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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium] * 1
    #28497442 -

Well what’s it’s not is PE mycelium, which is all that matters. Transfer away from it until you get a clean plate


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28497456 -

Hi guys! could you help me with these germ plates? From where should i plan to take my first transfers? Ignore the one PE and NAT plate which i injected shit ton of spores accidentally. Do any of these look promising, especially the AA+ plates which seem to have some kind of aura before myc or is it just mold? Anyways here are the plates, hope you can see something from the pictures, i don´t know how you guys get such clear pictures!



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Re: AGAR ENVY! (Anything and All things agar!) [Re: dr.snuggles]
    #28497463 -

The aa+ might have some bacteria riding along


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28497471 -

Would i be still able to clean it or is it even worth a try?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: dr.snuggles]
    #28497473 -

It's always worth the experience.

If it doesn't clear up in a few transfers I would try a warm pour or a sandwich tek


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28497491 -

You mean this sandwich tek? What is a warm pour?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: huey.bluey]
    #28497538 -

That's the one! A warm pour is similar, but you're pouring liquid agar over the culture instead


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 2
    #28497585 -



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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #28497605 -

Man, I'm seeing all these good looking plates, but I can't get shit to germinate. Was gonna go put in a few hours at work, but I guess I'll make some cakes


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 1
    #28497615 -

I used to germ on brf pucks then put that to water agar before transferring to regular agar. Guaranteed clean culture that way.


--------------------



Edited by AspectOfTheCreator (10/09/23 05:50 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Charlie Manseon]
    #28497641 -

Charlie Manseon said:
Thanks for the response, what do you think about the black dots on the PE?
I  did notice when I was testing these  cultures on agar that I didn’t let the needle fully cool after flame sterilization  so as I squeezed the side of my syringe  to release only a few drops it steamed  out and kinda quick cooled the needle and splattered  and on all 4 trays that happen with I ended up with some type of contamination I have not seen.
Thanks again


Am I to understand that you had 4 plates open all at once?  You should only be opening them one at a time, doing your business quickly, and closing them back up.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #28497642 -

Germing on BRF is a life hack. I’ve seen growth from old syringes in less than 24 hours. It’s a great way to clean dirty swabs, too.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28497648 -

I feel kinda bad never ever tried BRF puck out for germination :3 maybe you have to teach me those ways some times hobbit


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shrimps] * 1
    #28497659 -

The real utility is for syringes and yucky swabs. Good for cleaning baccy plates too. I don’t really use them anymore since I’m past most of that tbh


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) *DELETED* [Re: Smellyhobbit]
    #28497743 -

Post deleted by Risky Panacea

Reason for deletion: :smile:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Risky Panacea]
    #28497752 -

No, those need more transfers before they're ready for grain. Ideally you want to take your xfers before your plates are all grown out like that, but you'll be fine doing them now.


--------------------
CV+

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MojaveMyc]
    #28497774 -

I want gentamicin for agar but its script only

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Re: AGAR ENVY! (Anything and All things agar!) [Re: huey.bluey] * 4
    #28497777 -

huey.bluey said:
I want gentamicin for agar but its script only


If you need antibiotics for agar work you're doing something very wrong

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RockinRobot] * 2
    #28497779 -

I said want

I also read ampicillin works well for bacterial infections. also requires script.

Warm pour
RogerRabbit said:
Another trick to use that helps even without antibiotic agar is to simply pour hot agar right on top of a contaminated plate. The heat seems to slow down the molds long enough for the mushroom mycelium to recover and climb up through the layer to break out on top. This usually only takes a day or two. As soon as the myc starts to appear on the top layer, 'shave' a small amount off and transfer to a new dish. Don't take any of the agar, but only scrape a bit of the myc off the top. This seems to leave the contamination behind 90+ percent of the time. Repeat the process if necessary. I've cleaned up lots of edible cultures from the wild this way.



RogerRabbit said:
Antibiotics aren't for green mold which is an imperfect fungi, they're anti-bacterial. True, sterile technique is foremost, but some of us are using prints that were left under the cap too long and have bacterial rods all over them. When the mushies germinate, so does the bacteria. Antibiotic agar is a godsend, imo.


Edited by huey.bluey (10/09/23 05:34 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28497835 -

0mar said:
Well I’m back these plates are from 9-30 I’ve changed my recipe, I did a single Z, I tried to stay as sterile as possible and yet… nothing but mold. Idk what to do anymore.


well dammit, that sucks dude. I'm still not sure if that's mold or just weak cube myc though. Your sterile procedure is definitely good, haven't noticed any weird satellite colonies in any of your pics. But it seems extra weird to have the same issue crop up after trying so many different sources when otherwise it seems like you're getting no contams. Still got your older plates sitting around?

edit: for reference i thought this plate was mostly mold, transferred one of the ropes off of it to be 'safe', and now the whole thing and it's brother plate are knotting/pinning


--------------------
The most important step a man can take. It's not the first one, is it?
It's the next one. Always the next step, Dalinar.

Edited by NotFromGeorgia (10/09/23 06:24 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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