Mycodemus said:

I would like some input on these plates. Where should I transfer from? There’s one natalensis, which is the spore plate. 3 semperviva, transferred once.

Also I have 4 bags of purchased sterilized rye. I was thinking I’d do 2 semperviva and 2 natalensis. Can I do a Josex poke to nocc them up? Or do I need to make sslc first? They have injection ports, so to use an agar wedge, I’d have to cut them open so if possible I’d like to avoid that.

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Mycodemus said:

I would like some input on these plates. Where should I transfer from? There’s one natalensis, which is the spore plate. 3 semperviva, transferred once.

Also I have 4 bags of purchased sterilized rye. I was thinking I’d do 2 semperviva and 2 natalensis. Can I do a Josex poke to nocc them up? Or do I need to make sslc first? They have injection ports, so to use an agar wedge, I’d have to cut them open so if possible I’d like to avoid that.

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I would take from the fastest most aggressive colony.

Also, I streak my plates like a few others around here, or do the good ol’ scrape of a spore print with a sterile blade and good ol tappy tap above the plate and in front of my flow hood.

Generally I get spores only and takes one or two transfers to get solidly, clean and healthy mycelium like these.

The plate with a tiny dried up mushroom is t1 away from the MS plate. I let the MS plate die, probably shouldn’t have but… oh well.

The plate with clean healthy mycelium is t2

I tried to clone the in vitro mushroom but who knows. I got a pretty good selection it seems. Super aggressive and quick to colonize the plate. Excited to see it on grain here soon.

Mycodemus said:

Oh that’s interesting. Should I go for a bit that’s sort of half and half?

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Personally I would go around 12, 1 and 2 o'clock

**here is a marked up pic

Mycodemus said:

Thanks, to be clear which plate do you mean?

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Mycodemus said:

Ok, should I do a normal small triangle with my xacto then?

Also, for educational purposes, why?

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