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Re: AGAR ENVY! (Anything and All things agar!) [Re: w00tmycelium]
    #26837693 -

Definitely either light or temperature.

D3monic posted some rw plates the other day that are super reactive to it. You could count 16 rings on it or whatever which was how old it is.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26837749 -

D3monic said:
I see it mostly in tomentos growth. Can almost count the rings and see how many light cycles.







Thats awesome, actually if its because of light and temp thats makes total sense, its the same phenomenon that happens to trees rings, in winter grows slower so the color is darker.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0ptiquest]
    #26837764 -

I was thinkin tree rings too, but so what happens if temps and light is consistent... that would be a worthwhile experiment

Edited by SawCeeJack (07/22/20 11:12 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26838052 -

c10h12n2o said:
im so far beyond managable. I was going through 500 every 10 days or so for a while. Now im doing about 300 a month so its much nore managable but still excessive

But such is pheno hunting

Rn im workin w like 28 varieties of cubes and 9 species, up to 15 culture projects for each. Just silly and rediculous

Dont do what i do unless you love agar work lol...  i really enjoy pheno hunting more than cropping out



@c10 you should do a guide or log on pheno hunting, what you look for, what you discard, methodology, etc:pipesmoke:


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My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ManifoldPrime]
    #26838081 -

Anyone seen the "flameless" transfers jik fibs has been doing on fb?
He's just gently brushing a sterile swab over the myc and then onto a fresh Petri. This way he's only transferring a couple of cells from the culture as opposed to any actual growth.
Reckons he's got a 6/6 success rate so far, and allows for greater accuracy with isolating...
Pretty interesting stuff

***EDIT***
I dunno if this link will work but here ya go

https://www.facebook.com/groups/345451222706638/permalink/625866091331815/

Edited by The Dalcassian (07/22/20 01:50 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian]
    #26838238 -

Hey guys.
I'm just commenting for easy access to this sub.
Having problems with contam lately and I need to get better at this.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian]
    #26838567 -

The Dalcassian said:
Anyone seen the "flameless" transfers jik fibs has been doing on fb?
He's just gently brushing a sterile swab over the myc and then onto a fresh Petri. This way he's only transferring a couple of cells from the culture as opposed to any actual growth.
Reckons he's got a 6/6 success rate so far, and allows for greater accuracy with isolating...
Pretty interesting stuff

***EDIT***
I dunno if this link will work but here ya go

https://www.facebook.com/groups/345451222706638/permalink/625866091331815/



I do 'flameless'. . .

I bought 10 #7 scalpel handles of AliExpress. . . Attached blades, made foil wrappers, with reinforcement for the blade end. . .

I PC them with agar. . . Have tested them after 6 weeks also. . . No contains. . .

It's nice knowing that more than just the blade tip is sterile. . . I can 'tap' agar off without knocking contams into my work. . .


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The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

–––––    ╭∩╮(-_-)╭∩╮    –––––
The Official Kiwi DMT and MAOI Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #26838716 -

It would be nice having that many sterile scalpels. I usually soak my handle down in iso then when it’s dry torch the entire blade and the part of the scalpel that locks the blade on. After that I just reframe the top of the blade.

It’s crazy how the tiniest bump can knock loose a ton of bacteria and spores.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ManifoldPrime]
    #26838789 -

ManifoldPrime said:
c10h12n2o said:
im so far beyond managable. I was going through 500 every 10 days or so for a while. Now im doing about 300 a month so its much nore managable but still excessive

But such is pheno hunting

Rn im workin w like 28 varieties of cubes and 9 species, up to 15 culture projects for each. Just silly and rediculous

Dont do what i do unless you love agar work lol...  i really enjoy pheno hunting more than cropping out



@c10 you should do a guide or log on pheno hunting, what you look for, what you discard, methodology, etc:pipesmoke:



I would love to read this aswell:begger:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Puduwoke]
    #26838796 -

Puduwoke said:
ManifoldPrime said:
c10h12n2o said:
im so far beyond managable. I was going through 500 every 10 days or so for a while. Now im doing about 300 a month so its much nore managable but still excessive

But such is pheno hunting

Rn im workin w like 28 varieties of cubes and 9 species, up to 15 culture projects for each. Just silly and rediculous

Dont do what i do unless you love agar work lol...  i really enjoy pheno hunting more than cropping out



@c10 you should do a guide or log on pheno hunting, what you look for, what you discard, methodology, etc:pipesmoke:



I would love to read this aswell:begger:



Me three


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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Fresh Prints]
    #26838892 -

I’ve done a few flameless transfers, it’s ok. Don’t really have a love affair with swabs though. More a hate hate relationship. Though haven’t had issues with myc to agar it’s always spore xfer because spore swabs are inherently dirty from touching fruiting body.

On deck for tonight



These celltreat 10packs are a PITA can’t wait till nest scientific are back in stock.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26838919 -

Why are they a pain, I gotta buy more soon, I use celltreat now. I wouldn't mind changing it up.


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #26838956 -

Just used to 20 in a sleeve and pour stacks of 40. So now I gotta deal with 4 packages of plates instead of 2. Quality control a little lacking too. Outside my plate bags look like someone drug them through the lawn. Litteraly. Dirt and even a blade of grass I’ve seen so far.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26839029 -

Holy crap
:cringe:


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa] * 1
    #26839204 -

Taking some xfers... turn  volume down a little . Had to cut video short cause a beatles somg came on and facetube wouldn’t let me publish it



Edited by D3_Myc (07/22/20 10:33 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26839621 -



Yeah this one sample here definitely has to be affected by light cycles.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: w00tmycelium] * 1
    #26839691 -

I hate to be mean but....we should ban for FB links


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Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ichugwindex] * 1
    #26839697 -

ichugwindex said:
I hate to be mean but....we should ban for FB links



How come?
I know I'm the most recent culprit.


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Here Lies This Individual




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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian] * 1
    #26839724 -

Avoiding FB would be great in general.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: w00tmycelium] * 1
    #26839741 -

Is that just because of how much potential exposure one could get?


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Here Lies This Individual




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