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Re: Official Misc EXOTIC Thread [Re: scapo] * 4
    #28650562 -

Tripped over this earlier today. Added to journal and cross posted from the Pan thread where it seems to be a topic as well. 

Not sure about Pans, but with section Zapotecorum some pigmentation is not uncommon. It becomes more common with section Cordisporae and very prevalent across the entire Fagicola complex. The culprit is typically nutrients, but several other factors come into play. Different species, but much of this applies to our little friends. It's actually a pretty cool read too.
Observations on Some of the Mycelial Growth and Pigmentation Characteristics of Cordyceps militaris Isolates

From what I've been able to gather beyond this article, it may not be a bad thing to have at least a little pigmentation and we may want to avoid tomentose pure white mycelium as it may lead to non-fruiting cultures with some species.
:thatsinteresting:

I've seen little to no reference of pH levels but it may be there between the lines. Again likely tied to nutrient uptake.

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Re: Official Misc EXOTIC Thread [Re: the_chosen_one] * 3
    #28650613 -

The white tomentose idea is interesting. When I think back to my best performing mexicana and tampanensis cultures, the ones to show some discoloration/banding typically performed better than whiter counterparts. I suspect others would have a differing point of view or experience but brings creedance to that idea in my experience.

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Re: Official Misc EXOTIC Thread [Re: the_chosen_one] * 1
    #28650636 -

If we wanted to raise the pH of the agar how would you go about it? A micro amount of calcium hydroxide in the dH2O while making agar?

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Re: Official Misc EXOTIC Thread [Re: kirkeng] * 2
    #28650658 -

That's what I was thinking. Luckily I didn't have to adjust the water agar, but it would be nice to know we can adjust any recipe with materials already on hand. I'll have to try it with my next batch of MEA. Otherwise, I notice they buffer with nutrients on most labels.

kirkeng said:
The white tomentose idea is interesting. When I think back to my best performing mexicana and tampanensis cultures, the ones to show some discoloration/banding typically performed better than whiter counterparts. I suspect others would have a differing point of view or experience but brings creedance to that idea in my experience.



Yeah I caught that in the Zapo paper Alan had worked on and I thought I saw some other comments while looking for Guzman's observation of pigmentation. The rabbit hole is fascinating and I haven't researched pigment since my albino days. :lol:

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Re: Official Misc EXOTIC Thread [Re: the_chosen_one]
    #28650821 -

Yea the cordisporae species are the heaviest pigmenters for me so far. I put the fagicola complex into the sect. Cordisporae

I have now observed it in ps mexicana, ps caerulescens, my cf. papuana, and my ps azurescens and ps subaeruginosa as well (in LC for these two, not on agar)

Thanks for sharing!


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Re: Official Misc EXOTIC Thread [Re: the_chosen_one] * 2
    #28650826 -

the_chosen_one said:
Tripped over this earlier today. Added to journal and cross posted from the Pan thread where it seems to be a topic as well. 

Not sure about Pans, but with section Zapotecorum some pigmentation is not uncommon. It becomes more common with section Cordisporae and very prevalent across the entire Fagicola complex. The culprit is typically nutrients, but several other factors come into play. Different species, but much of this applies to our little friends. It's actually a pretty cool read too.
Observations on Some of the Mycelial Growth and Pigmentation Characteristics of Cordyceps militaris Isolates

From what I've been able to gather beyond this article, it may not be a bad thing to have at least a little pigmentation and we may want to avoid tomentose pure white mycelium as it may lead to non-fruiting cultures with some species.
:thatsinteresting:

I've seen little to no reference of pH levels but it may be there between the lines. Again likely tied to nutrient uptake.



Oh wow! What really stands out to me is the role of light here

Notice how their colonies did not pigment when incubated in the dark vs under light

That’s very notable imo. They observed that light played an inhibitory role with the cordyceps they were using, and the plates in the dark had faster radial growth.


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Edited by scapo (02/07/24 06:53 PM)

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Re: Official Misc EXOTIC Thread [Re: scapo] * 2
    #28651028 -

scapo said:
Yea the cordisporae species are the heaviest pigmenters for me so far. I put the fagicola complex into the sect. Cordisporae

I have now observed it in ps mexicana, ps caerulescens, my cf. papuana, and my ps azurescens and ps subaeruginosa as well (in LC for these two, not on agar)

Thanks for sharing!




Tampanensis and ovoideocystidiata have grown pigment for me as well.


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Question everything, it's very responsive.

culture storage

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Re: Official Misc EXOTIC Thread [Re: Nichrome] * 6
    #28651065 -

I've seen that pigmenting on agar before as well, these are some older photos of when I was working with an ovoideocystidiata culture a couple years ago.


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Re: Official Misc EXOTIC Thread [Re: Mycoplex] * 3
    #28651324 -

I remember the first time I got a P. mexicana culture from Stonesun and it turned brown so quickly I thought it was contaminated. But was it ever productive. My zapo culture that fruited very well had some tan coloration but nothing like that one.


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I need tropical cultures, ABM, V.v....!!!!

Well things don't always look as they are and things can be misread and mistaken for what they realy are so don't read too much into what I say since I might be mistaken myself. And remember I rarely ever give a definate answer.

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Re: Official Misc EXOTIC Thread [Re: trout] * 3
    #28651405 -

Perhaps instead of trying to outrun it, there’s a balance of pigment that could be useful. When pigmentation is heavy I do observe a near complete halt to the myc growth. I wonder if there is benefit to mild pigmentation vs none, or if mild pigmentation it’s an indicator of a favorable and productive myc.


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Re: Official Misc EXOTIC Thread [Re: kirkeng] * 3
    #28651424 -

kirkeng said:
The white tomentose idea is interesting. When I think back to my best performing mexicana and tampanensis cultures, the ones to show some discoloration/banding typically performed better than whiter counterparts. I suspect others would have a differing point of view or experience but brings creedance to that idea in my experience.



Interesting, what exactly do you mean by better performance? If you mean yield, that would kinda explain why my Tamps almost always gave me relatively low yields.

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Re: Official Misc EXOTIC Thread [Re: Adas] * 4
    #28651471 -

@scapo, I think your on to something, I’ve started to notice in my neoxalapensis the much darker brown pigmented sections even becoming more leathery and the blade having a harder time cutting through it, while the less pigmented is slicing right through easy as can be. So there is a noticeable textural change for me. Further observations with neo seem to be showing the dark brown discoloration becoming its own “layer” of sorts with fine white threads above. Mine haven’t seem to have halted though at least on the current t2. As well transferring from darkly pigemented sections continues on normally after transfer. I should have taken pictures before I made transfers but most recent cut was sectoring into several distinct areas all showing differing levels of pigmentation, where as said before the darkest being harder to slice through.

@adas, yes yield has been noticeable better for me with pigmented cultures. The few times I’ve ran very white ones I’ve had a pretty substantial decrease. Granted I don’t want to fully attribute it all to culture coloration as other factors are always at play, but just been something I’ve kept duly noted for a while.

Edited by kirkeng (02/08/24 09:21 AM)

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Re: Official Misc EXOTIC Thread [Re: kirkeng] * 4
    #28651636 -

I've noticed the same texture thing. :lol: Thought it was a blunt blade but it was just as bad with a new one. Heimii and Yungensis are like leather.

I'm thinking the balance is what we are looking for too. I noticed the most common pigment is melanin which would mostly protect it from light. Exposure might even force the colony into a stasis mode which we are seeing as a stall. I see less of it with my cultures that are grown in mostly dark conditions and even less from those in total darkness.
That's just scratching the surface though. Melanin ties into several aspects of fungi that would affect the overall health of the colony from defense to feeding. I'm thinking eliminating it entirely would not be a good thing. 
Synthesis and assembly of fungal melanin
Sounds like Melanin is a solid that lodges up in the cell walls so it might explain the toughness of some culture. :lol:

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Re: Official Misc EXOTIC Thread [Re: the_chosen_one] * 5
    #28651676 -

Glad I’m not the only one noticing some textural change…

This Banderillensis is about what I am going to be seeking in my neoxalapensis as well the others in cordisparae eventually. Obviously I don’t know that this is what is ideal but my gut is telling me it’s a nice medium between pigmentation and vigor/healthy growth. In a way kind of reminds me of the concentric pigments in Mexi/tamps. Expressing their flavor but remaining more typical.



These are still young so I’ll see if they change much over time, they’re just beautiful though I find myself checking them out every morning..

I’ll have to shove some plates in the drawer to try that light exposure or rather lack thereof.

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Re: Official Misc EXOTIC Thread [Re: kirkeng] * 2
    #28651708 -

kirkeng said:
@scapo, I think your on to something, I’ve started to notice in my neoxalapensis the much darker brown pigmented sections even becoming more leathery and the blade having a harder time cutting through it, while the less pigmented is slicing right through easy as can be. So there is a noticeable textural change for me. Further observations with neo seem to be showing the dark brown discoloration becoming its own “layer” of sorts with fine white threads above. Mine haven’t seem to have halted though at least on the current t2. As well transferring from darkly pigemented sections continues on normally after transfer. I should have taken pictures before I made transfers but most recent cut was sectoring into several distinct areas all showing differing levels of pigmentation, where as said before the darkest being harder to slice through.

@adas, yes yield has been noticeable better for me with pigmented cultures. The few times I’ve ran very white ones I’ve had a pretty substantial decrease. Granted I don’t want to fully attribute it all to culture coloration as other factors are always at play, but just been something I’ve kept duly noted for a while.



Cool, and what about potency? My white culture is very potent but I often hear people compare Tamp's potency to Cubes.

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Re: Official Misc EXOTIC Thread [Re: kirkeng] * 3
    #28651714 -

That looks great, keep up posted! I see all of my plates with a different perspective now

I have not noticed the texture thing yet! I’ll keep an eye out for it.

Pigmented cultures can do very well when spawned to lc I have noticed. Perhaps a pigmented culture that has confirmed clamps taken to lc is a good place to start


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Re: Official Misc EXOTIC Thread [Re: scapo] * 1
    #28651735 -

@adas, I didn’t think they were too bad, I had some tested a while back and they were around 9-11mg/g if I recall. Nothing crazy or anything but had a kick to em. I suppose it would depend on what cubes they’re being compared to yknow, some of the the more generic ones definitely weaker than the tamps, but the modest ones were comparable I’d say, albeit I enjoy tamps much more than cubes.

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Re: Official Misc EXOTIC Thread [Re: kirkeng] * 2
    #28651737 -

Trust your gut!!!
:crankeyclaus:

I gather the halos are mostly a result of Chitin in the substrate which at higher levels can become an inhibitor. Again, the stall with no apparent cause. Seeing a few things on SDA is making things start to make sense. I was noticing a very pronounced enzymatic leading edge and less pigmentation with Valdivia the other day. Almost like it's out running itself looking for more nutrients. The water agar should be really interesting. :hehehe:

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Re: Official Misc EXOTIC Thread [Re: the_chosen_one] * 7
    #28651884 -

I have this one crazy sect. zap plate that has made it through lc, pf, and is now in substrate that had several pigmented ring sections. The myc rips



Excited to see your trials and piece this all together


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Re: Official Misc EXOTIC Thread [Re: scapo]
    #28651898 -

Yeah, that's one of the most unique section zapotecorum I've worked with. I love it. It does shred everything. Almost too fast on agar. It's on par for PF jars at about 30 days. I have one attempting to top fruit now and two more about a week from birthing and/or spawning. More LC in the clean room waiting for a chance at more pulls too lol. I'm leaning more towards keralensis at this point with it, but inski noted seeing the rings in some section zapotecorum where he is so maybe more hope than anything. So many mysteries. Exciting times!

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