Screwup said:

Yeah plus you should be testing LC before sending it to grain anyways probably lol

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Prolific Koi said:

Took left over MAZ Dark star and RW prints and put them together in a single dish. What can I expect? MAZ and RW together or a new variation? Just crossed my mind today that I might not get the same variations since spores inoculated together? Noob question sorry haha. I’m getting more spores from later flushes as well with the bmr.

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tholos said:

After getting agar to grain under my belt, my next interest lies in speeding up my colonization times of grain jars.

My first inclinations are toward:

1) LI
2) LC
3) Master grain jars

1) for LI I would sterilize water in a mason jar w/ a stir bar, cut up a plate and drop it into the sterile water, stir it on a hot plate (with no heat) and then noc jars via pouring the water into them

2) for LC I Create a 0.2% LME solution, do a Josex poke or a little wedge from agar, let it propagate, test on plates before sending to grain

3) Pretty standard agar to grain, wait for the jar to colonize and then 1/4 - 1/5 of the grain per jar to noc a new myco quart

Can anyone give me pros/cons of each method or insights from their individuals experiences?

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Primary_yellow said:

Hi brains trust,

Where I am, the day time temps are up around 25ºC (77ºF) but night time temps get down to freezing at the moment, and the temp outside is the temp inside. My grain jars are somewhat insulated in their cupboard but they nevertheless feel cold to the touch in the morning and I'm worried they'll never colonise.

Does anyone have any advice or a link to a tek to make a small incubator? I've only got two quart jars going at the moment and will ultimately spawn out to two small shoe boxes which would probably need to go in the incubator too I'm guessing.

Any help would be appreciated.

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