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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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agar plates...
    #3767907 -

it has been sorta cold for these, I am making a TnT, just waiting for the aquarium heater from ebay. So is this normal, these are malt agar plates.









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ABSTRACT ART (Mine) http://nathanbelomy.com

Edited by Fluxburn (02/11/05 06:27 PM)

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Re: agar plates... [Re: Fluxburn]
    #3768209 -

in the limited amount of agar work ive done, those look fine by me


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Grumpy Old Man.

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Re: agar plates... [Re: Fluxburn]
    #3768234 -

the I would have to say they may be contaminated, the spread is far to uneven
unless you inoculated it really odd.

were you using a flowhood or a glovebox


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California Has Legalized Child Prostitution

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Re: agar plates... [Re: Prisoner#1]
    #3768247 -

like i said ive done very little work with agar. priz, is it best to shoot agar with a syringe or just scrape the spores directly from the print onto it?


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Grumpy Old Man.

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Re: agar plates... [Re: Yarry]
    #3768279 -

it's better in my opinion to use a print, the excess water from the syringe increases the odds of contamination.... if your doing it from a syringe as an only option you can remove a lot of water by standing it on it's plunger for several hours and then evacuate most of the water by pushing down on the barrel


to inoculate the plate push a drop out and drag it across the surface, make sure you squirt a little first to get spores into the needle


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California Has Legalized Child Prostitution

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Re: agar plates... [Re: Prisoner#1]
    #3768295 -

and if im using prints? just scrape it on there?


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Grumpy Old Man.

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Re: agar plates... [Re: Yarry]
    #3768322 -

there are a couple of method for doing it... I heat my loop, streak the agar
to cool it and make the wire sticky, run it across the print and then wipe a few
spores in several areas on the plate... the fast clean healthy spots get moved to
other plates


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California Has Legalized Child Prostitution

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Re: agar plates... [Re: Prisoner#1]
    #3768335 -

cool! now i gotta get myself some plates! thanks priz


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Grumpy Old Man.

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Re: agar plates... [Re: Yarry]
    #3768356 -

Hum ok, I just made these in a rubbermaid container with the lid off not breathing and a crapload of alcohol and lysol was sprayed. I had gloves and fresh clothes on. I just made my glovebox today from the container. I squirted some sryinge spores on these and was hoping for the best. It has been almost a week, but it has been so cold so I don't know, maybe its slow growth? I thought myco was only white but I am reading there is white bacteria?

I forgot to add that I finished my syringe on this so there was a little extra fuild so it was spread all over the place. I looked at it closey and it looks like mycillum


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ABSTRACT ART (Mine) http://nathanbelomy.com

Edited by Fluxburn (02/11/05 08:00 PM)

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Re: agar plates... [Re: Fluxburn]
    #3768604 -

Fluxburn said:
I squirted some sryinge spores on these and was hoping for the best. I thought myco was only white but I am reading there is white bacteria?
I forgot to add that I finished my syringe on this so there was a little extra fuild so it was spread all over the place. I looked at it closey and it looks like mycillum



I think squirting was you're problem here, I guess you've read the thread and
have picked up a couple tricks... you can turn your rubbermaid into a glove box
for about $10, dryer vent and rubber gloves, the proper application of duct tape
and a piece of plexiglass, you'll be set then... arag is pretty fun to work with
once you have the environment for it


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California Has Legalized Child Prostitution

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Re: agar plates... [Re: Prisoner#1]
    #3768632 -

yah, mine cost more like $30 but I live in a high priced area.


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ABSTRACT ART (Mine) http://nathanbelomy.com

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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