Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineAkira
CosmicConsciousness
Male User Gallery

Registered: 12/30/05
Posts: 2,283
Loc: Hay Un Mundo Mas Alla
Last seen: 11 years, 6 days
Agar Plates
    #5137527 - 01/04/06 02:11 AM (18 years, 28 days ago)

I was wondering if anyone knew where i can purchase agar plates that are ready to use? Cant find any info on were to purchase ready to use agar plates.


--------------------

Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh


Extras: Filter Print Post Top
InvisibleDragonaut

Registered: 06/24/04
Posts: 6,190
Re: Agar Plates [Re: Akira]
    #5137576 - 01/04/06 02:28 AM (18 years, 28 days ago)

~http://www.shroomery.org/sponsors.php
~http://search.ebay.com//search/search.dll?from=R40&satitle=agar+plates


--------------------
:dragon:


Edited by WI_Shroomer_Skater (01/04/06 02:34 AM)


Extras: Filter Print Post Top
Invisibleshymanta
Mad Scientist
 User Gallery

Registered: 01/27/05
Posts: 907
Re: Agar Plates [Re: Akira]
    #5137586 - 01/04/06 02:35 AM (18 years, 28 days ago)

Here you go:
Polystyrene
http://scientificsonline.com/product.asp?cs=p&pn=3081529
Glass
http://scientificsonline.com/product.asp?pn=3081584&bhcd2=1136367451

Took me forever to find these.  They're a really good price.  :cool: Everything else I looked at was way to expensive.

They say "Sterilized prior to packaging, (not for immediate sterile use)".  I got some and just clean them with bleach-water in still air (glove box). :smile:


Extras: Filter Print Post Top
OfflineAkira
CosmicConsciousness
Male User Gallery

Registered: 12/30/05
Posts: 2,283
Loc: Hay Un Mundo Mas Alla
Last seen: 11 years, 6 days
Re: Agar Plates [Re: shymanta]
    #5138465 - 01/04/06 12:11 PM (18 years, 27 days ago)

Im trying to buy some already poured plates. I want to inaculate 24 (1)quart jars, how many agar plates do you think i need to purchase in order to spore all jars?


--------------------

Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh


Edited by GodsEntelechy (01/04/06 12:14 PM)


Extras: Filter Print Post Top
InvisiblePremedman1
Assistant to the insistent
 User Gallery

Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
Trusted Cultivator
Re: Agar Plates [Re: Akira]
    #5138592 - 01/04/06 12:38 PM (18 years, 27 days ago)

That many quarts, you may want to look into a liquid culture tek.
Here's a few  :thumbup:


--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.


Extras: Filter Print Post Top
InvisibleFooMan
 User Gallery

Folding@home Statistics
Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
Trusted Cultivator
Re: Agar Plates [Re: Akira]
    #5138594 - 01/04/06 12:38 PM (18 years, 27 days ago)

Why not just inoculate with a spore syringe? You'll have to wait for the spores to germinate on the agar anyway, so you won't be saving any time by using agar. Look into liquid cultures (LC's) if you are trying to get the most out of your syringe(s).


--------------------

Quick WBS Prep


Extras: Filter Print Post Top
InvisibleFooMan
 User Gallery

Folding@home Statistics
Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
Trusted Cultivator
Re: Agar Plates [Re: Premedman1]
    #5138598 - 01/04/06 12:39 PM (18 years, 27 days ago)

Quote:

Premedman1 said:
That many quarts, you may want to look into a liquid culture tek.
Here's a few  :thumbup:




Beat me to it! :grin: :thumbup:


--------------------

Quick WBS Prep


Extras: Filter Print Post Top
InvisiblePremedman1
Assistant to the insistent
 User Gallery

Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
Trusted Cultivator
Re: Agar Plates [Re: FooMan]
    #5138645 - 01/04/06 12:52 PM (18 years, 27 days ago)

Quote:

FooManShroom said:
Beat me to it! :grin: :thumbup:




All the girls say I'm quick on the trigger :blush:
Seriously, agar is a pain in the ass. All you need is 2 petri dishes and try to isolate a sub-strain, and you'll soon see you can't beat LC. (wow, that sounded gay) :confused:


--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.


Extras: Filter Print Post Top
OfflineAkira
CosmicConsciousness
Male User Gallery

Registered: 12/30/05
Posts: 2,283
Loc: Hay Un Mundo Mas Alla
Last seen: 11 years, 6 days
Re: Agar Plates [Re: FooMan]
    #5138650 - 01/04/06 12:53 PM (18 years, 27 days ago)

Well I wanted to isolate a strain, they told me that by doing so (vs multiple spore syringes) you will get a much healthier and larger yield. And i definately want that so i figured the extra few days was worth it. And im 24 (1)quarts because my trays are pretty deep. I have seven 5 quart trays, so i figured i would fill them up each with 3 quarts of substrate.


--------------------

Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh


Edited by GodsEntelechy (01/04/06 12:55 PM)


Extras: Filter Print Post Top
OfflineAkira
CosmicConsciousness
Male User Gallery

Registered: 12/30/05
Posts: 2,283
Loc: Hay Un Mundo Mas Alla
Last seen: 11 years, 6 days
Re: Agar Plates [Re: Premedman1]
    #5138664 - 01/04/06 12:56 PM (18 years, 27 days ago)

Quote:

Premedman1 said:
Quote:

FooManShroom said:
Beat me to it! :grin: :thumbup:




All the girls say I'm quick on the trigger :blush:
Seriously, agar is a pain in the ass. All you need is 2 petri dishes and try to isolate a sub-strain, and you'll soon see you can't beat LC. (wow, that sounded gay) :confused:



How does that work? I just want to isolate the stupid strain so i can get my high yields!


--------------------

Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh


Extras: Filter Print Post Top
OfflineAkira
CosmicConsciousness
Male User Gallery

Registered: 12/30/05
Posts: 2,283
Loc: Hay Un Mundo Mas Alla
Last seen: 11 years, 6 days
Re: Agar Plates [Re: Akira]
    #5138689 - 01/04/06 01:02 PM (18 years, 27 days ago)

Oh and another reason why i might not want to go into liquid culture, this is my first grow! I already mastered the technique for rye growing in terms of knowledge, now i just need the experience. It would be a pain in the ass to spend another week doing as much research as i did for this method to now have to do it all over again.


--------------------

Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh


Extras: Filter Print Post Top
OfflineWronguy
Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 6 months
Trusted Cultivator
Re: Agar Plates [Re: Akira]
    #5138720 - 01/04/06 01:07 PM (18 years, 27 days ago)

Liquid cultures are extremely easy. Once you have your strain isolated, allow your spores to germinate using a liquid culture. From there you can create many other liquid cultures using one to propagate the other. It doesn't get much easier.


Extras: Filter Print Post Top
OfflineAkira
CosmicConsciousness
Male User Gallery

Registered: 12/30/05
Posts: 2,283
Loc: Hay Un Mundo Mas Alla
Last seen: 11 years, 6 days
Re: Agar Plates [Re: Wronguy]
    #5138750 - 01/04/06 01:12 PM (18 years, 27 days ago)

Theirs not that much info on liquid culture, i rather just stick to rye for right now. So about strain isolation with agar or other........?


--------------------

Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh


Extras: Filter Print Post Top
InvisiblePremedman1
Assistant to the insistent
 User Gallery

Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
Trusted Cultivator
Re: Agar Plates [Re: Akira]
    #5138769 - 01/04/06 01:17 PM (18 years, 27 days ago)

Did you even click the link I gave you earlier? Everything you need to know is there. There's like 8 different LC teks. It sounds like your pretty set on agar, so search. There's a lot about isolation in the advanced mycology section if that tells you anything. Can't explain strain isolation in one post.


--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.


Extras: Filter Print Post Top
InvisibleFooMan
 User Gallery

Folding@home Statistics
Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
Trusted Cultivator
Re: Agar Plates [Re: Akira]
    #5138770 - 01/04/06 01:17 PM (18 years, 27 days ago)

Liquid culture is hardly something that takes too much research. I believe Road Kills recipe calls for:

-4cc's of Karo syrup
-96cc's warm water

Put the karo and water into a jar(s)
PC for about 20-30 minutes
Let Cool
Inoculate with spore syringe
Wait! :grin:

As far as your isolate producing high yields, you'll have to try a few. Read  THIS thread if you haven't already.

You'll get nice flushes from multispore inoculations if done right.


--------------------

Quick WBS Prep


Extras: Filter Print Post Top
OfflineAkira
CosmicConsciousness
Male User Gallery

Registered: 12/30/05
Posts: 2,283
Loc: Hay Un Mundo Mas Alla
Last seen: 11 years, 6 days
Re: Agar Plates [Re: FooMan]
    #5138982 - 01/04/06 02:04 PM (18 years, 27 days ago)

oh man, im just going to do the multi spore method for my first grow, dont want to get myself in a weep of trouble for trying to go all out without prior experience with srooms at all.


--------------------

Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh


Extras: Filter Print Post Top
Invisibleshymanta
Mad Scientist
 User Gallery

Registered: 01/27/05
Posts: 907
Re: Agar Plates [Re: Akira]
    #5140937 - 01/04/06 09:31 PM (18 years, 27 days ago)

Quote:

GodsEntelechy said:
Im trying to buy some already poured plates.




Man, I hardly trust and agar plate not to contam between pouring and full growth. I wouldn't want one handled by a bunch of people and shipped across country.

Pouring isn't hard. All you need is a good recipe http://www.shroomery.org/index.php/par/23367 and still air. Have you read Psilocybin: Magic Mushrooms Grower's Guide? Sounds like you have.

If you want an isolated strain, and are willing to wait... You might inoculate (multi-spore) a PF cake (or grain) and grow that. Take some tissue from the biggest fruits and put it on agar. Let it grow. Now you have an isolated strain that is guaranteed to fruit... and you have some mushies to eat.


Extras: Filter Print Post Top
OfflineAkira
CosmicConsciousness
Male User Gallery

Registered: 12/30/05
Posts: 2,283
Loc: Hay Un Mundo Mas Alla
Last seen: 11 years, 6 days
Re: Agar Plates [Re: shymanta]
    #5141115 - 01/04/06 10:07 PM (18 years, 27 days ago)

YES! ^^ Thats exactly what i will do. Thanks.


--------------------

Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh


Extras: Filter Print Post Top
Invisibleshymanta
Mad Scientist
 User Gallery

Registered: 01/27/05
Posts: 907
Re: Agar Plates [Re: Akira]
    #5176251 - 01/13/06 11:17 AM (18 years, 18 days ago)

GodsEntelechy,

If you're still looking for pre-sterilized agar, I found a some at Myco Supply. They might do okay even after shipping across country.
http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=PPSM01
Plus their a Shroomery Sponsor.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Re: mold in agar plate Anonymous 12,180 3 12/04/99 12:35 AM
by Anonymous
* Whats with my agar plates? Frankenstein 8,594 8 09/08/22 03:46 PM
by schpat
* What to do with 1st agar plate fromthemoon 1,382 3 09/07/06 03:20 PM
by monstermitch
* Agar Plates Start to Finish? shymanta 2,007 7 01/01/06 11:23 PM
by Corporal Kielbasa
* Many sub-strains in a multispore LC? Similar to different types of myc on an agar plate? blackout 1,741 5 07/06/05 12:22 PM
by Roadkill
* agar plate jellyfish 576 4 01/05/07 01:02 PM
by Blutjager
* How many mycelium syringes from one agar plate? BBShrizz 4,880 3 05/10/01 02:10 AM
by ISaToriBluEI
* moisture build up on agar plates Pie 1,667 1 05/31/05 08:02 PM
by waixingren

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,920 topic views. 16 members, 130 guests and 16 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.005 seconds on 12 queries.