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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28240447 -

AspectOfTheCreator said:


Bottom of a no pour plate. 1% MEA. T0. Multispore. Inoculated with a tiny piece of PF tek cake. 7 days old. Already taken transfers from 3-4 o'clock. Does it look like satellite mold at 12 o'clock? Guessing that's what's preventing growth in that direction. Otherwise does it appear healthy? Just want confirmation. Will post again when transfers grow out.


It doesn't to my eye. Just looks like some different mycelium growth. As you said the transfers are what matters so keep your eyes on them. In the meantime just let this plate grow because some times you can spot more promising growth as the plate fills out (or transfer a plate pin if it matures enough).

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Re: AGAR ENVY! (Anything and All things agar!) [Re: drVetker] * 1
    #28240462 -

drVetker said:
Hi everyone,

Any idea what's going on here? What is this myc trying to run from?
I keep thinking the agar surface was too wet and it's trying not to drown.

This t2 plate is 18 days old going nowhere. Seemed it had no contam, but when I opend it up to toss this it smelled a bit cheesy.




Those little white specs all over the surface kinda look like bacteria but I'm more interested in why the myc looks sunken. What's your agar recipe?


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 3
    #28240883 -

I don't usually add color but these came out pretty nice. I'm having good success moving to hot pour. I leave them in the SAB with big bags of desiccant to remove the condensation. One dirty bird from the last batch. All clean so far for this run.


PDA low nutrient soft set


Fabric bags of desiccant


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope] * 3
    #28241428 -


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Re: AGAR ENVY! (Anything and All things agar!) [Re: nogoodnamesleft]
    #28241634 -

I've been trying to clean up some swab plates using 0.2% LME agar and water agar.

The growth on water agar is very light. I was expecting to see some rhizo growth - I saw that very early on in one plate, but then it stopped and turned into wispy thin growth. The top left plate is 0.2% LME, the other three are water agar.


Closer look at a water agar plate: the growth shows better with the light behind the camera


Should I transfer from the leading edge, or is this a toss?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bismillah]
    #28241721 -

Bismillah said:
I've been trying to clean up some swab plates using 0.2% LME agar and water agar.

The growth on water agar is very light. I was expecting to see some rhizo growth - I saw that very early on in one plate, but then it stopped and turned into wispy thin growth. The top left plate is 0.2% LME, the other three are water agar.


Closer look at a water agar plate: the growth shows better with the light behind the camera


Should I transfer from the leading edge, or is this a toss?


I have some WA cups going at the moment too..
From what I understand that light wispy growth is exactly what you want. No nutrients = fast, light growth looking for food. Outrunning contams hopefully.

I would say that rhizo growth you saw initially was the myc feeding off the original agar wedge.

Personally I would transfer from the leading edge. I see no reason these should be trashed.
(I am a noob, however well read, maybe wait for another more experienced member to chime in)
Great pics by the way.


--------------------
For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
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Edited by Soloist (03/22/23 05:32 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28241800 -

SirPsycho said:
drVetker said:
Hi everyone,

Any idea what's going on here? What is this myc trying to run from?
I keep thinking the agar surface was too wet and it's trying not to drown.

This t2 plate is 18 days old going nowhere. Seemed it had no contam, but when I opend it up to toss this it smelled a bit cheesy.




Those little white specs all over the surface kinda look like bacteria but I'm more interested in why the myc looks sunken. What's your agar recipe?


Hey Psycho, this is on Kat's PDA with active charcoal added.
Charcoal 5g
Dried potato flakes 5g
Dextrose 7.5g
Agar 10g
Destilled water 473g

So far almost all the transfers I put on this, 7 or something, didn't go anywhere, maybe 2. The rest look either like this, sunken and stalled or just plain contaminated.

I did in parallel Seeker's rice flour agar and with that much better growth and less contam.

I PCed both agar versions together and transfered in parallel to both on same occasions. So, I doubt it's the PC or the SAB/lack of care to keep everything sterile as posible.

To Kat's credit I have to say I have no clue what i'm doing here and did both in parallel to learn and experiment and for her agar I used store bought dextrose that is not pure but has 10 vitamins added to it and the potato flakes I got in the grocery store, the most unadulterated I could find are 99% potato but have emulsifiers, stabiliser, aroma and natriummetabisulfied added to them.
So fuck do I know if all these shits in the ingredients promote bacterial growth, kill myc ect.

I'm trying to figure out here why it failed so badly to learn something useful.

Edited by drVetker (03/22/23 06:30 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: drVetker] * 2
    #28241960 -





All of the above dishes were agar to agar on 03.16.23. Tomorrow will be day 7, question, does this growth seem to be going at a decent pace?

I understand that genetics, type of media, environmental factors, etc. will all play a large part in this, just asking generally, to compare to everyone else's experience.


BONUS:

The following are pictures of a lc syringe plated to agar from a vendor on the approved list. I intended to do two plates, the first plate, I fucked up horribly. Over squirted and then attempted to evenly spread the puddle across the agar

The second is placing two drops into the middle






How are we looking?

Edited by tholos (03/22/23 08:22 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos]
    #28242025 -

APE plate looks problematic but give it more time to grow out.

Same with JF plates but I think there are some leading edges of the first JF plate pic where you can get a couple transfers from. Around 12 O clock.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #28242055 -

CocaineBuffet said:
APE plate looks problematic but give it more time to grow out.

Same with JF plates but I think there are some leading edges of the first JF plate pic where you can get a couple transfers from. Around 12 O clock.



Thank you for the feedback

Here is another APE plate that I believe also shows the same sort of weird/stunted growth




Is this around where you were referencing for the 12 o'clock transfer?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos] * 1
    #28242069 -

Thats the spot. I wouldn't personally use a plate for each if I can just do 2 transfers to the opposite sides of the plate (if done correctly. If you are working in a SAB I would do a plate for each).

That APE plate actually doesn't look that bad. I would let it go for another week and you will probably have a better range of healthy growth to transfer from.

I am not in the camp that believes the earliest transfer is the best so I can sometimes take a more promising transfer from a more grown out plate. That works for me but might not be for others.

Edit: For the JF plate: The reason I like that 12 spot is because you have bare agar at the edge and won't have to extract a specific part of growth when you got 1 or more areas of growth touching the edges.

Edited by CocaineBuffet (03/22/23 09:24 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #28242101 -

CocaineBuffet said:
Thats the spot. I wouldn't personally use a plate for each if I can just do 2 transfers to the opposite sides of the plate (if done correctly. If you are working in a SAB I would do a plate for each).



Noted on the using more than 1 transfer per plate, I will try this next time I perform one

CocaineBuffet said:
That APE plate actually doesn't look that bad. I would let it go for another week and you will probably have a better range of healthy growth to transfer from.



This plate is actually 6 days older than the plate in the first picture

CocaineBuffet said:
I am not in the camp that believes the earliest transfer is the best so I can sometimes take a more promising transfer from a more grown out plate. That works for me but might not be for others.



The first plate was exactly this scenario: I had already taken a t1 6 days prior, but saw a very nice looking rhizo growth pattern toward the very edge of that original plate 6 days later, and decided to take a chunk of it and throw it on a new plate

CocaineBuffet said:
Edit: For the JF plate: The reason I like that 12 spot is because you have bare agar at the edge and won't have to extract a specific part of growth when you got 1 or more areas of growth touching the edges.


Would sooner be better so that those two growths don't run into each other?

Thank you again for all of the feedback

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos]
    #28242117 -

Yeah. The sooner the better in that scenario so you won't be thinking in the back of your mind if something else got caught in the mix.

This is something that I only run into with spore to agar plates. Everything after that is pretty cut and dry. You will be able to see which transfers are living and which are thriving. :thumbup:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet] * 6
    #28242279 -

Do you like Pink Floyd?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: stubb] * 4
    #28242421 -

Decided it was time to test my p. tampanensis LC:



3 drops, 3 clean growths!

Sadly a lot of condensation from moving them into a cold space for the pic and forgetting them :crazy:


--------------------
How I grow mushrooms πŸ„
How I do agar 🦠

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Re: AGAR ENVY! (Anything and All things agar!) [Re: stubb] * 4
    #28242425 -

stubb said:
Do you like Pink Floyd?




Dark Side of the Shroom

:starwars:


--------------------
Bod's Easy AF Search Engine TEK

Let's stay focused on what works consistently.

"Sitting in a bunker here behind my wall..."

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Re: AGAR ENVY! (Anything and All things agar!) [Re: myc_ousin_vinny] * 7
    #28242626 -

Does anyone say it like the bug alien in Men In black

β€œEgger”

Jumping in with some melmac revert/APE t1 lookin sexy,




Thai lipa yai tissue culture plate


Melmac clone i been runnin for years


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
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Re: AGAR ENVY! (Anything and All things agar!) [Re: myc_ousin_vinny] * 2
    #28242725 -



Yeti germ plate.  Pouring transfer plates today.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: KeyMaker]
    #28243248 -

Hello guys!

I am hoping you may be able to help me. I have been trying to progress from simple "home grow kits" (spore syringe into grain bag) to agar and home grain jars for best part of a year now, and have really been struggling with contam. I have slowly been upgrading my equipment, and now I think I am on the way.

I am having difficulty judging my agar plates - sometimes unsure what is contam and what is mycelium; and also having difficulty understanding how to judge healthy mycelium.

Would you guys be willing to talk me through the plates I have going at the moment? I have got rid of a dozen plates that were contaminated, leaving these ones:


I am most excited about this one. Should I do another transfer to agar? Or should I go to grain jars? Or possible to do both? If so, so you advise the leading edge of the thicker white mycelium?


This one ok?


I'm assuming from the vertical growth that this one is not so happy, is it salvagable, or should I transfer to another plate? Doesn't LOOK contaminated...?


This one I can't see any contam, but I do see condensation, which I know is a sign. It could be from it being no-pour I suppose? Am I missing something?


This one looks a bit messy. Transfer to another plate? Where is best to take from?

And THESE ones are from spores, so no transfers yet...


The growth at 10 o'clock - is that mycelium or contam? Should I transfer away yet?


This one appears to have some rhiz growth at the bottom, worth transferring? Is the thick fluffy stuff mycelium or contam?


Same as above: how to tell if this is contam or mycelium? And where to transfer from?


This looks good for transfers around 5/6 o'clock, right?


I think this is contam but just wanting to check...?


Thank you all. I am really wanting to learn more about how to identify mycelium.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: JSE] * 2
    #28243568 -

Pin grew right off the piece of agar I transferred. It wants to grow!



--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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