verum subsequentis said:

I suppose that means you don't have a hood. The idea seems sound, I just don't ever inject through anything.

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Drboomer said:

I mean if you are using the oven bag and pcing the ship in the bag it should be as close to sterile as it can get right? Or is it just that you doubt the sterility when going to pull the glc out since it's sat awhile?

I've been trying to come up with a way to inject into my setup for a needle biopsy without cracking the lid. I'm not really having contam problems doing it. But still would like to never open it. That's what the poly was for was to inject through but it constantly got wet. I initially tried to use a top cap to keep the poly sterile. Like this
But none of the epoxies held for very long the PC temps broke them down. Attempts to weld and braze we're not successful either.


I tried using a short thin needle on the syringe for the biopsy and a longer wider needle freshly sterilized and put into the luer lock bulkhead to keep the dirty from touching the clean but it didn't work.
Once I have a clean lc though these containers basically allow me to infinitely expand them keeping it sterile unless I fuck up. 

I like that I'm able to pull up big 60ml syringes, test them on agar and still keep them around capped and ready while I wait no potentially dirty ship.

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Yeah, it’s because the glc jars sit out for long periods of time.. the jars in the oven bag, I’m not worried about at all.. they are sterilized.

I like the idea of the capped lid.. I think josex uses/has used something similar.  The problem I have is that I already have a ton of culture in these lidded jars and it’d be nice if I didn’t have to make 48 new lids and the real effectiveness of the lids I use now is the amount of control I have with the GE.. I can tape up all the way or leave them I taped by 40% to dry the grains out after colonization. 

The left side is the exposed tyvek


And then in this pic you can see how I tape it up all but a sliver for GE during colonization


They’re also nice for printing because the print dries out really fast and I even put the jars in front of the fan to speed the process up.  I’m actually going to be doin g a thread about drying out bacterial cultures to clean them up.

But anyway.. I don’t want to derail the thread.  I appreciate you explaining that all to me

Faht

Lostkeys said:

For those of you who use activated charcoal in your agar mixes, what percentage of charcoal do you use? I tried making the black stuff once, but I'm fairly certain I used WAAAY too much charcoal and the growth was weak af.

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D3_Myc said:

Lostkeys said:

For those of you who use activated charcoal in your agar mixes, what percentage of charcoal do you use? I tried making the black stuff once, but I'm fairly certain I used WAAAY too much charcoal and the growth was weak af.

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I’d be lying if I said I measured it. I just grab a spoon out the drawer and a heaping half scoop. I think I estimated it to be 1g per 100ml. I’ve been hearing some complaints of activated charcoal possibly causing issues. I haven’t tried activated myself to verify.

The amazon listing says activated but the manufacturer has confirmed that this charcoal is not activated charcoal and it’s a very fine powder.

Smart Solutions Organic Activated... https://www.amazon.com/dp/B07JGCCTJ5?ref=ppx_pop_mob_ap_share

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fahtster said:

I actually get to post in the agar thread! Lol. I was gifted a couple mak plates (bk and tp).  I used the ole oven bag to do some transfers to glc grain jars and they’re taking off already.. probably knock up some qts in a week or so.


Faht

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Agar virginity? Haha still used the bag and sterile water. Nice job! You could pour in bags/transfer in bags LOL

semp looking stringy. Need dissolvable yeast and these plastic plates are trashed.

rockyfungus said:

fahtster said:

I actually get to post in the agar thread! Lol. I was gifted a couple mak plates (bk and tp).  I used the ole oven bag to do some transfers to glc grain jars and they’re taking off already.. probably knock up some qts in a week or so.


Faht

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Agar virginity? Haha still used the bag and sterile water. Nice job! You could pour in bags/transfer in bags LOL

semp looking stringy. Need dissolvable yeast and these plastic plates are trashed.

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