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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Black Swirls in LC
    #18809275 -

Hey guys, I made this grain LC off a jar that colonized to 100% and did not smell funky after breaking and shaking. Im not sure what this is, and Ive never had it show up on any previous LC's Ive made (that were all successful), so Im coming for advice.

I used the search function and came across a thread and someone said black dots can be common in LCs. Idk how true that is. How do these look to you guys? Is the black swirling normal or am I facing a contam? Two out of three LCs have the black swirls.

The black only swirls around the mycelium itself. The LC's are about a month old, didnt store em in the fridge, just in a dark cool place to grow out.





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Re: Black Swirls in LC [Re: Nimpo]
    #18809304 -

Nimpo said:
How do these look to you guys? Is the black swirling normal or am I facing a contam? Two out of three LCs have the black swirls.

The black only swirls around the mycelium itself.


There is no way to visually tell if an LC is good or not.

Test jars must be shot up and grown out.


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: Black Swirls in LC [Re: Nimpo]
    #18809305 -

Could be cotton web or spider web (whichever it's called?) sporulating, could be mushroom spores, could just be the mushroom mycilium 'harvesting' minerals. Might even be trich spores lookg black, could even be a trick of the light for that matter. This is why they say LC's are unreliable until you use them, you never know what it is.

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Re: Black Swirls in LC [Re: krypto2000]
    #18809355 -

Ive got a bunch of bags to shoot up as test subjects, but Im here for some first hand accounts (because I havent run into this and Ive made over 12 LCs) just in case someone knows whats up before I decide to wreck these bags which could be used for spawn instead of test dummies.

If a TC chimes in and confirms a contam, then I'll know to not even bother testing.

I just dont know if it is, because it could be anything (unlikely mushroom spores since it came from grain). However, the black is new, cause it wasnt there a week ago, and that has me a little concerned.

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Re: Black Swirls in LC [Re: Nimpo]
    #18809367 -

Nimpo said:
If a TC chimes in and confirms a contam, then I'll know to not even bother testing.


They are going to tell you the same thing.....


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: Black Swirls in LC [Re: PussyFart]
    #18809379 -

Notahacker420 said:
Nimpo said:
If a TC chimes in and confirms a contam, then I'll know to not even bother testing.


They are going to tell you the same thing.....


Some LCs can clearly show contams, which is what Im getting at here.

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Re: Black Swirls in LC [Re: Nimpo]
    #18809398 -

Right, but in order to be 100% certain, shoot up some test jars bro....

The TCs could be wrong and you could toss out a perfectly good LC...

Would that be worth it to you?

Test it yourself and see.

To me, I say it looks contaminated, but that is just my opinion, as I do not bother with LCs for this very reason.

The very fact that there is uncertainty just doesn't settle right with me...

Just test it....


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

Edited by PussyFart (09/06/13 09:05 PM)

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Re: Black Swirls in LC [Re: Nimpo]
    #18809399 -

Personally I would toss it and start over, but there really is no way to know. There are minerals that get dissolved in the water (or at least are so tiny you can't see them initially), but since mushrooms like to eat literally everything it could be that the minerals are simply becoming visible since they're grouped together and are beginning to form a crystalline structure which catches, and thus reflects, the light. That is likely some mineral rich water however if that is the case, but depending on your sugar source it could simply be the minerals from that. Still wait for others, but I really doubt you'll get a conclusive answer here, this is simply why we don't trust LC's.

You said it came from grain, did you do RR's method to make it? Are you sure the grain jar was good before hand? In the future if you're not sure just do his method and then use the grain as spawn or even a g2g transfer (so you have enough for bulk with just that original spawn) in order to test it. You'll always have contaminant spores present no matter what, some will always survive the pressure cooker (at least endospores), and during the process it's near impossible some don't get introduced, but you can at least judge roughly how your LC will perform based on how the jar you started it from performs.

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Re: Black Swirls in LC [Re: krypto2000]
    #18809451 -

I didnt use RR's method, I actually created my own bag LC tek which has been 100% successful so far, but this might be my first bunk.

Alright Im gonna shoot two bags up right now, 1 with a LC sporting black swirls, and the second will contain a LC from the same batch, but shows no black swirls.

Ill rebump this thread (for the sake of science and other posters) in a few days with more information.

I suspect a contam, but we will find out soon.

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Re: Black Swirls in LC [Re: Nimpo]
    #18809579 -

It looks contaminated to me as well...

Why do you use LCs instead of agar?

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Re: Black Swirls in LC [Re: TrentBoyett]
    #18809633 -

mjmihalov said:
It looks contaminated to me as well...

Why do you use LCs instead of agar?


I normally make a spawn bag and shoot it up with a strain of spores of my choosing that I havent grown. Once that hits 100% (after a mix at 30%), I can use the filter patch to better smell whats going on inside than a grain jars patch. Once I confirm that all smells right, I shoot the same bag up with sterilized water through a self healing port, mix everything (much easier in a bag than in a jar), then pull the mycelium solution back up into other sterile syringes via the self healing port. I then use that one syringe to make 8 other spawn bags while the grain that was used to make the LC is thrown into some poop to fruit as my test subject.

I dont have the sterility practice desire to start agar work and g2g, or buying the mats to make em (dishes, agar, glass jars). I tried agar a few times but just couldnt get into it (and they all failed, which was alright cause watching the bacteria colonies grow was cool). I have over 45 lbs of grain and 150+ bags so this method works for me (mostly, this might be my first slip up), especially with how lazy I am and the success Ive had so far.

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Re: Black Swirls in LC [Re: Nimpo]
    #18850813 -

Bumping for science with an update.

Its been about 9 days since injection, both bags sitting at 55ish% with no signs of contams.

Broke up and shook a bag, mixed, sniffed the patch, and it smelled great. :thumbup: So far, everything is solid.

Ill update again once both hit 100% and I get my nose in there

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures

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