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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Shop: Myyco.com APE Liquid Culture For Sale   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #25760858 -

Those are beautiful plates.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dephect]
    #25761073 -

Anyone know for sure what can cause agar to not set? I had a couple batches that didn’t solidify. I’ve heard ph can play a part in that but it seemed so nuts that 2 in the last 50 batches didn’t set up. After last night’s fail I did another batch that I just cooked hotter and longer while mixing and it was fine.


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"Once your spawn jars are fully colonized, you can scratch your butt while you inoculate the coir if you want."-RR

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dephect]
    #25761077 -

I’ve been trying to wait these out but it’s bugging me.  There is a bit of condensation but think the pic is clear enough.  This is APE.  My first time with it.  Does it usually look so grainy?





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Re: AGAR ENVY! (Anything and All things agar!) [Re: sporealchemy]
    #25761190 -

sporealchemy said:
Anyone know for sure what can cause agar to not set? I had a couple batches that didn’t solidify. I’ve heard ph can play a part in that but it seemed so nuts that 2 in the last 50 batches didn’t set up. After last night’s fail I did another batch that I just cooked hotter and longer while mixing and it was fine.


Do you have too much agar powder? Less makes it harder and more makes it softer I believe

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Re: AGAR ENVY! (Anything and All things agar!) [Re: woofwoof]
    #25761253 -

I use 7g agar, 7g MEA, 500ml water. It went runny twice prepped with red food dye. It worked like it usually does after I didn’t rush the process and used blue but I don’t think the different colors have seriously varying ph values or anything. Only happened once before but I may have been rushing each time. Seemed weird bc it always sets up but last night I was cooking agar while setting the heat for pc grains and also spawning tubs lol.

Your ape plate doesn’t look bad. Albino varieties in particular can look a little funny.


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"Once your spawn jars are fully colonized, you can scratch your butt while you inoculate the coir if you want."-RR

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sporealchemy]
    #25761435 -

Photoshoot!:



2 tissue samples taken from the same fruit from RW clone #1 (hence 1.1&1.2):



Will be taking transfers from these guys after dinner... Grabbing the 5 biggest rhizomorphic ropes from 11-1  in the PE6 clone culture I pulled a transfer from out of cold storage since early june... taking 5 transfers from RW between 5-9, and ideally 5 from red boy from 7,10,11,2, and 3... second picture is APE T1 going rhizo I'll be getting 5 from between 1-6, and the last showing a close-up of the red boy with some crazy sectoring:



3 T1 transfers from a clone, from RW clone #2, looking clean, may go to grain:



Cultures that will be split to 2 jars a peice in 3 days  (boil oats tmrw, let dry overnight, pc the following night, and A2G drops Saturday morning) will be comparing tomentose and rhizomorphic PE6 cultures:



2 AA+ plates (T3 and T4) knotting up:



PE6 MS tub >clone>agar pin clone >agar pin clone, and the first 2 pins from it... so 2 3rd gen agar pin clones:



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🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: woofwoof]
    #25761442 -

woofwoof said:
sporealchemy said:
Anyone know for sure what can cause agar to not set? I had a couple batches that didn’t solidify. I’ve heard ph can play a part in that but it seemed so nuts that 2 in the last 50 batches didn’t set up. After last night’s fail I did another batch that I just cooked hotter and longer while mixing and it was fine.


Do you have too much agar powder? Less makes it harder and more makes it softer I believe


Exactly the opposite, more makes it harder and less softer. 9g agar to 500ml is my go to, with spores its 7g to 500ml, they like a little more water.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MH5109]
    #25761453 -

Thanks Sorealchemy!  It wasn’t changing with the transfers so I figure I better ask.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25761661 -

ShaperDreaming said:
So... I am noticing that at about the 1 month point that my agar goes color-less on plates.

Pet theory: The myc eats all the sugars from the food coloring
Likely theory: it just fades from light exposure.

Anyone know for sure?


Thats funny because i was going to also make a comment about the red food coloring completely gone from some of my agar plates.


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Trade list-
http://www.shroomery.org/forums/showflat.php/Number/21740145

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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup]
    #25761678 -

I only colored a few times... found it to actually make it more difficult for me to spot any contaminants


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🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25761729 -

:whathesaid:

Never found any use for coloring!


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My improved magnetic stirrer
Breeding mushroom strains
Potato-Honey-Yeast-Agar Tek

My Enoki grow tek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sporealchemy]
    #25761732 -

sporealchemy said:
Anyone know for sure what can cause agar to not set? I had a couple batches that didn’t solidify. I’ve heard ph can play a part in that but it seemed so nuts that 2 in the last 50 batches didn’t set up. After last night’s fail I did another batch that I just cooked hotter and longer while mixing and it was fine.


Did you have any hard sediment? Perhaps it was not mixed well?
10g/500ml is the usual recipe, at 7 you would be on the soft side. Which recipe are you following?


--------------------
My improved magnetic stirrer
Breeding mushroom strains
Potato-Honey-Yeast-Agar Tek

My Enoki grow tek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: denger]
    #25761917 -

Is there alcohol in your dye?


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Never give up, never surrender.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: denger]
    #25761932 -

Iv heard of people putting the agar/nutrients in the flask and then dumping h2o on it, not mixing it at all and PCing it with success. For real the only reason you get soft agar is you don't have enough agar in your mix, maybe they were a little lite. If you mix 9g agar to 500ml h2o guaranteed you get firm agar.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MH5109]
    #25762075 -

Did you have remnants in the vessel? Like left overs that set before pouring?... I put my dry ingredients(3g agar and 6.5g LME) into the vessel, put roughly 120ml of hot water from the tap and swirl it quickly before the malt clumps, then fill it the rest to 500ml with hot tap, pc directly... I still get sediment, but not bad


--------------------
🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25762082 -

I always cook mine for 5min or so, it would be alot easier to mix it in the vessel, I always have sediment in the agar, never worried about it.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MH5109]
    #25762100 -

Doesn't bother me either :shrug:


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🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25762789 -

Caps, loving your info man!  You've been a big help to all.  Quick question, are you working in a SAB?  I have an opportunity to head to a lab with plates and agar in a flow/fume hood workspace.  Do you think pouring and transporting to my home mini lab presents an issue?  I'd like to take the opportunity to do some transfers and pours in front of the hood but can't leave em sitting there once done and set up.  My SAB is working but I am having contamination issues in places.
HH


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It looks a lot more like it does now than it did...


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25763019 -

Caps McGee said:
I only colored a few times... found it to actually make it more difficult for me to spot any contaminants


The reason I'm doing it (I didn't start doing it, I started dye free) was because I was having a hell of a time finding the vanguard of good myc on the surfaces I work on.

My tables are all woodgrain, which makes finding the edge of growth pretty challenging. So I started to add color to differentiate from the texture of grain-y-ness better.

If I had a proper lab and shit I'd probably not be using food color.


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My Shoebox Factory Beginner's Tek | Shoebox Assembly Tek | Methodology/teks I use | How I Store Bulk Items

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25763045 -

My tables are all woodgrain, which makes finding the edge of growth pretty challenging. So I started to add color to differentiate from the texture of grain-y-ness better.

If I had a proper lab and shit I'd probably not be using food color.


I have a roll of paper towel handy at all times and set plates on that...I too have woodgrain workbench made from a solid core commercial door.  The paper towel is white as pure snow and helps with lighting as well.


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It looks a lot more like it does now than it did...


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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