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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,223
Loc: Gaming the system
Last seen: 10 hours, 46 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Huldrelokk]
#26823033 - 07/14/20 03:04 PM (6 years, 3 months ago)

I made waaaay too many plates when I started. Now I usually just put 2 or 3 t1 wedges on the same plate, take whatever looks best a few days later and focus on that.

It’s much easier to g2g than to make 15 plates imo.

I throw old plates in a box in case I mess up super bad and need to go back. But that hasn’t happened, mostly I end up digging through them looking for pins to clone.

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Roger Clemency
Smile

Registered: 03/23/20
Posts: 2,005

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#26823123 - 07/14/20 03:45 PM (6 years, 3 months ago)

:whathesaid:

I did the same which is why I mention it to newer growers I see who pour a bunch of plates. Better to save those extra plates for clones and whatnot and just focus on a few transfer plates at first. I usually transfer twice away from spores to drop a wedge or 3 time to go to LC or for an LI plate.

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Sour grapes, sweet revenge
Heaven starts right where hell ends

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oculodextro

Registered: 04/10/13
Posts: 1,205
Loc: Space is the Place
Last seen: 4 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Roger Clemency]
#26823190 - 07/14/20 04:16 PM (6 years, 3 months ago)

I put a few plates in the fridge, some in the ground, and some to grain. If it's a really vigorous or special transfer it goes to slant.

I don't mind having 5-10 transfers of each genetics.

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The Dalcassian
Dirty Druggie

Registered: 01/04/17
Posts: 952
Loc: Emerald lsles
Last seen: 3 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: oculodextro]
#26823217 - 07/14/20 04:37 PM (6 years, 3 months ago)

I go by 2's, 2 plates get inoculated, 2 transfers from each plate moving forward until it's clean. I only have 10 glass petris and 12 small ball jars ("no pours") I have to really take care to take clean transfers but so far (aside from one mckennai print) I've always managed to do it in 4 transfers from spore.

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Here Lies This Individual

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Ambrose

Registered: 01/16/20
Posts: 585

Re: AGAR ENVY! (Anything and All things agar!)

[Re: The Dalcassian]
#26823381 - 07/14/20 05:56 PM (6 years, 3 months ago)

Have some old plates I forgot about like the ones above, is it worthwhile to try and make any transfers or are the cultures likely too old by now?

Edited by Ambrose (07/14/20 05:58 PM)

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coAsTal
Friend

Registered: 04/04/06
Posts: 2,970
Loc: 5b

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#26823383 - 07/14/20 05:57 PM (6 years, 3 months ago)

A.k.a said:

I made waaaay too many plates when I started. Now I usually just put 2 or 3 t1 wedges on the same plate, take whatever looks best a few days later and focus on that.

It’s much easier to g2g than to make 15 plates imo.

I throw old plates in a box in case I mess up super bad and need to go back. But that hasn’t happened, mostly I end up digging through them looking for pins to clone.

I need to take this advice-- I'm in the habit of making like three separate transfer plates from every decent looking one I get to aim for clean ones, and I'm burning through plates too fast that way.

Like you I have more leftovers that I just don't use for anything. Since I'm getting a better at eliminating contams during plate making, I gotta start economizing.

When you're a very small-scale hobbyist like I am, it's unreasonable to buy a 500-case of petri's, but buying 20-packs every 6 weeks gets expensive too.

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w00tmycelium
Mycelium Enthusiast

Registered: 11/28/19
Posts: 159
Last seen: 5 months, 13 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: coAsTal]
#26823474 - 07/14/20 06:37 PM (6 years, 3 months ago)

Has anybody ever seen blue bruising on mycelium on agar before? Or more likely would that be contamination?

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Huldrelokk
Stranger

Registered: 09/30/10
Posts: 28
Last seen: 6 years, 17 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: w00tmycelium]
#26823486 - 07/14/20 06:44 PM (6 years, 3 months ago)

I see where I was going wrong with the many transfers, ill revise that method. I actually sprung for the 500 piece case, the price difference per plate was insane compared to the 20 per sleeve. Thanks all for the advice

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mushboy
modboy

Registered: 04/24/05
Posts: 40,820
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: w00tmycelium]
#26823499 - 07/14/20 06:53 PM (6 years, 3 months ago)

w00tmycelium said:

Has anybody ever seen blue bruising on mycelium on agar before? Or more likely would that be contamination?

I've only seen bruised cultures from reactions to contamination or extreme environmental fluctuations. ..I'm sure it could randomly happen under normal conditions.

Got pics?

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Edited by mushboy (07/14/20 06:57 PM)

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Roger Clemency
Smile

Registered: 03/23/20
Posts: 2,005

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#26823544 - 07/14/20 07:17 PM (6 years, 3 months ago)

Almost all my pe6 plates would bruise on the wedge when the plates would get about 3/4 full. That's the only time I remember seeing it but those were clean plates.

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Sour grapes, sweet revenge
Heaven starts right where hell ends

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LunatikPenguin
Stranger

Registered: 04/24/20
Posts: 96
Last seen: 5 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Roger Clemency]

1
#26825976 - 07/15/20 10:44 PM (6 years, 2 months ago)

Got some decent growth going on my first transfer from the single GT mss plate that germed. Going to do a few transfers from this as soon as I get some time to pour some more plates.

Third transfer of this B+ looking nice, but was really awed when I looked at the T2 plate 😁

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w00tmycelium
Mycelium Enthusiast

Registered: 11/28/19
Posts: 159
Last seen: 5 months, 13 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LunatikPenguin]
#26826132 - 07/16/20 01:26 AM (6 years, 2 months ago)

This is a T2 plate, does this look like the rhizomorphic pattern I'm looking for?

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Tweeq
Mad Gardener

Registered: 06/07/18
Posts: 2,507
Loc: Netherlands

Last seen: 13 hours, 42 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: w00tmycelium]
#26826188 - 07/16/20 03:20 AM (6 years, 2 months ago)

You wanna transfer from the piece on the right; left side of it looks good. Also from the top; 6:00-ish.

Do it asap bc otherwise it'll be hard to not scoop stuff from the other patches too.

Give each tissue sample its own dish at this stage.

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w00tmycelium
Mycelium Enthusiast

Registered: 11/28/19
Posts: 159
Last seen: 5 months, 13 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tweeq]
#26826211 - 07/16/20 03:41 AM (6 years, 2 months ago)

Tweeq said:

You wanna transfer from the piece on the right; left side of it looks good. Also from the top; 6:00-ish.

Do it asap bc otherwise it'll be hard to not scoop stuff from the other patches too.

Give each tissue sample its own dish at this stage.

I think you are totally right. Prepping to cut in an hour roughly for all the 10 plates. This is the strongest I've seen so it seems worth it.

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Regis
Haben wir noch pepps?

Registered: 06/07/20
Posts: 274
Loc: Germany
Last seen: 2 months, 2 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tweeq]
#26826214 - 07/16/20 03:48 AM (6 years, 2 months ago)

AA+ vs. contam, who will win?

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w00tmycelium
Mycelium Enthusiast

Registered: 11/28/19
Posts: 159
Last seen: 5 months, 13 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Regis]
#26826218 - 07/16/20 03:54 AM (6 years, 2 months ago)

Where's the contam?

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w00tmycelium
Mycelium Enthusiast

Registered: 11/28/19
Posts: 159
Last seen: 5 months, 13 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: w00tmycelium]
#26826238 - 07/16/20 04:10 AM (6 years, 2 months ago)

Legitimate new post and also what the hell do I do with all this good culture?

I only have so many plates and whatnot. Should I just spread everything everywhere? Probably.

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,223
Loc: Gaming the system
Last seen: 10 hours, 46 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: w00tmycelium]
#26826408 - 07/16/20 09:04 AM (6 years, 2 months ago)

You get used to thoughtlessly destroying tons of healthy myc.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#26826813 - 07/16/20 01:26 PM (6 years, 2 months ago)

So much destruction

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#26826880 - 07/16/20 02:10 PM (6 years, 2 months ago)

I took four fully overgrown plates, chopped'em up
and put them in the wife's flower pot. KO
By some stroke of luck, she was happy, and interested to see them come up!
:monkeyspank:

:monkeyspank:

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GETTING STARTED :sporedrop:

:sporedrop:

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