hogstomper said:

Got a few questions I'd like to drop here:

1) I want to test my LC (media bottle, regular lid) by sticking an innoc loop into the broth, then streaking on an agar dish.  Cool or FUCKED UP?

I would either poor a little or use a syringe to squirt a little onto the flame sterilized loop.

2) Is there any danger in handling your petri dishes too much?  Like do I need to wash my hands or be careful about/when handling them by checking on them 69 times a day?

If I pick up my plate, I always sanitize my hands with iso first.

3) If  plate pins, does that mean it's definitley clean?  Can I be sure that it's cool to "go to grain/LC" with a plate pin?

I have never gone straight to grain with a plate pin but I have put a few to LC. It works just fine.

4) Do I need to wrap plates inside the SAB?  I ask because I can never cut my saran cleanly and it causes me a huge headache.  Gonna get a sharp knife ASAP.

I don't use a sab so I cannot comment on this.

5) I get a lot of edge contams - can this be b/c my hands are wet with iso when handling them?  Also, it's so hard to move them without pawing the edges, lifting the lid briefly, etc.  Is there a good way to do this?  LIke they have anti-slide bottoms so it's almost impossible to just slide them and lift from the bottom.

Practice makes perfect. Just be conscious of your movements and make sure that nothing that is unsterile hovers over your plates.

6)Do you just roll up the empty sleeve inside the SAB to use later? It seems like germs would hop in it once opened?

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Atomsplit said:

Thanks AKA! Always appreciate it!

Here's another plate. Looks like there may be some chance of myc on the right transfer.



I'm looking at 4 o'clock.
Any help would be appreciated. I suck and think everything is mold.

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