woofwoof said:

What is difference between strain isolation and cloning?? Will strain isolation provide equal genetics like cloning does??

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RogerRabbit said:

Cloning has been used for years.  It just isn't strain isolation.  In fact, many strains can be incorporated into a single fruitbody when multispore inoculation is used, especially on the first flush.  When isolating strains, usually it takes several transfers to begin to even see sectoring, and a few more to get down to individual strains.  I see where you're coming from, but didn't want the new folks to get confused.  They're two totally different processes.
RR

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Caps McGee said:

Take your largest,fastest finishing fruit from clusters for clones... use the weight of the smallest of those as your test dose, as you won't be able to bioassay anymore of that particular fruit than you have... i.e., if you have 2- 2g fruit, and one that's only 1.5g, dose at 1.5g to get a more accurate comparison... you can't take 2g of a fruit that only weighs 1.5g

I pour my agar soft as hell so hydration isn't necessary... the print you'll receive in a few days was printed last Tuesday, so shouldn't be any issue anyway... only use a small amount and you should be able to stretch the print to printing your own in masses

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ShaperDreaming said:

woofwoof said:

What is difference between strain isolation and cloning?? Will strain isolation provide equal genetics like cloning does??

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Strain isolating is v difficult from what I understand. What people tend to throw around, around here as "strain isolation" is still pretty unisolated. It's effectively trying to narrow the different strains within a culture. So, if you put something on agar and you grab a tiny tendril of mycelium and put it on to new agar, you're "isolating" further. It seems likely impossible for the amateur mycologist to actually isolate a single strain. This isolation can make it so that your tubs of bulk fruit at approx the same time, look similar in shape and form, and have similar potency.

Cloning is putting fruit to a substrate where it breaks back down into mycelium that you can then do strain isolation from.

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Quirkmeister92 said:

Good surface conditions...

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Caps McGee said:

You could isolate a shitty strain... starting with a clone guarantees at mimimum, a culture that will fruit... you can do further isolation, but I've found original clone cultures outperform 11 of 12 isolates derived from that culture... a clone culture, though still containing multiple strains, is a monoculture, as it works together to perform as a single culture... but without

Quirkmeister92 said:

Good surface conditions...

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You won't find what you're looking for in any event

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Caps McGee said:

You'll get uniformity with isolation,  whether from MS on agar, or cloning ... just may end up with a culture that uniformly sucks, lol... at least with clones, you know it'll fruit

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woofwoof said:

ShaperDreaming said:

woofwoof said:

What is difference between strain isolation and cloning?? Will strain isolation provide equal genetics like cloning does??

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Strain isolating is v difficult from what I understand. What people tend to throw around, around here as "strain isolation" is still pretty unisolated. It's effectively trying to narrow the different strains within a culture. So, if you put something on agar and you grab a tiny tendril of mycelium and put it on to new agar, you're "isolating" further. It seems likely impossible for the amateur mycologist to actually isolate a single strain. This isolation can make it so that your tubs of bulk fruit at approx the same time, look similar in shape and form, and have similar potency.

Cloning is putting fruit to a substrate where it breaks back down into mycelium that you can then do strain isolation from.

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I want uniform flushes and fruit size, etc. This can be done with cloning tissue or will I still need to isolate further??

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