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Fluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA
Last seen: 13 years, 2 months
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agar plates...
#3767907 - 02/11/05 06:15 PM (18 years, 11 months ago) |
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it has been sorta cold for these, I am making a TnT, just waiting for the aquarium heater from ebay. So is this normal, these are malt agar plates.



-------------------- ABSTRACT ART (Mine) http://nathanbelomy.com
Edited by Fluxburn (02/11/05 06:27 PM)
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Yarry
Old Timer


Registered: 01/04/04
Posts: 23,762
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Re: agar plates... [Re: Fluxburn]
#3768209 - 02/11/05 07:09 PM (18 years, 11 months ago) |
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in the limited amount of agar work ive done, those look fine by me
-------------------- Grumpy Old Man.
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: agar plates... [Re: Fluxburn]
#3768234 - 02/11/05 07:13 PM (18 years, 11 months ago) |
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the I would have to say they may be contaminated, the spread is far to uneven unless you inoculated it really odd.
were you using a flowhood or a glovebox
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Yarry
Old Timer


Registered: 01/04/04
Posts: 23,762
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Re: agar plates... [Re: Prisoner#1]
#3768247 - 02/11/05 07:15 PM (18 years, 11 months ago) |
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like i said ive done very little work with agar. priz, is it best to shoot agar with a syringe or just scrape the spores directly from the print onto it?
-------------------- Grumpy Old Man.
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: agar plates... [Re: Yarry]
#3768279 - 02/11/05 07:22 PM (18 years, 11 months ago) |
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it's better in my opinion to use a print, the excess water from the syringe increases the odds of contamination.... if your doing it from a syringe as an only option you can remove a lot of water by standing it on it's plunger for several hours and then evacuate most of the water by pushing down on the barrel
to inoculate the plate push a drop out and drag it across the surface, make sure you squirt a little first to get spores into the needle
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Yarry
Old Timer


Registered: 01/04/04
Posts: 23,762
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Re: agar plates... [Re: Prisoner#1]
#3768295 - 02/11/05 07:25 PM (18 years, 11 months ago) |
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and if im using prints? just scrape it on there?
-------------------- Grumpy Old Man.
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: agar plates... [Re: Yarry]
#3768322 - 02/11/05 07:30 PM (18 years, 11 months ago) |
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there are a couple of method for doing it... I heat my loop, streak the agar to cool it and make the wire sticky, run it across the print and then wipe a few spores in several areas on the plate... the fast clean healthy spots get moved to other plates
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Yarry
Old Timer


Registered: 01/04/04
Posts: 23,762
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Re: agar plates... [Re: Prisoner#1]
#3768335 - 02/11/05 07:32 PM (18 years, 11 months ago) |
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cool! now i gotta get myself some plates! thanks priz
-------------------- Grumpy Old Man.
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Fluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA
Last seen: 13 years, 2 months
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Re: agar plates... [Re: Yarry]
#3768356 - 02/11/05 07:36 PM (18 years, 11 months ago) |
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Hum ok, I just made these in a rubbermaid container with the lid off not breathing and a crapload of alcohol and lysol was sprayed. I had gloves and fresh clothes on. I just made my glovebox today from the container. I squirted some sryinge spores on these and was hoping for the best. It has been almost a week, but it has been so cold so I don't know, maybe its slow growth? I thought myco was only white but I am reading there is white bacteria?
I forgot to add that I finished my syringe on this so there was a little extra fuild so it was spread all over the place. I looked at it closey and it looks like mycillum
-------------------- ABSTRACT ART (Mine) http://nathanbelomy.com
Edited by Fluxburn (02/11/05 08:00 PM)
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: agar plates... [Re: Fluxburn]
#3768604 - 02/11/05 08:24 PM (18 years, 11 months ago) |
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Quote:
Fluxburn said: I squirted some sryinge spores on these and was hoping for the best. I thought myco was only white but I am reading there is white bacteria? I forgot to add that I finished my syringe on this so there was a little extra fuild so it was spread all over the place. I looked at it closey and it looks like mycillum
I think squirting was you're problem here, I guess you've read the thread and have picked up a couple tricks... you can turn your rubbermaid into a glove box for about $10, dryer vent and rubber gloves, the proper application of duct tape and a piece of plexiglass, you'll be set then... arag is pretty fun to work with once you have the environment for it
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Fluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA
Last seen: 13 years, 2 months
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Re: agar plates... [Re: Prisoner#1]
#3768632 - 02/11/05 08:30 PM (18 years, 11 months ago) |
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yah, mine cost more like $30 but I live in a high priced area.
-------------------- ABSTRACT ART (Mine) http://nathanbelomy.com
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