SirHuntington said:

Anyone have recommendations on where to get reusable plastic (PP5) petri's. I can't seem to find any in bulk and the small quantities are charged a premium for and I don't want to go with glass because I know I'll drop them.

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SirHuntington said:

Anyone have recommendations on where to get reusable plastic (PP5) petri's. I can't seem to find any in bulk and the small quantities are charged a premium for and I don't want to go with glass because I know I'll drop them.

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SirPsycho got your back. This is fro. His post. He said Amazon I believe.

drVetker said:

SirHuntington said:

Anyone have recommendations on where to get reusable plastic (PP5) petri's. I can't seem to find any in bulk and the small quantities are charged a premium for and I don't want to go with glass because I know I'll drop them.

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SirPsycho got your back. This is fro. His post. He said Amazon I believe.

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SirHuntington said:

Anyone have recommendations on where to get reusable plastic (PP5) petri's. I can't seem to find any in bulk and the small quantities are charged a premium for and I don't want to go with glass because I know I'll drop them.

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TheAtlantean said:

SirHuntington said:

Anyone have recommendations on where to get reusable plastic (PP5) petri's. I can't seem to find any in bulk and the small quantities are charged a premium for and I don't want to go with glass because I know I'll drop them.

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Here.

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SirPsycho said:

TheAtlantean said:

SirHuntington said:

Anyone have recommendations on where to get reusable plastic (PP5) petri's. I can't seem to find any in bulk and the small quantities are charged a premium for and I don't want to go with glass because I know I'll drop them.

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Here.

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Damn, that's a great price and they look a lot more clear than the pp5s I got

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TheAtlantean said:

SirHuntington said:

Anyone have recommendations on where to get reusable plastic (PP5) petri's. I can't seem to find any in bulk and the small quantities are charged a premium for and I don't want to go with glass because I know I'll drop them.

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Here.

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SirPsycho said:

... Btw the codes you blanked out all point to the manufacturer

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haha fuck do i know
Someone mentioned when you posted they could read your address or something from the codes so I redacted a bit, hahaha.

CocaineBuffet said:

TheAtlantean said:

SirHuntington said:

Anyone have recommendations on where to get reusable plastic (PP5) petri's. I can't seem to find any in bulk and the small quantities are charged a premium for and I don't want to go with glass because I know I'll drop them.

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Here.

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Just a heads up. Microppose is suspect as hell.

I realized a couple years ago that their "self healing injection ports" are just rubber feet for the bottom of chairs for hardwood floors.

https://www.amazon.com/Abeillo-Furniture-Sticky-Protectors-Laminate/dp/B0B5R847RS/ref=sr_1_46?crid=17IZEVKALSEBR&keywords=rubber+feet+for+furniture&qid=1689821022&sprefix=rubber+feet%2Caps%2C185&sr=8-46

Their "FEA Filter Disc" stickers are something repurposed as well but I forget what the original use was for.

Vote with your dollar.

Edit: I am not also saying their products DONT work. Just get creative and shop around

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c10h12n2o said:

Working with Spores:
since we fruit in open air, we can assume that spores are dirty. if someone sends you a spore print, you can assume it is going to have contams on it in addition to the spores you want to cultivate. this does not mean you got a bad print, it means you got a NORMAL print. the same thing applies to syringes. for this reason, when inoculating something with spores, it is a good idea to use as little as possible, since we only need a few spores and the more solution/spores we use, the more contams will be present as well. i recommend people never putting spores directly to grain, since it is so easy to culture the contams

a sterile jar of grain is basically the microbiological jackpot for anything that can digest it (fungi, bacteria, etc), so we need to take care to reduce the chances of culturing the wrong microorganisms. basically, ALWAYS germinate your spores first on agar before applying them to grain/lc/etc.

by using streaking techniques, you can easily clean up even the dirtiest of prints or spore syringes. you basically are using a loop to spread spores VERY thin across the surface of agar, creating various zones with different concentrations of spores. this is extremely useful for cleaning up a print, since you spread the contams thin while you spread the spores. this is also a great way to obtain isolates about 100 transfers sooner than you would get from a blob of spores, since the later zones have very few strains present in each colony. by doing one of these serial dilution style streaks, you end up with hundreds of colonies to choose from, of various genetic diversity, which gives you lots of chances to select a vigorous, organized culture

Quote:

In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria(fungi in our case). Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.

Streaking is rapid and ideally a simple process of isolation dilution. The technique is done by diluting a comparatively large concentration of bacteria to a smaller concentration. The decrease of bacteria should show that colonies are sufficiently spread apart to effect the separation of the different types of microbes. Streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop. Aseptic techniques are used to maintain microbiological cultures and to prevent contamination of the growth medium.There are many different types of methods used to streak a plate. Picking a technique is a matter of individual preference and can also depend on how large the number of microbes the sample contains.

The three-phase streaking pattern, known as the T-Streak, is recommended for beginners.The streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop. The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been covered. The loop then is re-sterilized and the plate is turned 90 degrees. Starting in the previously streaked section, the loop is dragged through it two to three times continuing the zigzag pattern. The procedure is then repeated once more being cautious to not touch the previously streaked sectors. Each time the loop gathers fewer and fewer bacteria until it gathers just single bacterial cells that can grow into a colony.The plate should show the heaviest growth in the first section. The second section will have less growth and a few isolated colonies, while the final section will have the least amount of growth and many isolated colonies.

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examples of streaked plates. different labs use different standards for streaking. colonies thin as the dish rotates counter clockwise

the way i like to do it is very similar to what is described above. basically, take your loop (bod's DIY loop tek) and scrape off a small amount of spores from a print (or drip a drop from a syringe directly onto the loop) and streak a side of the plate like below. then flame the loop again, DONT SCRAPE MORE SPORES, rotate the dish, and drag the loop from the area you streaked previously to streak zone 2, then flame and repeat

this is the streaking technique i like to use. it is tremendously handy for cleaning up spore prints, isolation projects, and even cleaning up old LCs

if you are using a spore syringe as your starting point (or a LC you are trying to refresh), you want to be super careful not to add any additional liquid to the plate, otherwise it will roll around and distort the streaking pattern. so make sure you dont drip over the plate, you shouldnt be putting any more liquid into the plate than what sticks to the loop

Conclusion:

so that is pretty much it!! a bit lengthy, but i think i covered most of what i wanted to cover for my post #1000. please let me know if yall have any questions or need clarification about anything

i also welcome any additional tips & tricks, feedback, criticism, discussion, debate, haters (), or critical thinking

thanks everyone for everything yall have done to make this place such an incredible resource. im so grateful to live in an age where there is an online community to collaborate with on our hobbies

warm regards,
c10

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bodhisatta said:

...
after the pressure drops to 0 you can remove the lid of your PC and tighten down the cap on the bottle and take the foil off.

At this point you can store the bottle in the fridge to use later or start to bring it down to pour temperature.

if you store the bottle in the fridge all you have to do to use it again is microwave it until it's liquid again. don't try to microwave it on high for 8 minutes. do a few minutes then shake, then a few more then shake.

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MicroMycelium said:

Has anyone ever prepared their agar ahead of time regularly?

I just reheated the leftover agar in my media bottle from my last batch, and it was super convenient to just warm it up and pour it out.
Debating on just preparing an extra medium bottle or two in my PC when I have extra space in it.
Obviously possible contact with contaminants is a possibility, but assuming it’s sterilized and stays sealed until pouring, is there any other downside?

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Camera93 said:

That's why we streak in a pattern

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