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 A.k.a
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 Re: AGAR ENVY! (Anything and All things agar!)  [Re: Culliganman360]
#26807932 - 07/06/20 09:05 PM (3 years, 9 months ago) |
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Looks good, did you clean those cups somehow or use them straight out of the package??
Yeah rice sized is perfect. I usually make a tiny triangle, as small as I can pick up. It’s probably easier to start a little bigger til you get used to handling the wedges. The smaller you go the less likely you are to pick up contamination and bring it with you to the new plate, plus it narrows down the culture more so it’ll be uniform growth much quicker ime.
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 Tormato  
The Goddess Kali Meh 😛
Registered: 07/01/17
Posts: 6,067
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
#26807959 - 07/06/20 09:21 PM (3 years, 9 months ago) |
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Quote:
Inthepit said:
Quote:
Tormato said:
....if it's not growing like a wild fire it's pretty safe to say it's the real deal.
So I'm up to T4 and average every 5 days to transfer.
That seems really fast, although I've heard Oyster grows fast.
Last attempt I think was just growing some kind of contam.
So does this qualify as "growing like wild fire"?
There're still not very clean looking. And too bad the shark fin wedge isn't visible in TD4.2. LOL
Shark fin wedge = a wedge that lands on edge and looks like a fin.
Those actually look good! Nice job! And shark fins happen....it'll be fine 
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Culliganman360
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
#26807999 - 07/06/20 09:50 PM (3 years, 9 months ago) |
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Straight out the pack bro . I figure it’s getting pc’d anyways why clean it
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Culliganman360
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Culliganman360]
#26808015 - 07/06/20 10:09 PM (3 years, 9 months ago) |
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Old plate pic kinda blurry but looks like healthy myc growing over the black mold . I remember that the mold was there and the myc grew to it
So would it be worth it to try and transfer sum of that . In hopes of getting a culture that fights off contams better like the natalensis strain...
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Tormato
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Culliganman360]
#26808043 - 07/06/20 10:30 PM (3 years, 9 months ago) |
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Quote:
Culliganman360 said:
Old plate pic kinda blurry but looks like healthy myc growing over the black mold . I remember that the mold was there and the myc grew to it
So would it be worth it to try and transfer sum of that . In hopes of getting a culture that fights off contams better like the natalensis strain...
I wouldn't....you'd just be transferring more mold.
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Bsdgaou
Registered: 02/20/19
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
#26808096 - 07/06/20 11:16 PM (3 years, 9 months ago) |
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10g agar, 10g DME, 450 ml water. Is this okay after 4 days? Seems a little thin. What are those white dots on mycelium?
Also, how do you deal with deposits, do you blend agar and DME? I just mix it with spoon.
Edited by Bsdgaou (07/06/20 11:18 PM)
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roarkell
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bsdgaou] 1
#26808287 - 07/07/20 05:10 AM (3 years, 9 months ago) |
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D3monic mentions in his agar tek to use an immersion blender... lucky enough my wife is a pastry chef... lol... my agar turns out much clearer with a lot less sediment than ever before.
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w00tmycelium
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bsdgaou]
#26809352 - 07/07/20 03:44 PM (3 years, 9 months ago) |
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Quote:
Bsdgaou said:
10g agar, 10g DME, 450 ml water. Is this okay after 4 days? Seems a little thin. What are those white dots on mycelium?
I've had various spores do that (white dots) on some of my plates but it does look kinda thin, but also seemingly normal for 4 days total in terms of growth rate. What temperature are you keeping them at?
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mushboy
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Re: AGAR ENVY! (Anything and All things agar!) [Re: w00tmycelium]
#26809416 - 07/07/20 04:26 PM (3 years, 9 months ago) |
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White dots sounds like bacteria colonies. Pix?
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D3_Myc
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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
#26809552 - 07/07/20 05:44 PM (3 years, 9 months ago) |
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mushboy
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] 1
#26809561 - 07/07/20 05:48 PM (3 years, 9 months ago) |
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That's clear as fuck bruh.
I always get similar results by using cold water
I'll try the immersion idea tho
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oculodextro
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] 1
#26809562 - 07/07/20 05:49 PM (3 years, 9 months ago) |
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Damn did I just transport back to the days of scene people!
Is this a catanmation.
T5 Lions Mane, plates clear enough I just use near boiling water from my water dispenser.
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D3_Myc
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
#26809566 - 07/07/20 05:50 PM (3 years, 9 months ago) |
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Depends a lot on your ingredients source too, obviously choppy fibrous agar agar going to give more fibrous results same with nutrients. I been stick blending a good minute or so
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Inthepit
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
#26809585 - 07/07/20 06:02 PM (3 years, 9 months ago) |
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Are your plates kept where there is no variation in temperature?
I'm thinking condensation is unavoidable with room temp swings.
Oh, and team telephone agar!
Edited by Inthepit (07/07/20 06:11 PM)
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DutchMyco
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
#26809593 - 07/07/20 06:07 PM (3 years, 9 months ago) |
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Does anyone use 'plant agar'? Agar sold for plant tissue culture, seems to be pure agar, but might have a purity difference to bacteriological agar. Would there be any issues for using this for fungi at all?
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oculodextro
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
#26809631 - 07/07/20 06:26 PM (3 years, 9 months ago) |
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Quote:
Inthepit said:
Are your plates kept where there is no variation in temperature?
I'm thinking condensation is unavoidable with room temp swings.
Oh, and team telephone agar!
Do you pour? Or No-pour?
I just learned to fight condensation in a dry climate, I can't imagine working with humidity in PR.
My secret is while my agar is cooling allow the plates to cool face down on a rack above the bottom of a SAB, or I guess those lucky with flowhoods you can have it blow over the plate.
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D3_Myc
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Re: AGAR ENVY! (Anything and All things agar!) [Re: oculodextro]
#26809656 - 07/07/20 06:36 PM (3 years, 9 months ago) |
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In the Sab i’d cover arm holes and let the plates sit for a few days unwrapped. With the flowhood a few hours in front the flow hood. Sometimes I’ll get a little fog but clears up quick. Lab RH about 66% since I share it with fruiting space.
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Inthepit
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Re: AGAR ENVY! (Anything and All things agar!) [Re: oculodextro]
#26809663 - 07/07/20 06:42 PM (3 years, 9 months ago) |
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Quote:
oculodextro said:
Quote:
Inthepit said:
Are your plates kept where there is no variation in temperature?
I'm thinking condensation is unavoidable with room temp swings.
Oh, and team telephone agar!
Do you pour? Or No-pour?
I just learned to fight condensation in a dry climate, I can't imagine working with humidity in PR.
My secret is while my agar is cooling allow the plates to cool face down on a rack above the bottom of a SAB, or I guess those lucky with flowhoods you can have it blow over the plate.
I just went thru experimenting diff ways for pre-pour, terrible.
So I'm back to pouring in a SAB, 113F, possibly stacked with a sleeve over and left for a day or so.
They usually come out decent except for the top ones.
But it seems with temp swings from 78-85F it creates condensation.
Lately I've been covering the plates with printer paper, ok...
Next I might try a large cooler to stabilize temp.
Oh, and a dresser drawer might work too. I've been keep plates there that aren't inoculated.
Course I have about a 10% edge contam rate there, but that's another story...
Edited by Inthepit (07/07/20 06:49 PM)
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oculodextro
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
#26809666 - 07/07/20 06:44 PM (3 years, 9 months ago) |
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Try a hotter mug or shot glass on top
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polaritymind
relaxed attention
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Re: AGAR ENVY! (Anything and All things agar!) [Re: oculodextro]
#26809713 - 07/07/20 07:07 PM (3 years, 9 months ago) |
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Quote:
I just went thru experimenting diff ways for pre-pour, terrible.
What were your problems, always works out fine for me?
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