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Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Petri Dish, Vermiculite

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Offlinefoxxybrown
Foxxybrown
Registered: 08/28/03
Posts: 9
Loc: alberta,canada
Last seen: 13 years, 9 months
Agar to Rice flour substrates
    #1865922 - 08/30/03 03:58 PM (13 years, 10 months ago)

I have a few very successful agar mycelium growths and wonder if they can be transported to the rice flour jars? I haven't come across anyone who has done this method, but wonder if anyone in the forum has tried this or knows if this is possible with successful results?


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Invisiblekoraks
Registered: 06/02/03
Posts: 25,210
Re: Agar to Rice flour substrates [Re: foxxybrown]
    #1865942 - 08/30/03 04:07 PM (13 years, 10 months ago)

Why not? I'd say go ahead. I'm surprised that no-one has ever written about this before, but I think it should work just fine!


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Agar to Rice flour substrates [Re: foxxybrown]
    #1865950 - 08/30/03 04:13 PM (13 years, 10 months ago)

If you are talking about agar wedge transfers to PF tek jars then no, the verm layer would be a problem.

You could omit the verm layer and filter the jars with a disk or Tyvek. You would have to perform the transfer in a sterile environment to avoid contamination. Be sure to leave some space at the top of the jar so you can shake it.

I'm not sure how well a BRF/verm substrate would shake, but it might work.


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Offlineeggy
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Registered: 05/20/03
Posts: 21
Loc: south africa
Last seen: 1 year, 6 months
Re: Agar to Rice flour substrates [Re: dog]
    #1870527 - 09/01/03 07:00 AM (13 years, 10 months ago)

your agar in petri dishes? pour some sterile water in there, scrape some mycelium off the agar with a sterile scalpel, suck some of the mixture up into a sterile syringe. use THAT to innoculate your jars... BUT, maybe dilute what you get and make a couple more syringes first...
nope: never tried it, have read that you can do it...
let us know...


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Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Petri Dish, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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