Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale

Jump to first unread post Pages: < First | | 164 | 165 | 166 | 167 | 168 | 169 | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 | 181 | 182 | 183 | 184 | | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Re: AGAR ENVY! (Anything and All things agar!) [Re: dirtydangus]
    #26528962 -

dirtydangus said:
Some more pics of my agar for some advice:

Here is the first petri dish. I thought this was mold but it has sprouted some good mycellium i think:

Bottom:


My guess would be to do a transfer from maybe 6 o clock or 9 o clock from this one

Top:



Petri 2:

Bottom:


For this one, maybe transfer 2oclock or maybe 7/8 o clock?

Top:


What do you guys think? does this look like something reasonably healthy or is that all just mold?


Doesn't help to show the top without putting some light on top.


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #26529138 -

I have a few agar plates that I transferred once already and now they are on second plate. No contams yet as it has been several days, but still quite small.
When is the best time to do another another transfer? Is it risky if its still small? About the size of a US dime. Too soon to transfer or is it too risky?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: woofwoof]
    #26529377 -

Hey guys. This would be mold, correct? All my plates of this specific strain are looking almost cottony. I used the same technique for my other dishes, a pe, and everything’s going smooth. Not sure if the condensation is causing this to fluff up. Thoughts? Any help is greatly appreciated!





Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Surf707]
    #26529390 -

There’s definitely cube myc in there.

Looks like you could get some clean transfers off


--------------------
LAGM2020

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26529399 -

I would grab a piece of the leading edge from the first picture at the bottom sound 5:45 on a clock face. There is some good rhizomorphic myc there you can see the little ropey strands

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: derek420]
    #26530874 -

Just want to thank this thread. I just made 9 germ plates of cubes... streaking the spores... analyzed the hell out of them with jewelers lenses attached to reading glasses and saw how dirty spores are. Out of those 9 plates, I saw three places I could grab clean mycelia. Now I have a bunch of plates growing out cleaner than I have seen in a while.

Many many thanks! I was too uncritical before.

T


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Edited by tedoro (03/12/20 08:43 PM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro]
    #26531987 -

tedoro said:
Just want to thank this thread. I just made 9 germ plates of cubes... streaking the spores... analyzed the hell out of them with jewelers lenses attached to reading glasses and saw how dirty spores are. Out of those 9 plates, I saw three places I could grab clean mycelia. Now I have a bunch of plates growing out cleaner than I have seen in a while.

Many many thanks! I was too uncritical before.

T


dang bro , makes me skeptical of my plates ha  , how many xs on that lense ?



also , need you guys advice as of why the cultures that were looking very rhizo and organized, now look so different and not really good and strong? could they have contamed in the transfers ? the one's that look rhizo were petri dish clones , not the cultures that were looking good , it's like the mycelium didn't like the same weak in nutes formula , could that be it ?

one changed was i was using baby water , and switched to drinking water , could that affect it so much ?

could a standar LME formula stabilize the growth ?





thanks


--------------------
...and when you loose control ; you'll reap the harvest you have sown.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: dmppb]
    #26532294 -

Those plates look good to me, they’re just sectoring off nicely.


--------------------
LAGM2020

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26532403 -

A.k.a said:
Those plates look good to me, they’re just sectoring off nicely.



thanks man , but not so nice as the plate they came from , that ok ? i'm feeling like it's not liking it , or expected the same pattern of growth maybe ? anyway , i'll try a more common agar recipe to continue transferring and hopefully get nice and more stable growth


--------------------
...and when you loose control ; you'll reap the harvest you have sown.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: dmppb]
    #26532642 -

dmppb said:
tedoro said:
Just want to thank this thread. I just made 9 germ plates of cubes... streaking the spores... analyzed the hell out of them with jewelers lenses attached to reading glasses and saw how dirty spores are. Out of those 9 plates, I saw three places I could grab clean mycelia. Now I have a bunch of plates growing out cleaner than I have seen in a while.

Many many thanks! I was too uncritical before.

T


dang bro , makes me skeptical of my plates ha  , how many xs on that lense ?



also , need you guys advice as of why the cultures that were looking very rhizo and organized, now look so different and not really good and strong? could they have contamed in the transfers ? the one's that look rhizo were petri dish clones , not the cultures that were looking good , it's like the mycelium didn't like the same weak in nutes formula , could that be it ?

one changed was i was using baby water , and switched to drinking water , could that affect it so much ?

could a standar LME formula stabilize the growth ?




thanks


Those look pretty good to me, id take a transfer or 2 and then drop most of the rest into some spawn.

There's a couple of dots but that probably from premature inoculation ... lol.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: C12ShroomMan]
    #26532693 -

cool , thanks dudes,

i got some chosen plates and formula to work with , should be fun

:evil2:


--------------------
...and when you loose control ; you'll reap the harvest you have sown.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: dmppb]
    #26533318 -

Quote:
dang bro , makes me skeptical of my plates ha  , how many xs on that lense ?


They are some clip on jewelers flip down magnifiers. two of them. $5 at harbor freight. Really helpful.

https://www.harborfreight.com/165x-jewelers-clip-on-eye-loupe-94364.html


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Edited by tedoro (03/13/20 06:58 PM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro]
    #26533472 -

Quick question for the guys that do a lot of agar work... what do you do with the plates after you take your transfers? I've got dozens that look kinda goofy so I dont want to waste the grain, but I also feel responsible for the organism I started growing and dont want to just toss the plates... is there an in between?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #26533482 -

lol I saw somebody that cased plates and it worked recently.

I’ve been just throwing them into the box they came in. I recently dug through them and found tons of pins so those are good for cloning.


--------------------
LAGM2020

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26533564 -

Or you can always put them somewhere in nature to go back to the earth. I used to bury all my stem butts and substrate. Kinda stopped towards the end of my last mycology runs.


--------------------

TEKs I Like

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: MadHatter333]
    #26533648 -



From small amount of gt spores.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Zen999]
    #26533950 -

One on right looks great, left is mold, transfer soon.


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #26534014 -

Thanks that's good to know n yup has been transferred.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: MadHatter333]
    #26534489 -

MadHatter333 said:
Or you can always put them somewhere in nature to go back to the earth. I used to bury all my stem butts and substrate. Kinda stopped towards the end of my last mycology runs.


I like this idea... my mother in law does a lot of gardening and composting... shes an old school hippy too so I can probably just have her compost it all... Thanks!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #26534610 -

Are these good enough to go to grain? They are sort of splotchy lookin, which has me concerned.

1


2


--------------------
LAGM 2021
LAGM 2020
Trade List

Extras: Filter Print Post Top
Jump to top Pages: < First | | 164 | 165 | 166 | 167 | 168 | 169 | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 | 181 | 182 | 183 | 184 | | Last >

Shop: Mushroom-Hut Substrate Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Re: mold in agar plate Anonymous 14,243 3 12/04/99 12:35 AM
by Anonymous
* Agar Anonymous 1,589 8 04/15/04 10:15 AM
by Bi0TeK
* First AGAR atempt log...
( 1 2 all )		 MushroomJoe 3,275 24 10/30/07 07:59 AM
by FGL
* basic agar question redshadow 917 2 01/30/06 11:45 AM
by Sinthetic
* Potato Agar Culture Tubes! *NEW* r2_ 1,845 13 01/01/06 03:21 PM
by r2_
* Agar emergency - myc growth but container has bacteria n the sides *DELETED* vinzy_a 1,378 10 09/28/06 08:06 AM
by vinzy_a
* Bacterial contams - agar Owl 3,569 3 09/21/06 02:16 PM
by RogerRabbit
* Fruit on Agar?? Yarry 5,806 17 01/22/04 09:44 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
764,697 topic views. 17 members, 9,255 guests and 232 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.013 seconds on 16 queries.