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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28678158 -

Yahra said:
Spore print to BRF agar 14 days ago. No growth yet.



Did i mess up?


It can take alot longer than that for germination, whats your recipe, agara content?


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----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions, Get Quick Answers [Re: Bigworm] * 1
    #28678159 -

Bigworm said:
ibz said:
Bigworm said:
I'm not sure what the spots on the caps are. They had them from the start. I was thinking pseudo but no other signs of it and they aren't slimy. Any ideas?




P.E?


Machine elf


Strange...does look like pseudo...Can't say for 100%
Does look better than my Machine elf :frown:


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Edited by ibz (02/27/24 02:21 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head]
    #28678160 -

I've edited my post for the receipe.


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 1
    #28678166 -

maybe try going less than 2% agar? 4.5g is over 2% for 200ml. the softer agar might aid in germination.


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----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions, Get Quick Answers [Re: Bigworm] * 2
    #28678182 -

Bigworm said:
ibz said:
Bigworm said:
I'm not sure what the spots on the caps are. They had them from the start. I was thinking pseudo but no other signs of it and they aren't slimy. Any ideas?




P.E?


Machine elf


Pretty sure it’s just genetic. I have varieties that always do it and varieties that never do.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28678197 -

Smellyhobbit said:
Bigworm said:
ibz said:
Bigworm said:
I'm not sure what the spots on the caps are. They had them from the start. I was thinking pseudo but no other signs of it and they aren't slimy. Any ideas?




P.E?


Machine elf


Pretty sure it’s just genetic. I have varieties that always do it and varieties that never do.


That happens with the sprinkler drops, you should use a softer one. It happened to me several times and when I changed it for a milder one it was solved, if it is not solved it is of bacterial origin


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" Antes de que cambien los reinos, es necesario que cambien los individuos"

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Re: Ask Quick Questions, Get Quick Answers [Re: DRAKENCL]
    #28678229 -

I use a cosmetic mister, which is very gentle. And it happens to Kodama whether you spray it or not. It’s definitely not a sure sign of infection or problems since it just happens in healthy grows sometimes.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head]
    #28678245 -

fiddle_head said:
maybe try going less than 2% agar? 4.5g is over 2% for 200ml. the softer agar might aid in germination.


This is a good advice, thanks.

What would be a good percentage of agar to help with germination?

1%, 1.5%, other? Should i reduce the amount of BRF too?


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28678259 -

Yahra said:
fiddle_head said:
maybe try going less than 2% agar? 4.5g is over 2% for 200ml. the softer agar might aid in germination.


This is a good advice, thanks.

What would be a good percentage of agar to help with germination?

1%, 1.5%, other? Should i reduce the amount of BRF too?


I do 1-1.4% for water agar but here ya go


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head]
    #28678265 -

So water agar is used for germination of spores rather than nutritional agar?

Thought it was meant for cleaning up.


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 1
    #28678281 -

Sorry,  I’m sorta new here this is my very first attempt and first post. I’m 2 weeks in and using nothing but cooked brown rice and this jar. And today I just noticed this brownish blackish spot on my spawn. Right where this big black mass of clumped spores was. Is it just the spores spreading, or is it something else more? And if so what would be the best way to go about remedying this situation?


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WHY SO SERIOUS???

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Re: Ask Quick Questions, Get Quick Answers [Re: Jester36]
    #28678283 -

Welcome to the Shroomery.

That is most likely contamination, and there is nothing to be done for that jar, except to dump it.

There are many great write ups on this site. I have my favs inside the manual in my signature.
Good luck to you.
:cheers:


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The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

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Re: Ask Quick Questions, Get Quick Answers [Re: Jester36] * 1
    #28678330 -

Looks too wet to me. I would just toss it. Just read on some well established Teks mate.


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Ἄνδρα ΞΌΞΏΞΉ ἔννΡπΡ, ΞœΞΏαΏ¦ΟƒΞ±, πολύτροπον

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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28678336 -

Yahra said:
Spore print to BRF agar 14 days ago. No growth yet.



200ml water, 4.5 grams agar and 3 grams BRF.

Did i mess up?




You didn't necessarily mess up. Sometimes spores act difficult and don't want to germinate.

I haven't used BRF agar before, so I can't comment on that. But how did you transfer the spores? Did you use an inoculation loop, or a swab, or did you just drip some onto the plate?


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Important Links.

Be kind to others.

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Re: Ask Quick Questions, Get Quick Answers [Re: Jester36]
    #28678341 -

Jester36 said:
Sorry,  I’m sorta new here this is my very first attempt and first post. I’m 2 weeks in and using nothing but cooked brown rice and this jar. And today I just noticed this brownish blackish spot on my spawn. Right where this big black mass of clumped spores was. Is it just the spores spreading, or is it something else more? And if so what would be the best way to go about remedying this situation?



Unfortunately you'll need to dump that, it's contaminated. That chunky white stuff looks like yeast to me.


--------------------


Important Links.

Be kind to others.

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Re: Ask Quick Questions, Get Quick Answers [Re: joze]
    #28678359 -

I flamed my scalpel until red hot, cooled it down in the agar and then used the blade to scrape some spores off of the print.


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28678390 -

Yahra said:
I flamed my scalpel until red hot, cooled it down in the agar and then used the blade to scrape some spores off of the print.


Okay. So you picked up some spores on the blade of your scalpel, and then wiped the blade onto the agar? Or did you just let the spores fall onto the agar?

I ask, because anecdotally I find that I get much better germination when my spores get squished down into the agar. I suspect it allows them to absorb moisture more easily? But I always use a sterile cotton swab, grab some spores, and then zig-zag the swab across the agar. I find I get much much much better germination than when I used to use my scalpel, like you are doing.


--------------------


Important Links.

Be kind to others.

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Re: Ask Quick Questions, Get Quick Answers [Re: joze]
    #28678402 -

I wiped the blade onto the agar. With a bit of force, you even can see a cut in the photo.



I think i should try cotton swabs next time, this is a nice idea. Thanks!


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 2
    #28678413 -

Get an inoculation loop or sterilize* some swabs and streak. Some people get away with the scalpel swipe, but it’s not a good replacement for a loop or swab.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Edited by Smellyhobbit (02/27/24 06:32 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28678416 -

Yahra said:
I wiped the blade onto the agar. With a bit of force, you even can see a cut in the photo.



I think i should try cotton swabs next time, this is a nice idea. Thanks!


Yeah, I'd give it a shot. I'd again like to point out that this is anecdotal. But I do seem to get more consistent germination with the swabs. I also really like the convenience. Here's the TEK I use.


--------------------


Important Links.

Be kind to others.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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