this orange spot is away from the spot you inoculated, then you can definitely save the plate, as bacteria don't spread very quickly like mold. I thought that this spot was your inoculation point.


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Ἄνδρα μοι ἔννεπε, Μοῦσα, πολύτροπον

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Invisiblethirdeyewild
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Re: Ask Quick Questions, Get Quick Answers [Re: Skropi]
    #28672844 - 02/23/24 01:25 PM (4 months, 13 hours ago)



Edit. Right 7 o'clock. Yeah looks clean, I usually let things get a little bigger before transfer but I can see why you wanted to get it out of there as soon as possible.


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Edited by thirdeyewild (02/23/24 01:29 PM)

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Offlineoaklandish
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Re: Ask Quick Questions, Get Quick Answers [Re: Skropi]
    #28672849 - 02/23/24 01:28 PM (4 months, 13 hours ago)

It’s been a minute since I’ve grown anything on grain, and maybe this is good to go, but the way it’s looking seems off to me, maybe?

This is p. natalensis.  Liquid culture, tested twice on agar, looked good on agar. PCd grain jars for two hours, inoculated grain jars with 10ml of LC a week or so ago. They were showing nothing a day or two ago but they’re all showing now.

The “hairiness” of this seems off to me but maybe it’s nothing. If it’s of any relevance they’re in a 78 degree room.








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Offlinekoma23
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Re: Ask Quick Questions, Get Quick Answers [Re: oaklandish]
    #28672851 - 02/23/24 01:30 PM (4 months, 13 hours ago)

Does mycelium growing on agar benefit from a light source or is it just the same if you keep it in a closet?

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OfflineSmellyhobbitM
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Re: Ask Quick Questions, Get Quick Answers [Re: oaklandish]
    #28672852 - 02/23/24 01:31 PM (4 months, 13 hours ago)

Yahra did you streak it or just put a drop in the center?


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OfflineYahra
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28672857 - 02/23/24 01:40 PM (4 months, 13 hours ago)

I flamed my scalpel until red hot, cooled it down in the agar and scraped a few spores from the print onto the blade.

Then i rubbed each side of the blade against the agar.

Pretty much in the center. No fancy streaking in patterns involved.

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OfflineYahra
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Re: Ask Quick Questions, Get Quick Answers [Re: Skropi]
    #28672870 - 02/23/24 01:54 PM (4 months, 12 hours ago)

Can't take good pics, my camera doesn't focus properly.

But the more pics i take, the more i think that's not myc but a reflection of the camera light off the glass since i poured very thin.






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OfflineSkropi
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28672873 - 02/23/24 01:59 PM (4 months, 12 hours ago)

Quote:

Yahra said:
Can't take good pics, my camera doesn't focus properly.

But the more pics i take, the more i think that's not myc but a reflection of the camera light off the glass since i poured very thin.









Ok, now I see the spores. It doesn't look good, but I would wait, hoping to get at least some myc showing, before the bacteria takes over, and transfer from the best looking edge using a needle point. Frankly, I believe that you will manage to get a transfer out of this plate. Key word, "believe".


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Ἄνδρα μοι ἔννεπε, Μοῦσα, πολύτροπον

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Offlinefiddle_head
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Re: Ask Quick Questions, Get Quick Answers [Re: oaklandish]
    #28672878 - 02/23/24 02:02 PM (4 months, 12 hours ago)

Quote:

oaklandish said:
It’s been a minute since I’ve grown anything on grain, and maybe this is good to go, but the way it’s looking seems off to me, maybe?

This is p. natalensis.  Liquid culture, tested twice on agar, looked good on agar. PCd grain jars for two hours, inoculated grain jars with 10ml of LC a week or so ago. They were showing nothing a day or two ago but they’re all showing now.

The “hairiness” of this seems off to me but maybe it’s nothing. If it’s of any relevance they’re in a 78 degree room.





looks like mycelium to me but im no nat expert.


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lagm 2024 || lagm 2023 || 2023 || Things That Are Good
=============
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🌿 Looking for spores? Look no further. 🐒

XI 🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 XI

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InvisibleMr Piggy
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 4
    #28672913 - 02/23/24 02:42 PM (4 months, 12 hours ago)

Quote:

Yahra said:
This is my first plate ever and it has a special place in my heart.




I totally get that, first plate is special.

However, some of the best advice I can give about cult is be ruthless, absolutely cutthroat.  If a culture doesn't perform perfectly, axe that motherfucker.  jar look sus?  Chuck it.  Tub taking way too long?  Dump it and start over.

All that iffy stuff just takes up space, resources, and time that could be going to performing genetics and clean cultures.


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OfflineYahra
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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #28672914 - 02/23/24 02:45 PM (4 months, 12 hours ago)

Good advice, thanks!

:grampofapproval:

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OfflineSkropi
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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #28672950 - 02/23/24 03:20 PM (4 months, 11 hours ago)

Quote:

Mr Piggy said:
Quote:

Yahra said:
This is my first plate ever and it has a special place in my heart.




I totally get that, first plate is special.

However, some of the best advice I can give about cult is be ruthless, absolutely cutthroat.  If a culture doesn't perform perfectly, axe that motherfucker.  jar look sus?  Chuck it.  Tub taking way too long?  Dump it and start over.

All that iffy stuff just takes up space, resources, and time that could be going to performing genetics and clean cultures.



Yeah, I am just starting my second grow, and I've already came to realise how true your words ring. It is all about efficiency.


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InvisibleNichrome
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 4
    #28672996 - 02/23/24 04:20 PM (4 months, 10 hours ago)

Quote:

Yahra said:
Quote:

Yahra said:
One year old spores on BRF agar inoculated 10 days ago.

That's a toss, right?









Since no one answered, i rephrase my question.

Does it make sense to wait for mycelium showing up and try a transfer or is it futile since contam has a massive head start?



That looks like very slow moving/non motile bacteria (likely streptococcus from the look of it) and if those spores germinate the mycelium should be plenty easy to transfer cleanly in one to two moves if you do well.

I deal with nastier shit all the time when working with wild tissue/spores.

Just don't let any condensation liquid roll across that bac colony. Any liquid will transport them and help them proliferate.


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question everything, it's very responsive



“Drink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.”
― Thich Nhat Hanh


Gypsum

Edited by Nichrome (02/23/24 04:22 PM)

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OfflineYahra
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28673002 - 02/23/24 04:24 PM (4 months, 10 hours ago)

Thanks!

I guess i'll keep that plate around a little bit longer and see how things develop.

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OfflineStroms
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 1
    #28673004 - 02/23/24 04:24 PM (4 months, 10 hours ago)

Gamechanger for me taking pics of plates was to put it in manual mode and move your camera closer or further depending on manual zoom you have set. The idea is to move the camera so that it focuses on the agar surface not the plate. With auto focus it always focused on top of plate for me.

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Offlineoaklandish
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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head]
    #28673019 - 02/23/24 04:48 PM (4 months, 10 hours ago)

Quote:

fiddle_head said:
Quote:

oaklandish said:
It’s been a minute since I’ve grown anything on grain, and maybe this is good to go, but the way it’s looking seems off to me, maybe?

This is p. natalensis.  Liquid culture, tested twice on agar, looked good on agar. PCd grain jars for two hours, inoculated grain jars with 10ml of LC a week or so ago. They were showing nothing a day or two ago but they’re all showing now.

The “hairiness” of this seems off to me but maybe it’s nothing. If it’s of any relevance they’re in a 78 degree room.





looks like mycelium to me but im no nat expert.




I kinda figured it was fine.  Thanks for replying!

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InvisibleYuggoth
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 4
    #28673412 - 02/23/24 10:09 PM (4 months, 4 hours ago)

Quote:

Yahra said:
Thanks!

I guess i'll keep that plate around a little bit longer and see how things develop.




Trying to work with what you have is how you get the experience that makes you wisely ruthless like Mr Piggy. I would keep that plate and see what I could do with it -- while also accepting it may well be a dead end.

It works out or not, either way you learn something, and if it craps out you haven't lost much.


--------------------
We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

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InvisibleSWBZA
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28673888 - 02/24/24 09:27 AM (3 months, 30 days ago)

QQ: What is currently the best popcorn tek? Thanks in advance

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