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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 1
    #28366011 -

More mono's would just make more dika's then you'd have a variety of strains and not an isolated one.


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 1
    #28366013 -

Stipe-n Cap said:
Gatekeep or die :rockon:


I at least appreciate you taking the time to respond and give me things to think about, unlike these other dorks.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69] * 1
    #28366019 -

Dikaryons can branch laterally to fuse with compatible monokaryons, perhaps this is what he's referring to? It's called Bullers phenomenon.

We've run a train on this thread long enough, I'm sure other folks have pics and other thing's they'd like to talk aboot.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28366025 -

Stipe-n Cap said:
Dikaryons can branch laterally to fuse with compatible monokaryons, perhaps this is what he's referring to? It's called Bullers phenomenon.


Would their be other monokaryons for them to laterally branch to if it was started from only 2 spores done under microscopic conditions?


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 4
    #28366028 -

You want pics? I got pics




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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushhead] * 3
    #28366031 -

mushhead said:
SirPsycho said:
mushhead said:
I can't believe this.
:smbfacepalm:


What are you? New here?


Completely new, first post. I think it's pretty genetic that everything is made out of DNA.
:aweyeah:



Diarrhea is hereditary.


It runs in your jeans.


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Question everything, it's very responsive.
culture storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #28366038 -



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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69] * 1
    #28366039 -

rumfor69 said:
Stipe-n Cap said:
Dikaryons can branch laterally to fuse with compatible monokaryons, perhaps this is what he's referring to? It's called Bullers phenomenon.


Would their be other monokaryons for them to laterally branch to if it was started from only 2 spores done under microscopic conditions?


No, not likely. Serial dilution and cell spreading should create enough separation between spores to prevent mating.
Should there be more than a handful of spores, water agar is used to slow growth to prevent accidental mating.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 2
    #28366042 -

Stipe-n Cap said:
rumfor69 said:
Stipe-n Cap said:
Dikaryons can branch laterally to fuse with compatible monokaryons, perhaps this is what he's referring to? It's called Bullers phenomenon.


Would their be other monokaryons for them to laterally branch to if it was started from only 2 spores done under microscopic conditions?


No, not likely. Serial dilution should create enough separation between spores to prevent mating.
Should there be more than a handful of spores, water agar is used to slow growth to prevent accidental mating.


Awesome, thanks for the answer!


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #28366061 -

Nichrome said:
mushhead said:
SirPsycho said:
mushhead said:
I can't believe this.
:smbfacepalm:


What are you? New here?


Completely new, first post. I think it's pretty genetic that everything is made out of DNA.
:aweyeah:



Diarrhea is hereditary.


It runs in your jeans.


:canthelpbutlaugh:


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushhead] * 3
    #28366068 -

The things I say and do are just genetic expressions. That's why I have so much diarrhea of the mouth.


--------------------
Question everything, it's very responsive.
culture storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 2
    #28366074 -

SirPsycho said:
Is the satellite, contam or tamp myc. Place your bets. Answer revealed tomorrow.





No definitive results yet, still taking bets



Cultures don't seem to be merging but the satellite hasn't changed colours and growth speed seems slow for contam.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 1
    #28366084 -

:pipesmoke2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome] * 2
    #28366088 -

Nichrome said:
The things I say and do are just genetic expressions. That's why I have so much diarrhea of the mouth.



--------------------

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28366103 -

โ€œFive events can potentially generate fertile heterokaryons (Fig. (Fig.2):2): outcrossing between homokaryons (heterothallism), outcrossing between a homokaryon and a heterokaryon via the Buller phenomenon, selfing between homokaryons (heterothallism), selfing via the Buller phenomenon, and intramixis (pseudohomothallism). โ€œ

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449030/#:~:text=During%20the%20Buller%20phenomenon%20(1,the%20nucleus%20of%20the%20homokaryon).

Am I misinterpreting this? because it does not limit a strain to only being the product of two monokaryons

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Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW] * 3
    #28366105 -

HILLBILLY OUTLAW said:
Nichrome said:
The things I say and do are just genetic expressions. That's why I have so much diarrhea of the mouth.





--------------------
Question everything, it's very responsive.
culture storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Chamo]
    #28366127 -

Chamo said:
โ€œFive events can potentially generate fertile heterokaryons (Fig. (Fig.2):2): outcrossing between homokaryons (heterothallism), outcrossing between a homokaryon and a heterokaryon via the Buller phenomenon, selfing between homokaryons (heterothallism), selfing via the Buller phenomenon, and intramixis (pseudohomothallism). โ€œ

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449030/#:~:text=During%20the%20Buller%20phenomenon%20(1,the%20nucleus%20of%20the%20homokaryon).

Am I misinterpreting this? because it does not limit a strain to only being the product of two monokaryons


I figured this is what you meant, see my above reply:

Stipe-n Cap said:
Dikaryons can branch laterally to fuse with compatible monokaryons, perhaps this is what he's referring to? It's called Bullers phenomenon.



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28366139 -

โ€œAlthough some sporocarps might have resulted from outcrossing via classical heterothallism, it is certain that none of the sporocarps resulted from selfing or pseudohomothallism. All (or almost all) of the hybrid sporocarps represented unique individuals.โ€

Does this mean that a โ€œunique individualโ€ is the same as an โ€œisolated strainโ€

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Chamo]
    #28366143 -

No, a sporocarp is a mushroom. They're referring to hybrid fruit bodies.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 1
    #28366150 -

And?

The study was on the different ways those mushrooms were formed.

Unique individuals were not formed only through monokaryotic mating

Ahh okay I understand now. Thanks

Edited by Chamo (06/19/23 07:16 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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