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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: psycho_nauticus]
    #26507060 -

My first time doing agar and i do not think i am succeeding. I streaked a couple plates and did a transfer from what i thought was something healthy growing, but i am not sure i did well.

Here is a B+ i did but it looks like fluffy mold:





Here is a PE one that i think is doing better, but it could just be all bacteria. I dont really see any good mycelium so im not sure about it:





Heres another PE sample that looks pretty bad. There is one area circled in red that might be ok to transfer? or maybe that is bacteria too.:



It looks like there may be something i can salvage from one of the PE ones, but i am not sure.

Sorry for potato quality pics.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Shroomwomb]
    #26507082 -

Shroomwomb said:
How long is too long to wait before transferring? I have 3 plates I was trying to isolate but I forgot to do the transfers like a week or two ago now they've grown to the edges of the plate. Still just as thin and wispy, how do you people get such dense growth!


As long as there aren't any visible contams, it's fine! Perfect world scenario, transfer before it reaches the edges of the plate, solely because the edges could potentially harbor a contam that isn't apparent. About dense growth, one of two ways. Either get lucky from the print and get thick growth off the bat, or transfer a few times until it thickens up. I had a culture that after 7 wispy-ass, thin-ass, weak lookin plates, bam 8th one was rhizo as hell!

dirtydangus said:
It looks like there may be something i can salvage from one of the PE ones, but i am not sure.

Sorry for potato quality pics.


Yeah the potato pics make it difficult. The first two PE look pretty good, but clearer pics are needed.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: MLPismyOPSEC]
    #26507091 -

Difficult to tell you what's going on without pics of the surface.


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #26507187 -

Using a print is MS

MS= multispore (lots of strains)
Vs
Isolate= single strain

Did you mean syringe?


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26507199 -

Don't know what y'all are talking about, here is a ninja star!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26507219 -

c10h12n2o said:
Did you mean syringe?


Yes, any opinion on noob results with a syringe vs a print?


--------------------
Start Here and Read as Much as You Can <<< BOD’s / SHROOMERY Knowledge Index



Trade List

ISO Cuban and Roatan Honduras Prints

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: SYF8]
    #26507382 -

Prints have more spores condensed and easier to see on the plate when doing a swab. Syringe is significantly less spores suspended in who knows what if you didn't make it yourself. Plus as a noob you don't run into the issue of putting too much solution on the plate. There are other benefits to prints but I am biased towards it. Not many seasoned mycologists on this forum use syringes unless selling them or they are filled with liquid inoculate.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #26507421 -

:whathesaid:

Exactly.


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26507815 -

I tried filtering the potato bits:

  0730 10g potato, boiled for 10 minutes, then 8g agar, 6 drops red.
  0750 coffee filter failed, it collapsed over the edge
  O755 again
  0805 failed, just potato, but clogged filter,even using a pre filter
  O806 again
  0820 50% potato, just going with whatever!  :shrug:  8g agar, 6 drops red, to just boil.
  0830 into Presto 23 Qt, 10 min vent
  0848 25 min PC
  0915 PC timer up, turned heat off
  0945 30 minutes till valve dropped, out with media bottle and tightened cap.
  1145 Agar @115 F

:monkeyspank:
Momma just wants clear agar!

I can't wait to get some Lite Malt Extract DME.
Mebbe Briess, anyways everything is hard to get here.
But I may have found a brewing supplier here.


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit] * 1
    #26507823 -

So you tried just filtering the potato juice (before agar)? That works for me when I want to be anal, but I use potato flakes (instant potatoes) when I make PDA.


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #26507830 -

Ya second try, just potato, and I had a screen filter before (prefilter).
      Mebbe this is the wrong stuff? 

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit] * 1
    #26507849 -

Inthepit said:
I tried filtering the potato bits:

  0730 10g potato, boiled for 10 minutes, then 8g agar, 6 drops red.
  0750 coffee filter failed, it collapsed over the edge
  O755 again
  0805 failed, just potato, but clogged filter,even using a pre filter
  O806 again
  0820 50% potato, just going with whatever!  :shrug:  8g agar, 6 drops red, to just boil.
  0830 into Presto 23 Qt, 10 min vent
  0848 25 min PC
  0915 PC timer up, turned heat off
  0945 30 minutes till valve dropped, out with media bottle and tightened cap.
  1145 Agar @115 F

:monkeyspank:
Momma just wants clear agar!

I can't wait to get some Lite Malt Extract DME.
Mebbe Briess, anyways everything is hard to get here.
But I may have found a brewing supplier here.


Hit up Misty West on the fb group. She has her own line of agar mixes. Might ship to you, pretty sure she ships internationally


--------------------

D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit] * 1
    #26507958 -

Inthepit said:
Ya second try, just potato, and I had a screen filter before (prefilter).
      Mebbe this is the wrong stuff? 


That's the right stuff, the only thing I do differently is I run my flakes through a food processor first to chop them into dust.


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: SYF8]
    #26507976 -

SYF8 said:
I've done some MS to agar and it didn't go so well.  The plates had high condensation when inoculating, and in hind sight the whole attempt was a mess.  I have new "dry' plates ready to go.  With my novice skill set, any opinions on what would be less error-prone to start with, MS or print?


I'm a newb too and i find that using syringe is messy for me also. Use sterile swab to nocc up with syringe. With that said my germination rate with prints is at about 70-80% right now, much less bacteria than the syringes. Only thing you may need to worry about is mold. Transfer early and youll be fine. My advice if you make messy plates with the syringe, use a print. Worked wonders for me.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #26507979 -

Hey that's what my wife suggested!
Next time I'll try the coffee grinder
And for extra credit mebbe the coffee filter will work.
:hatsoff:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26508035 -

Good way to utilise a syringe onto agar is put a drop in a sterile shot glass then use a loop to streak the plate. Works well for me and avoids all that liquid on your plate :thumbup:


--------------------
Back once again with the ill behaviour



Links I like

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Smoothcat]
    #26508073 -

Can someone explain to me the connection with crazy looking plates from spore prints... and how using too many spores makes the mycelia super fluffy... and takes numerous transfers to even it out?  Whats going on with that?


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro] * 1
    #26508098 -



So, here we have a bunch of different strains fighting over the agar.
It's chaotic and there's no fluid movement.



And, here, when we've isolated a strain, we see that it can take a pretty even course forward with the agar.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: footpath]
    #26508108 -

dogfood agar. ha.

So people do the streaking to limit the dogs initially?


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro]
    #26508114 -

Yeah. And to keep them away from cats and hamsters.

Extras: Filter Print Post Top
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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