AtomHeart said:

nogoodnamesleft said:

AtomHeart said:

Clone of the most potent strain I've ever run across....

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Is that PF classic? Best trip I’ve ever had of my
Life and it’s never been topped to date !!

Can’t wait to revisit it

Gorgeous culture

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Close...it's a strain of PF Redspore I've isolated...drops a reddish purple print instead of the usual baby shit brown that Redspore normally drops.  If you let the spores fly the whole tub turns a burgundy wine color.  Hehe.  Here's a print next to a normal Mars variety print.

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D3monic said:

I love how lazy I’ve been able to be in front of the hood. When in a sab I was iso wiping it down top to bottom inside and out, everything that went in got wiped down, fresh clean towel underneath ect ect. Shower before doing my work, wipe my arms down, a lot of breath holding. Now I just come down to the lab, grab a paper towel, drop my scalpel and punch on it. Iso them, drop into my glass in front of hood so they can dry and use the iso soaked paper towel to wipe my work surface down and my worklight hanging over flow just for good measure. Whole process takes less than 2 mins and I’m off and running. Only time I wipe my plates down is if I’m transferring to grain as I am bringing the dish over my jar and flicking wedges out. Figured better safe than sorry.

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footpath said:

Yup. I use GTs kombucha bottles straight up.

Pan myc.

Cube myc.

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Nice LCs man!

nmd_myco said:

D3monic said:

I love how lazy I’ve been able to be in front of the hood. When in a sab I was iso wiping it down top to bottom inside and out, everything that went in got wiped down, fresh clean towel underneath ect ect. Shower before doing my work, wipe my arms down, a lot of breath holding. Now I just come down to the lab, grab a paper towel, drop my scalpel and punch on it. Iso them, drop into my glass in front of hood so they can dry and use the iso soaked paper towel to wipe my work surface down and my worklight hanging over flow just for good measure. Whole process takes less than 2 mins and I’m off and running. Only time I wipe my plates down is if I’m transferring to grain as I am bringing the dish over my jar and flicking wedges out. Figured better safe than sorry.

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next time somebody looks at me funny for buying a lab grade flow hood before I'd ever germinated a spore, I'll link this post.

Also, gives me hope that last week's agar/spore efforts will be successful.

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Doctor Mario said:

LoadedFish said:

I may wait, then. The only reason I asked is because I finally have a culture I’m confident in, but my other culture seems to have contammed which ruined my plan to do 2 plates into 7 jars. I was worried about the clean culture hitting the edge by the time I can get another plate ready so I guess I’ll have to transfer from that too.

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Don't get too caught up in it like I did. I spent weeks making transfers trying to get that perfect plate when I really could have dropped a wedge and been over the hump. For whatever reason, I was under the impression that I needed a full plate. Plates kept contaminating before full growth and just kept transferring. Then this happened.

Doctor Mario said:

I confess that that tonight I was stoked to advance from agar to grain. I had a nice puck that I went to put in a grain jar and it fell off the scalpel and landed face down on a wet towel. 🤦‍♂️ So disappointing... I put in the jar anyway but I guarantee there is no way that its not gonna contam. Oh well. I should have another nice puck ready in about a week and a half.

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LotKid said:

I use a single piece about the same size as if i were transferring to another plate.

The trick is not waiting too long to start shaking. To give you an idea... if the piece of agar is the size of a dime... when the growth looks like the size of a quarter i'm shaking if i havent done so already. My first shake is usually maybe 3 days after knocking up the jar.

Once myc grabs onto a nice lil cluster of grains around the agar... shake. Then like 3 days later... shake it again. Your jars should finish faster.

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Utilizing that advice, I dropped some wedges about nickel-quarter size and waited until they looked like this.



I gave 'em a good shake and rolled the jars around to get the colonized oats to distribute a little better and when the myc grabbed onto another cluster of oats, they got shook and rolled again.



Once the colonized oats looked pretty well distributed through out the jar,

I left them alone for a week and came back to this.



Some of the wedges that went into these jars came from plates that had mold and bacteria growing in them. Obviously you want to stay well away from that part of the plate as to not accidentally touch it with the blade and work real slow and careful or your jar will end up like this.


Don't forget, if you have 1 good jar, you potentially have many more of the same culture if you G2G.

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