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GenesisCorrupted
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28669813 - 02/21/24 01:56 PM (4 months, 1 day ago) |
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This seems like an interesting problem. I’m gonna just say some ideas and see what you think. Is it possible that when there is wind outside of your house there is some air getting blown in your house, causing a draft with contaminants in it.
Because if your air conditioner heater was on. Obviously, that could cause something. But only if your air filter is dirty. But I don’t see why you’d have that on to begin with, so I’m thinking there’s probably a draft somewhere in your house. Then you would have contaminants suspended in your air from gust of wind from an hour ago and you wouldn’t even know.
Because the level of sanitation that tapwater goes through is excessive in my opinion. There is lingering chlorine that needs to be dissipated for three days before I can use it to water my plants. I really doubt there is something wrong with your water unless the spigot that it’s coming out of has contamination in it that’s imparted into the water before it even comes out.
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mushman509
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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
#28669823 - 02/21/24 02:02 PM (4 months, 1 day ago) |
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It’s been 2 weeks since inoculation with my AIO grow bag. I used liquid culture. No signs of contamination but no signs of growth either. I injected just over 2 weeks ago. The middle is more firm than the rest but that could just be how the bag is? It could also mean growth is happening but only in the middle right now? This is my first time trying. Temps are 70-72 degrees but I'm getting a seedling mat to place some distance below it (to provide ambient heat). Any tips or help would be appreciated.
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Lemgrub



Registered: 04/02/18
Posts: 644
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Quote:
GenesisCorrupted said: Do you think he had a dirty tap? I would just say something happened in the house that you weren’t aware of like something fell over a cat or dog just went in there and scratch themselves. Maybe there was some hidden debris floating in the air air that you didn’t know about when you came into the room to do your mono.
Nah, none of that would contaminate a clean grow. The quality of your water will absolutely compromise the virility of your culture if it's bad. And there are tons of inhibiting vectors on grain.
I've experienced poor water first hand, as my last place had well water and the watershed we were in tested high in Pb, As, and one or two other compounds.
I found through some research some metals and VOCs inhibit fruiting body initiation and growth in oysters significantly. Obviously you can't extrapolate much across species, but without research on cubes directly that's all I had to go by. And my cultivation did get better after using only store bought water.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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GenesisCorrupted
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Re: Ask Quick Questions, Get Quick Answers [Re: mushman509]
#28669827 - 02/21/24 02:05 PM (4 months, 1 day ago) |
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Just leave it alone. Somewhere dark. Off the ground.
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LadysKnight
Hello Ladies


Registered: 10/09/15
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] 2
#28669831 - 02/21/24 02:08 PM (4 months, 1 day ago) |
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Quote:
Smellyhobbit said: Let’s pretend this problem has also been ongoing for 3 years and all sensible changes have been made without results. Flowhood and PC are being used. Many coir brands have been tried. Agar and spawn appear clean.
Tubs die before or right after first flush.
Have you tested the flowhood with open agar plates lately? You could try a sab for a run. You could try sterilizing a batch of coir. Otherwise something sneaky on a plate?
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 2,001
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28669838 - 02/21/24 02:13 PM (4 months, 1 day ago) |
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Quote:
Smellyhobbit said: Theoretically, if someone had followed sterile procedure perfectly up to spawn, and indeed had anexic spawn, but saw contamination after spawn to bulk, what could be the culprit? Are there environmental factors powerful enough to ruin a tub that was made the correct way?
Theoretically, are you suggesting that shoebox grows are fine but the problem exists only for bulk tubs? That would eliminate some of the variables. What about pf tek, bottle or bag grows? If you know that the spawn and techniques are good because they work on other types of grows we have a good starting point.
-------------------- Stoned Gummys | BRF Pucks | Primo Prepour Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight]
#28669841 - 02/21/24 02:16 PM (4 months, 1 day ago) |
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The grow op in question has tried both SAB and flowhood. All tests indicate the flow good is operating as it should. Results are identical between SAB and flowhood use. Plates appear picture perfect.
Current trajectory is to do a grow cycle with everything made with bottled distilled water instead of tap, and we are finishing out current in-progress projects with coir going through the PC before use.
It’s not me. I’m just trying to help. Guy’s been at it for literally years and it’s not getting better.
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
#28669842 - 02/21/24 02:17 PM (4 months, 1 day ago) |
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Quote:
ReverendMyc said:
Quote:
Smellyhobbit said: Theoretically, if someone had followed sterile procedure perfectly up to spawn, and indeed had anexic spawn, but saw contamination after spawn to bulk, what could be the culprit? Are there environmental factors powerful enough to ruin a tub that was made the correct way?
Theoretically, are you suggesting that shoebox grows are fine but the problem exists only for bulk tubs? That would eliminate some of the variables. What about pf tek, bottle or bag grows? If you know that the spawn and techniques are good because they work on other types of grows we have a good starting point.
Unfortunately right now it’s everything from pint of spawn grows to monos. It’s all of it.
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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GenesisCorrupted
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] 1
#28669844 - 02/21/24 02:18 PM (4 months, 1 day ago) |
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Ask if he is using tapwater or well water.
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Smellyhobbit
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It’s tap. I’m looking into the quality of tap in his area. It’s hard. Like really hard tap water, but that shouldn’t be a problem right?
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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Yahra
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28669850 - 02/21/24 02:23 PM (4 months, 1 day ago) |
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Quote:
Smellyhobbit said: It’s not me. I’m just trying to help.
1. That's very nice of you 2. Have you seen the "mold"? Maybe all is in order and that guy mistakes bruising for contamination?
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 2,001
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28669853 - 02/21/24 02:25 PM (4 months, 1 day ago) |
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Sabotage? 
No, I have watched a few people go through this on this forum and go half crazy trying to figure it out. I believe that true pasteurizing or substrate, even coir, has worked for some. Making sure that your substrate prep bucket or pot is only used for substrate or cleaned to the point of sterility after food or grain prep is another.
We have wicked hard tap water here and it doesn't cause me any grow problems. I use distilled for agar, li, lc and storage, but not sub or grain prep. But my mineral content could be different that this theoretical forest creature's.
-------------------- Stoned Gummys | BRF Pucks | Primo Prepour Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra]
#28669855 - 02/21/24 02:26 PM (4 months, 1 day ago) |
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Quote:
Yahra said:
Quote:
Smellyhobbit said: It’s not me. I’m just trying to help.
1. That's very nice of you 2. Have you seen the "mold"? Maybe all is in order and that guy mistakes bruising for contamination?
It’s mostly trich, which we all have in our houses all the time anyway. If it is like lemon is saying, it could be myc not recovering well due to the water quality in the coir and grain and then trich just coming in for a quick meal
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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Smellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
#28669856 - 02/21/24 02:27 PM (4 months, 1 day ago) |
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Quote:
ReverendMyc said: Sabotage? 
No, I have watched a few people go through this on this forum and go half crazy trying to figure it out. I believe that true pasteurizing or substrate, even coir, has worked for some. Making sure that your substrate prep bucket or pot is only used for substrate or cleaned to the point of sterility after food or grain prep is another.
We have wicked hard tap water here and it doesn't cause me any grow problems. I use distilled for agar, li, lc and storage, but not sub or grain prep. But my mineral content could be different that this theoretical forest creature's.
This is what bugs me. You can find tons and tons of people both blaming and ok’ing hard tap water here. But water will necessarily be different region to region so who knows.
PCing coir and using bottle water may work if it’s either of those problems. Good starting point.
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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GenesisCorrupted
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28669859 - 02/21/24 02:29 PM (4 months, 1 day ago) |
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So you’re saying it makes it to first flush usually?
Because I’m thinking, maybe when he takes the lid off, he doesn’t sanitize it completely when he puts it back on for that second flush.
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Yahra
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28669860 - 02/21/24 02:29 PM (4 months, 1 day ago) |
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Or try a different harvest technique. Cutting the stems vs pulling.
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GenesisCorrupted
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra]
#28669863 - 02/21/24 02:30 PM (4 months, 1 day ago) |
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That wouldn’t introduce trich though. Unless he hadn’t sanitized the blade.
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Smellyhobbit
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Quote:
GenesisCorrupted said: So you’re saying it makes it to first flush usually?
Because I’m thinking, maybe when he takes the lid off, he doesn’t sanitize it completely when he puts it back on for that second flush.
Dude I don’t even wash my tubs with soap. There are lids that 100% have been on triched out tubs that I let dry and then put right back into use.
There’s no usually here unfortunately. Sometimes it makes it to flush one, but it dies afterwards. A lot of the time it doesn’t make it that far.
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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syri
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra]
#28669867 - 02/21/24 02:32 PM (4 months, 1 day ago) |
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I twist them off at the base with my index and thumb.
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GenesisCorrupted
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28669868 - 02/21/24 02:34 PM (4 months, 1 day ago) |
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Quote:
Smellyhobbit said: Dude I don’t even wash my tubs with soap. There are lids that 100% have been on triched out tubs that I let dry and then put right back into use.
 Well, that’s news to me. Thank you.
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