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Invisiblenatedawgnow
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SEMI-SOLID LIQUID CULTURE * 2
    #26689470 - 05/23/20 02:54 AM (13 days, 18 hours ago)

:chief:SEMI-SOLID LIQUID CULTURE:chief:

Hello everybody and welcome to my thread!

So I've been working recently on a novel LC tek for funsies and for science!

I'm calling it semi-solid liquid culture (SSLC). So I noticed that LC tends to not like a rough shake,
and also tends to sink to the bottom of the jar/bag until colonized a bit for a shake, only at this point can it withstand
the damage from rough shaking. My solution to this problem? Enter SSLC :rockon:

The idea is to add a small amount of agar to your LC. I tested a bunch of ratios from .1-.5% and settled on .2% as the best ratio.
I used .5g agar for a 250mL liquid culture for this batch. This makes a very viscous soup. Anymore agar and it will solidify completely.
The amount of nutrients can be whatever you usually use for LCs. These were made with .2% nutes as well.

For this round I made 2 LCs, one traditional as a control, and one SSLC. So here are the 2 broths pre agar wedge so you can see how they look:

Regular LC


SSLC


Each LC was knocked up with a TOC clone.

Here are some pics a week or so into colonization. Unfortunately the SSLC is
kinda hard to see in the photos:

Regular LC


SSLC


Ok so I knocked up some jars with each LCs on the 14th. For each LC I made a a control jar where I just dumped it in
and let it sit, a shake jar that I shook vigorously, and a roll jar that I gently rolled back and forth to mix the
LC/grains. Each jar got around 10mL of LC, I free poured so impossible to be exact but that's what I was shooting for.

Here are the comparison shots between each LC on day 4. Roll and shake jars are from multiple angles. SSLC jars are on the left:

Control jars:


Roll jars:


Shake jars:


As you can see the SSLC jars are all almost fully colonized and the SSLC control jar shows a lot more growth than the
regular LC. My theory is that the myc has a stronger foothold because of the thicker medium and because of this it can
withstand more vigorous shaking and is less likely to sink to the bottom of the jars because it clings to grains.
My goal with it was to be able to shake it vigorously starting out to soak grains so that the jar colonizes in days instead of a week+.

As a side experiment, I dumped 50ml of SSLC into 1 jar to see how it will colonize. Here you can see how the SSLC
just sits on top of the grains until I shake it:


Here it is at day 4:


The jar is fully colonized, definitely a lot of moisture but no actual pools of water. This jar was shaken right after dumping in the 50ml.

Well that's about all he wrote! You may say "why bother when LC works just fine?"

My answer is because it's a hobby, it's fun, try some new shit every once in a while.

Let me know what you guys and gals think and if you have any comments please share!


--------------------
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And therefore never send to know for whom the bell tolls; it tolls for thee.




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Invisibleeatyualive
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26689743 - 05/23/20 08:16 AM (13 days, 13 hours ago)

:rockon:


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Re: SEMI-SOLID LIQUID CULTURE [Re: eatyualive]
    #26690050 - 05/23/20 11:47 AM (13 days, 10 hours ago)

Right on man I did exactly this with plates a while back during li experiments. 


It looks like this colonizes much faster than the plates did.

I’ll prob make one of these today myself.


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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26690148 - 05/23/20 01:06 PM (13 days, 8 hours ago)

Just used up the last of my LC so this is perfect timing! I'll give it a whirl


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Re: SEMI-SOLID LIQUID CULTURE [Re: pureshrooming]
    #26690293 - 05/23/20 02:24 PM (13 days, 7 hours ago)

Feel free to post pics and what not here when you do!


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And therefore never send to know for whom the bell tolls; it tolls for thee.




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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26690793 - 05/23/20 06:41 PM (13 days, 3 hours ago)

Just pulled 2 jars of LC with agar, 1 jar of standard LC, and 2 jars of water to make LI out of the PC. Gonna toss a wedge in all of them from the same clone plate and see who comes out on top! I'll update this when I have some results.


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Invisiblenatedawgnow
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Re: SEMI-SOLID LIQUID CULTURE [Re: pureshrooming]
    #26690823 - 05/23/20 07:00 PM (13 days, 2 hours ago)

Nice what ratios did you do for the sslc?

I found, and should have included im the tek, that with telephone agar it was
necessary to pre boil my broth to make sure all the agar dissolved before pcing.

Is yours nice and thick?


--------------------
Any man's death diminishes me, because I am involved in mankind.
And therefore never send to know for whom the bell tolls; it tolls for thee.




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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26691321 - 05/23/20 11:55 PM (12 days, 21 hours ago)

Yep nice and thicc! I do a pre-boil also so I got it nice and clear. It is definitely thicker than I expected but easily shaken.

I did 500g water, 1g agar, and 4g of lme then split that up between jars


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Re: SEMI-SOLID LIQUID CULTURE [Re: pureshrooming]
    #26691394 - 05/24/20 12:37 AM (12 days, 21 hours ago)

Make sure to give it a good swirl every once in a while to mix the colonized myc in.
Just don't get your filters wet with it, unless of course you're LC is filterless (way I do it)


--------------------
Any man's death diminishes me, because I am involved in mankind.
And therefore never send to know for whom the bell tolls; it tolls for thee.




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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26692028 - 05/24/20 10:01 AM (12 days, 11 hours ago)

I’m going to buy hay and hulls but when I come back I’m getting in on this.

I just can’t decide which type to do.

Probably albino cambo or APEU.


--------------------
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InvisiblebodhisattaM
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Re: SEMI-SOLID LIQUID CULTURE [Re: A.k.a]
    #26692144 - 05/24/20 10:53 AM (12 days, 10 hours ago)

Nice write-up.

Agar in LC isn't very novel tho

https://www.shroomery.org/forums/showflat.php/Number/13781184/fpart/1/vc/1

And someone else recently made an adding agar to LC tek also saying it helped the LC stick to the grain giving faster recovery.
Ill see if I can find the thread it was the same exact concept.


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Re: SEMI-SOLID LIQUID CULTURE [Re: bodhisatta] * 2
    #26692252 - 05/24/20 11:50 AM (12 days, 9 hours ago)

If the only reference you can find is from 10 years ago I say it's relatively novel :shrug:

Nothing is entirely original anymore, that's just where we are in the evolution of the human mind


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Any man's death diminishes me, because I am involved in mankind.
And therefore never send to know for whom the bell tolls; it tolls for thee.




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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26692277 - 05/24/20 12:00 PM (12 days, 9 hours ago)

If you can find that other thread I'd be interested in reading it. I did a quick cursory
search from the past 5 years before posting the tek and pulled up nothing similar other than caps' sagari which is what gave me the idea


--------------------
Any man's death diminishes me, because I am involved in mankind.
And therefore never send to know for whom the bell tolls; it tolls for thee.




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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26692291 - 05/24/20 12:07 PM (12 days, 9 hours ago)

:takingnotes:


--------------------
Tek's I use

LAGM2020


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Invisibleeatyualive
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Re: SEMI-SOLID LIQUID CULTURE [Re: staytrippy420]
    #26692316 - 05/24/20 12:16 PM (12 days, 9 hours ago)

How fast of recovery improvement are you seeing? I think this would be great for bag culture. I feel LI and LC seem to seep through the cracks in bags a little more than jars. I’ve recently been getting 4 day colonization with LI using these new pour lids I bought. And this isn’t a variety that is generally fast. I also only did my initial shake after inoculating(#rubbedoneout)

Im also of the mind that the chunkier and thicker liquid inoculant is, the better it sticks to the grain and the faster it colonizes.

A lot of it has to do with the way you shake and roll jars. I usually fill my quarts really high for LI or liquids. I actually did a lower volume of inoculation than I normally do and it colonized in 4 days. 13 out of 19 jars did this. And the ones I didn’t shake quite as well are still at 75-90%. Ive since spawned those 13 quarts. This time I did 1 plate to 19 quarts where I normally do 1 plate to 10.




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Edited by eatyualive (05/24/20 12:52 PM)


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Re: SEMI-SOLID LIQUID CULTURE [Re: bodhisatta] * 2
    #26692338 - 05/24/20 12:29 PM (12 days, 9 hours ago)

Quote:

bodhisatta said:
Nice write-up.

Agar in LC isn't very novel tho

https://www.shroomery.org/forums/showflat.php/Number/13781184/fpart/1/vc/1

And someone else recently made an adding agar to LC tek also saying it helped the LC stick to the grain giving faster recovery.
Ill see if I can find the thread it was the same exact concept.



Looks to me like someone is unfamilair with the saying... "those who live in glass houses; probably shouldn't throw stones."


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Re: SEMI-SOLID LIQUID CULTURE [Re: LotKid]
    #26692362 - 05/24/20 12:38 PM (12 days, 9 hours ago)

Nice, Nate!


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Re: SEMI-SOLID LIQUID CULTURE [Re: verum subsequentis]
    #26692476 - 05/24/20 01:34 PM (12 days, 8 hours ago)

@eat- basically seeing full colonization between 4 and 5 days. Haven't done a bag
yet but that is next on the list.

@verum and lot- thanks guys :rockon:

"All that is old shall become new through rebirth."


--------------------
Any man's death diminishes me, because I am involved in mankind.
And therefore never send to know for whom the bell tolls; it tolls for thee.




Edited by natedawgnow (05/24/20 04:05 PM)


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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26692491 - 05/24/20 01:48 PM (12 days, 7 hours ago)

Hah, I just inoculated a small jar of snot consistency agar like a few days ago.


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Edited by poisoned (05/24/20 01:49 PM)


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Invisibleeatyualive
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #26692532 - 05/24/20 02:07 PM (12 days, 7 hours ago)

Nice! I’d definitely be interested to see your results. I love liquids! :rockon:


Hmm snot agar you say....


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