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Invisiblemilkboy
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Registered: 05/01/21
Posts: 2,295
Re: Official Misc EXOTIC Thread [Re: YoshiTrainer]
    #28613147 - 01/07/24 03:26 PM (20 days, 7 hours ago)

Quote:

YoshiTrainer said:
Absolutely incredible Ekabpils, congratulations!



:whathesaid:
Holy fuck those dont even look real!
So cool :mushroom2:


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Offlinescapo
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Re: Official Misc EXOTIC Thread [Re: milkboy] * 3
    #28613459 - 01/07/24 09:50 PM (20 days, 1 hour ago)

Not exactly what I expected from yungensis but here we are! About 1.5mo old LC LME/Peptone. Pulled syringes and inoculated some 4oz PF cakes



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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: Official Misc EXOTIC Thread [Re: scapo]
    #28613515 - 01/08/24 12:04 AM (19 days, 23 hours ago)

What are these looking like on agar for you?


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Invisibleelasticaltiger
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Re: Official Misc EXOTIC Thread [Re: ekabpils]
    #28613541 - 01/08/24 01:50 AM (19 days, 21 hours ago)

Quote:

ekabpils said:
New Subtropicalis (not Zap) genetics grows from wild spores. By taste (no cucumber alkalinity) and structure (fibrous stalk), it seems to be Subtropicalis. Joker from wild swabs collections )






That is phenomenal :rockon:


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: Official Misc EXOTIC Thread [Re: elasticaltiger] * 8
    #28614125 - 01/08/24 01:46 PM (19 days, 9 hours ago)

Quote:

wavyedge said:
Trying out sterilized subs in these takeout dishes:



- neoxalapensis
- baeocystis
- pan bisporus
- pan cyan
- semps

Across these substrates:

- Hardwood sawdust
- composted manure
- straw pellets + coir (2:1)
- worm castings
- starter mix (potting soil)
- mulch
- rye grass seed
- Apple wood chips

All brought to field capacity (wood was soaked for a few days, rye grass seed was cooked as grain) as best I could.

I sterilized the substrates in jars as I would do grain, and from there on treated them as sterile substrates, (sterilized tools, work in front of hood).

:elmo: maybe we find something neoxalapensis or baeocystis eats more readily. The semps and pans are for comparison. I also saved controls in case the 2hr PC wasn't enough.




The 'winners' were fully or mostly colonized in about 3 weeks.

Straw pellets, manure, worm castings seem to have done the best for neoxalapensis and baeocystis. I have neoxalapensis, baeocystis, as well as the comparison semps, pan bisporus, pan cyans as well that have mostly colonized the sterilized substrate.

So I decided to case with plain verm and move to fruiting...

Semps and pans:




Neoxalapensis:




Baeocystis (cold fruiting 13-15c):





It's hard to see the baeocystis mycelium on the substrate but there are light wispy strands throughout most of the sub. Neoxalapensis is also weaker than semps and pans are somewhere in the middle. In the interest of moving this forward I wanted to get more moisture in there with the help of some moistened verm.

Now I'm not really expecting anything to happen at this point and the experiment isn't really proving anything definitively. But I've got the fruiting room and it will be something to check on.


Edited by wavyedge (01/08/24 04:12 PM)


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Offlinescapo
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Re: Official Misc EXOTIC Thread [Re: wavyedge] * 6
    #28614533 - 01/08/24 08:51 PM (19 days, 2 hours ago)



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Offlinescapo
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Re: Official Misc EXOTIC Thread [Re: wavyedge]
    #28614535 - 01/08/24 08:53 PM (19 days, 2 hours ago)

Excellent work!!

How do you maintain 58F?


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: Official Misc EXOTIC Thread [Re: scapo] * 1
    #28614550 - 01/08/24 09:07 PM (19 days, 2 hours ago)

Nice, what's your agar recipe? I usually don't see those colors with neos but I saw before you do.

It's still a work in progress, but I've been hacking away on a fridge/freezer trying to get it into shape since I had the baeo find in Dec. Hoping to at least fruit the ovoids.

Documenting my fridge thing here:

https://www.shroomery.org/forums/showflat.php/Number/28599748


Edited by wavyedge (01/08/24 09:08 PM)


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Offlinescapo
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Re: Official Misc EXOTIC Thread [Re: wavyedge] * 1
    #28614662 - 01/08/24 10:51 PM (19 days, 26 minutes ago)

Per liter of water: 10g agar, 10g lme, 5g peptone, 50mg gentamycin

I noticed none of them come out pigmented in R2A agar (lower nute recipe) - I think it has something to do with the amount of nutrients in the recipe and possibly the temps they're stored at


Edited by scapo (01/08/24 10:53 PM)


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
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Re: Official Misc EXOTIC Thread [Re: scapo] * 2
    #28614681 - 01/08/24 11:21 PM (18 days, 23 hours ago)

Here's what most of mine look like:



So that's about half the lme and half the agar I'm using, and I'm not using peptone in this batch.

Temps are consistent 68-70f


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OnlineLogicaL ChaosM
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Re: Official Misc EXOTIC Thread [Re: scapo] * 2
    #28614761 - 01/09/24 03:32 AM (18 days, 19 hours ago)

Those blue-green stunztii's are incredible.

So jelly :awepreciation:

My avatar features some wild stuntzii's. Still one of my favorite finds


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PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms:shroomeryhead:| Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm :tombstone: || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏                                                         
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Offlinescapo
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Re: Official Misc EXOTIC Thread [Re: LogicaL Chaos] * 2
    #28615074 - 01/09/24 10:24 AM (18 days, 12 hours ago)

Thank you! They look a lot different fruited outdoors, this same tub is the one that flushed a near full canopy indoors and the fruits looked different (they’re in the journal). Iuch prefer the outdoor fruited ones

That agar looks good so far! I transferted some dark pigmented neox and fagicola back to R2A yesterday to see if it takes off white and stays white. Yours does look very nice so far, maybe it’s the peptone? Still have some work to do and info to gather to isolate why these Cordisporae are tinting on some recipes. The gentleman who got (probable) pins on agar recently used just rice water and sugar, and his myc looks white


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Offlinescapo
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Re: Official Misc EXOTIC Thread [Re: scapo] * 6
    #28615076 - 01/09/24 10:27 AM (18 days, 12 hours ago)

Ps hispanica (sequenced 100% fimetaria) pinning in a bag outside



Pf cake spawned to a bag on 10/31/23 with sterilized manure, coco, verm, worm castings, straw, and hemp. Cut the top off the bag and placed outside 12/8/23, one month later here we are! Temps 35-55 outside


Edited by scapo (01/09/24 11:06 AM)


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OfflineMartytheMushroom
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Re: Official Misc EXOTIC Thread [Re: scapo] * 6
    #28615295 - 01/09/24 01:46 PM (18 days, 9 hours ago)



5 lib cap trays colonized. Anyone have any suggestions on a live casing?


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Invisiblealaskappalachian
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Registered: 10/22/19
Posts: 1,674
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Re: Official Misc EXOTIC Thread [Re: scapo] * 3
    #28615315 - 01/09/24 02:07 PM (18 days, 9 hours ago)

Quote:

scapo said:
Ps hispanica (sequenced 100% fimetaria) pinning in a bag outside



Pf cake spawned to a bag on 10/31/23 with sterilized manure, coco, verm, worm castings, straw, and hemp. Cut the top off the bag and placed outside 12/8/23, one month later here we are! Temps 35-55 outside




Hells yeah.  I waited a couple months for my last shoebox to pin before it got a weird surface mold so excited to see a relative time frame! 
1st zap master finishing up.
gym lutefolius doing its usual trailing, dotty thing.

caerulescens 1/2pt ready for a shake and have a couple of these TTBV2 1/2pts ready for G2G.

Finally get some oats again finally today if they're stocked back up.  Fingers crossed 🤞
makarorae barf plates finally germed too.  Happy fella right here.


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InvisibleinskiM
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Re: Official Misc EXOTIC Thread [Re: alaskappalachian]
    #28615325 - 01/09/24 02:14 PM (18 days, 9 hours ago)

Quote:

alaskappalachian said:

makarorae barf plates finally germed too.  Happy fella right here.




Nice, what is the greenish pigment?
The first image in my signature is a wild collection of that species 👍


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Invisiblealaskappalachian
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Re: Official Misc EXOTIC Thread [Re: inski]
    #28615330 - 01/09/24 02:20 PM (18 days, 8 hours ago)

Cheap food coloring.  I love brf pucks but these old eyes don't do so well with uncolored pucks for germ purposes.  Love your sig pics btw.  This finally gives me an opportunity to ask you what the lower left one is.  Been driving me nuts.


--------------------


"First we build the tools, then they build us."

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Invisiblethe_chosen_one
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Re: Official Misc EXOTIC Thread [Re: alaskappalachian] * 1
    #28615405 - 01/09/24 03:19 PM (18 days, 7 hours ago)

I believe it's Panaeolopsis sp.

Hopefully I win a gill/cap sample if I'm right. :hehehe:


--------------------
"Luck favors the observant." - Workman



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Invisiblethe_chosen_one
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Re: Official Misc EXOTIC Thread [Re: the_chosen_one] * 4
    #28615426 - 01/09/24 03:49 PM (18 days, 7 hours ago)

So I don't know lol. This was just f'n around with a test sub, but now I'm at a cross roads. I threw a thin casing on it to help improve moisture content. The original goal was to put it into an outdoor pot with more sub. I have a few more jars almost colonized so now I'm thinking of trying a fruiting in the cold room I have this year. I moved all the wood lovers in there the other day to avoid the upcoming freeze but I can make room for this and some containers of colonizing chips. I didn't have any luck fruiting the last ones I had indoors, but I didn't have the cold room then either. :shrug: I suppose it's worth a try.

Psilocybe Aztecorum in test substrate day 14


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: Official Misc EXOTIC Thread [Re: the_chosen_one] * 1
    #28615441 - 01/09/24 04:04 PM (18 days, 7 hours ago)

It's about to get pretty cold here on the coast, so id say send it in your cold room.


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