Chamo said:

I would like to clarify because I think my writing conveys me the wrong way. I am not trying to be a troll or an asshole. It is that I understand what you are all saying and that the genetics are more complicated than simply looking for sectors.

But I do know when I see a trend. When I describe consistency in a grow I am not saying consistently good. 9/10 of the grows are failures. But they fail consistently.

What I am saying that in my experience (from multiple spore sources and over the course of many years) that there is a consistent trend in the way they grow.

The mycelium colonizes the substrate the same amount of time. The pinset looks the same. The morphology of the mushroom is the same. The the flushes recover in the same time. The product weight is the same. Of course not exactly the same, as there are always variable. But down to less than an ounce of difference.

And through all of my time growing, it is the same example that I gave no matter the spore source. If I inoculate a Petri dish MS, and take 10 transfers every single grow will produce something different in morphology, product weight, colonization times, etc.

That inconsistency doesn’t happen with T100. It might be inconsistently bad, but consistent nonetheless.

Why is that? (Again, genuine question)

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Chamo said:

I understand you. Maybe it is that my goal is to not work with spores. I don’t collect spore prints. I really like to be able to grab a culture out of the fridge and know exactly what I’m going to get. And it has worked for me…for now. I don’t get the same consistency on transfers from the same dish early on.

I’m also not trying to breed anything, which was my understanding for the need to collect a dikaryon produced from two monos for a “pure” strain.

I just want to be able to throw some myc on a plate and know how it is going to perform.

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Chamo said:

I don’t think you can declare it is not an isolate. But I’ll leave it be. Have a good one

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Chamo said:

That inconsistency doesn’t happen with T100

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T100? Weak...

Chamo said:

It is my understanding that a strain is defined by the sexual reproduction of any compatible hyphae.

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Yes. So, an isolate is an individual strain. When the mycelium branches and grows the likelihood of selecting an isolate with your scalpel, or even a needle biopsy, is impossible.



Individual strains are microscopic. Any attempt to transfer even an imperceptibly small amount of material will still contain many strains.

Stipe-n Cap said:

What I'm saying is that an isolate is a single strain. Isolated, singular, with two monokaryotic donors creating a single dikaryotic strain. This is an isolate. If you could isolate single straina on agar, labs wouldn't employ tedious techniques like serial dilution or dedikaryotization to produce individual strains.

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Chamo said:

Is that a factor of time? I spent a year transferring plates to get something like 8 worthy strains. If I had lab equipment I wouldn’t do it this way either.

Everything I have read other than on this website says a strain is not limited to two monokaryotic donors.

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Chamo said:

Everything I have read other than on this website says a strain is not limited to two monokaryotic donors.

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Chamo said:

I spent a year transferring plates to get something like 8 worthy strains

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