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c10h12n2o
serial dilutor

Registered: 01/21/15
Posts: 3,200
Loc: the abyss

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Smoothcat]
#26505384 - 02/26/20 08:58 PM (6 years, 7 months ago)

You'll never turn back from #7 handles :smile:

:smile:

those things rock

--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide

"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Culliganman360
Stranger

Registered: 06/06/19
Posts: 854

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#26505405 - 02/26/20 09:22 PM (6 years, 7 months ago)

How do these plates look?
Plate #1

Plate#2

Plate#3

Whatsup with the rings?

Edited by Culliganman360 (02/26/20 09:52 PM)

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LadysKnight
Hello Ladies

Registered: 10/09/15
Posts: 3,295
Loc: Between me and you

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Culliganman360]
#26505422 - 02/26/20 09:34 PM (6 years, 7 months ago)

3 has mold on the left. The others look fine backlit, but need to see the surface to be sure.

--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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MH5109
Stranger

Registered: 09/28/17
Posts: 1,385
Loc: America

Last seen: 5 years, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LadysKnight]
#26505458 - 02/26/20 10:04 PM (6 years, 7 months ago)

One looks funky too. 7-9 o'clock.

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JessicaRabbit88
Loveable Nerd

Registered: 09/16/19
Posts: 78
Loc: Far Far Away

Re: AGAR ENVY! (Anything and All things agar!)

[Re: c10h12n2o]
#26505513 - 02/26/20 10:43 PM (6 years, 7 months ago)

I think I'm failing with my agar and am getting rather obsessed.

Anything salvageable on these?

Petri A

Petri B

Petri D

Petri E

Petri F

Petri G

Petri H

Your guidance and feedback is very much appreciatead!

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,223
Loc: Gaming the system
Last seen: 2 hours, 18 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: JessicaRabbit88]
#26505536 - 02/26/20 10:57 PM (6 years, 7 months ago)

You have all kinds of good growth on those.

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huangdeez
Newbie

Registered: 02/10/20
Posts: 54
Loc: USA

Last seen: 6 years, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: JessicaRabbit88]
#26505597 - 02/26/20 11:58 PM (6 years, 7 months ago)

Just take small pieces that are as far away from the contamination as possible and pray. I would guess they could be saved, it may just need a lot of transfers to become clean.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#26505607 - 02/27/20 12:08 AM (6 years, 7 months ago)

Almost perfect, so close to symmetrical

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tedoro
ToadStool Tender

Registered: 02/06/15
Posts: 2,274
Last seen: 5 months, 23 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#26506426 - 02/27/20 02:29 PM (6 years, 7 months ago)

Update on my clear leading edge transfer. The two first photos are that transfer grown out a few days. last photo is the sample plate. Looks like its cube. Might not be clean tho.

Interesting.

--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Edited by tedoro (02/27/20 02:30 PM)

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Shroomwomb
Chad Thundercock

Registered: 04/22/18
Posts: 63
Loc: Seychelles
Last seen: 6 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tedoro]
#26506465 - 02/27/20 02:59 PM (6 years, 7 months ago)

How long is too long to wait before transferring? I have 3 plates I was trying to isolate but I forgot to do the transfers like a week or two ago now they've grown to the edges of the plate. Still just as thin and wispy, how do you people get such dense growth!

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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Shroomwomb]
#26506597 - 02/27/20 04:14 PM (6 years, 7 months ago)

And so it begins..

first inoculation in months!!

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LadysKnight
Hello Ladies

Registered: 10/09/15
Posts: 3,295
Loc: Between me and you

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tedoro]
#26506611 - 02/27/20 04:21 PM (6 years, 7 months ago)

tedoro said:

Update on my clear leading edge transfer. The two first photos are that transfer grown out a few days. last photo is the sample plate. Looks like its cube. Might not be clean tho.

Interesting.

Looks like mold to me.

--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras:

Bobbit
Even Stranger

Registered: 05/30/18
Posts: 1,252
Loc: The outsideround

Last seen: 11 months, 20 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Shroomwomb]
#26506794 - 02/27/20 06:13 PM (6 years, 7 months ago)

Shroomwomb said:

How long is too long to wait before transferring? I have 3 plates I was trying to isolate but I forgot to do the transfers like a week or two ago now they've grown to the edges of the plate. Still just as thin and wispy, how do you people get such dense growth!

I forgot a shiitake plate full of contam, for 8 months. . .
Transferred to a fresh plate. . .
Took it's time to start moving again yet started. . .

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tedoro
ToadStool Tender

Registered: 02/06/15
Posts: 2,274
Last seen: 5 months, 23 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LadysKnight]
#26506879 - 02/27/20 07:14 PM (6 years, 7 months ago)

LadysKnight said:

tedoro said:

Update on my clear leading edge transfer. The two first photos are that transfer grown out a few days. last photo is the sample plate. Looks like its cube. Might not be clean tho.

Interesting.

Looks like mold to me.

Looks pretty much like my other cube plates. I'm no expert tho. But I've been doing plates for 10 years.

--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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Crackatoa
Stranger in a strange land

Registered: 04/01/19
Posts: 5,406
Loc: Over by your Mama's house

Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tedoro]
#26506895 - 02/27/20 07:23 PM (6 years, 7 months ago)

tedoro said:

Update on my clear leading edge transfer. The two first photos are that transfer grown out a few days. last photo is the sample plate. Looks like its cube. Might not be clean tho.

Interesting.

The first 2 resemble myc but the plate you took the transfers from does not.

--------------------

Crack's Tek's and Shit

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tedoro
ToadStool Tender

Registered: 02/06/15
Posts: 2,274
Last seen: 5 months, 23 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Crackatoa]
#26506922 - 02/27/20 07:36 PM (6 years, 7 months ago)

Crackatoa said:

tedoro said:

Update on my clear leading edge transfer. The two first photos are that transfer grown out a few days. last photo is the sample plate. Looks like its cube. Might not be clean tho.

Interesting.

The first 2 resemble myc but the plate you took the transfers from does not.

Crackatoa said:

tedoro said:

Update on my clear leading edge transfer. The two first photos are that transfer grown out a few days. last photo is the sample plate. Looks like its cube. Might not be clean tho.

Interesting.

The first 2 resemble myc but the plate you took the transfers from does not.

back a few days we all chatted about these plates of mine.

https://www.shroomery.org/forums/showflat.php/Number/26496493#26496493

Some folks thought my plates were healthy cubes... and that soft agar can cause clear leading edges, some thought the clear leading edge was a contam. But most thought the original plates were cubes. I've been poorly fruiting these plates. I got no question the third plate is cube.

--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

Edited by tedoro (02/27/20 07:38 PM)

Extras:

LadysKnight
Hello Ladies

Registered: 10/09/15
Posts: 3,295
Loc: Between me and you

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tedoro]
#26506946 - 02/27/20 07:47 PM (6 years, 7 months ago)

There could be mush myc in there, but the mold is dominant.
I have a culture with the same issues. I have not been able to clean it up. Got a few going in bottles to try to get a couple fruits to clone, but keeping my hopes in check.

--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras:

psycho_nauticus
Stranger

Registered: 01/26/20
Posts: 779
Loc: Incognito on Cloud 9
Last seen: 3 years, 3 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LadysKnight]
#26507023 - 02/27/20 08:30 PM (6 years, 7 months ago)

Just for my curiosity and learning, what is the sign that indicates mold is present? What in the culture indicates and shows that? So I can learn for identification purposes.

Also, what is the best place and temperature to keep your transfers and plates? Mine were all clear of condensation yesterday, but today I have very light condensation on the inside of the glass of my no pours. I am keeping them in a box in my room. Is it getting too warm or hot or something?

--------------------

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LadysKnight
Hello Ladies

Registered: 10/09/15
Posts: 3,295
Loc: Between me and you

Re: AGAR ENVY! (Anything and All things agar!)

[Re: psycho_nauticus]
#26507045 - 02/27/20 08:41 PM (6 years, 7 months ago)

psycho_nauticus said:

Just for my curiosity and learning, what is the sign that indicates mold is present? What in the culture indicates and shows that? So I can learn for identification purposes.

Mush myc grows in a radial pattern, out from the center, even the fuzzy type. Mold looks more like polyfil, just growing randomly to fill the space.

--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras:

SYF8
Professional Hobbyist

Registered: 11/16/19
Posts: 189
Loc: The Mars Hotel
Last seen: 2 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: psycho_nauticus]
#26507057 - 02/27/20 08:46 PM (6 years, 7 months ago)

I've done some MS to agar and it didn't go so well. The plates had high condensation when inoculating, and in hind sight the whole attempt was a mess. I have new "dry' plates ready to go. With my novice skill set, any opinions on what would be less error-prone to start with, MS or print?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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