Passioncreekmyco said:

Active charcoal agar Avery albino

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Skeletal Rider said:

Noobie here,

So on the 24th of August I inoculated some plates using a 1.5 LME agr solution. i made up 7 plates. Knowing I forgot to PC them i dumped four of the seven and re-poured agar and inoculated again. On the plates that were not Pc'd I have this almost wispy white growth. The ones that were PC'd have a bunch of contamination. By logic that doesn't make any sense to me. And the white growth in the un-sterilized jars is not showing rhizomorphic growth as far as i can see. I have two of those plates that clearly show the growth on the path of inoculation. So, I'm just looking for some advice on what to do.

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tryptkaloids said:

Here's what you do - take pictures.

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tryptkaloids said:

I mean the other plates. If they weren't sterilized then they are probably also contaminated.

Make sure you are venting your PC.

Seems like its technique

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tryptkaloids said:

Bring it to a boil without the weight on. When you hear it start to hiss leave it on high for atleast 10min. Then put the weight on, let it start to rock and turn it down to just above medium so it maintains pressure

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tryptkaloids said:

Bring it to a boil without the weight on. When you hear it start to hiss leave it on high for atleast 10min. Then put the weight on, let it start to rock and turn it down to just above medium so it maintains pressure

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Myc_Hunt said:

tryptkaloids said:

Bring it to a boil without the weight on. When you hear it start to hiss leave it on high for atleast 10min. Then put the weight on, let it start to rock and turn it down to just above medium so it maintains pressure

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Lol, I've never done this but will definitely start.

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Inthepit said:

Myc_Hunt said:

tryptkaloids said:

Bring it to a boil without the weight on. When you hear it start to hiss leave it on high for atleast 10min. Then put the weight on, let it start to rock and turn it down to just above medium so it maintains pressure

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Lol, I've never done this but will definitely start.

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Well there you go, it has to vent for 10 minutes.
Weird deal but the temperature is not high enough without doing that.
Something about steam vs air.
"If there is air still in the vessel then the temperature will actually be lower than the pressure would indicate. It has to do with a scientific principle called “partial pressure”.

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neef said:

How are these looking? Still learning the agar ropes

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Mighty damn good!

Tormato said:

neef said:

How are these looking? Still learning the agar ropes

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Mighty damn good!

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ChRnZN said:

A few grain sized pieces of the leading arm between 9 and 11 might do well. Malt extract is recommended for all transfers. Potato is often slower than malt.

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