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Amazon Shop: ½ Pint Jars, Agar, Malt Extract, Wild Bird Seed

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OfflineChastity_Belt
Unholy livingdead

Registered: 06/03/05
Posts: 188
Last seen: 12 years, 1 month
LC jar lids...attaching tubing sections with RTV HT stuff...help!
    #4273962 - 06/09/05 12:18 AM (12 years, 1 month ago)

    So I've tried to attach latex tubing (bungee cord surgical type stuff) with the RTV high-temp gasket maker stuff.  I'm having some problems, though, as the gasket goo doesn't seem to bond well with the latex tubing or the jar lid.  Just tried to do some jars of LC up and the goo came out looking like it wasn't sealed still. 
    Questions, then:
    I have a 7 quart PC that just fits quart jars; with tubing on top this PC lid presses down and bends the tubing while it cooks.  Is this stress causing the problem? 
    Is there a way to make the bond stronger between these materials and the gasket maker goo? 
    Is there a better type of tubing (e.g. catheter tubing) that would be better as its diameter is smaller and thus is less resistant to the PC lid bending and also is easier to fix in with the gasket maker because it's smaller than surgical tubing? 

    This is sort of for Agar, but, anyone who can help, please do.  :laugh:
    I'm f'ing sick of this shit.  :mad2:


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InvisibleHolydiver
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: Chastity_Belt]
    #4273982 - 06/09/05 12:21 AM (12 years, 1 month ago)

You guys make simple things ultra complicated, and it kills me. (not you personally) All you need for a good liquid culture lid is a 1/4" drilled hole stuffed with polyfill, and another hole big enough for a 16-20 gauge needle. Insert 1-2cc spores, cover with tape. Once colonized, wipe tape with alcohol and draw liquid culture right into sterilized syringe.



--------------------
To find a place to live between the negatives and positives.


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OfflineChastity_Belt
Unholy livingdead

Registered: 06/03/05
Posts: 188
Last seen: 12 years, 1 month
Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: Holydiver]
    #4273990 - 06/09/05 12:23 AM (12 years, 1 month ago)

It's the swishing that's a problem...I don't want to have a filter that can get soaked.  Maybe I'll just have to be careful about that and use some tyvek as it appears I'M A FUCKING RETARD with tubing.  :smirk: 
  Thanks, dude.  :cool:


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InvisibleHolydiver
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: Chastity_Belt]
    #4273994 - 06/09/05 12:26 AM (12 years, 1 month ago)

Use a shouldered jar, and a gentle swirl shouldn't cause you any problems. I never shake a LC, just a swirl once or twice during the cycle is all you need.



--------------------
To find a place to live between the negatives and positives.


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OfflineChastity_Belt
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Registered: 06/03/05
Posts: 188
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: Holydiver]
    #4274020 - 06/09/05 12:33 AM (12 years, 1 month ago)

I think I may have to do this until I get a bigger PC.  :cheers:


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Invisibleagar
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: Chastity_Belt]
    #4274190 - 06/09/05 01:11 AM (12 years, 1 month ago)

It may be the LATEX TUBING... is the problem.

I use rubber tubing (urine cathiter type) & have never had a problem with RTV gasket material sticking to the tubibg, or lid.


--------------------


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OfflineChastity_Belt
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Posts: 188
Last seen: 12 years, 1 month
Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: agar]
    #4274343 - 06/09/05 02:03 AM (12 years, 1 month ago)

Thanks, G money.  :wink:

I may have to hit up my local med supply shop and just see if that works better.  :cool:


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OfflineHippieChick
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: Chastity_Belt]
    #4274365 - 06/09/05 02:17 AM (12 years, 1 month ago)

i used Agar's advice and used windshield washer wiper tubing and didn't have any problems,



using the same sealant you are.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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OfflineChastity_Belt
Unholy livingdead

Registered: 06/03/05
Posts: 188
Last seen: 12 years, 1 month
Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: HippieChick]
    #4274381 - 06/09/05 02:24 AM (12 years, 1 month ago)

How much did that run you?  And can you get it at any autoparts store?  (Like Autozone, whatever?).  Thanks.  :laugh:


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InvisibleThumpaCap
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Registered: 02/19/05
Posts: 568
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: HippieChick]
    #4274384 - 06/09/05 02:25 AM (12 years, 1 month ago)

is this the best way to start off pan cyan?... to start a liquid culture with spore syringe first?.. or is it just as good to go ahead and squirt the spores on WBS?... and what liquid media recipe is best for pan cyan/??


--------------------
:bongload: Look into my heyes !! :bongload:


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OfflineHippieChick
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: Chastity_Belt]
    #4274391 - 06/09/05 02:28 AM (12 years, 1 month ago)

I think I got a few feet of it for $1.59. Should be able to get it at any auto part store. I got mine at AutoZone.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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OfflineChastity_Belt
Unholy livingdead

Registered: 06/03/05
Posts: 188
Last seen: 12 years, 1 month
Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: HippieChick]
    #4274394 - 06/09/05 02:31 AM (12 years, 1 month ago)

w00t!  Thanks.  I'll give it a shot.  Far cheaper than the latex tubing I was using, anyway.  :smile:


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OfflineHippieChick
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: ThumpaCap]
    #4274439 - 06/09/05 02:49 AM (12 years, 1 month ago)

Not sure what is best for pan cyan/, if it would require anything special, but I like to use a tablespoon each of light malt extract and dextrose, $6 for both and enough to last a lifetime,



per quart of distilled water. PC'ed for 20 minutes at 15 psi.

Good Luck.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Invisiblefinding_self
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Registered: 05/15/05
Posts: 489
easy [Re: HippieChick]
    #4274447 - 06/09/05 02:51 AM (12 years, 1 month ago)

easy


Edited by finding_self (04/18/06 10:36 PM)


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Invisibleagar
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: finding_self]
    #4274452 - 06/09/05 02:53 AM (12 years, 1 month ago)

Almost any home brew beer & wine making supply store has it. Look in your local phone book.  :thumbup:

PS: It works for Pan's C.


--------------------


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OfflineHippieChick
Chicks can do it too!
Female User Gallery

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Posts: 5,958
Loc: Midwest
Last seen: 23 days, 9 hours
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: finding_self]
    #4274460 - 06/09/05 02:54 AM (12 years, 1 month ago)

Check stores that sell supplies for home beer brewing and wine making.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Invisibleagar
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: HippieChick]
    #4274467 - 06/09/05 02:56 AM (12 years, 1 month ago)

I think there is an echo in this thread......LOL :wink: :heart:


--------------------


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OfflineHippieChick
Chicks can do it too!
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Posts: 5,958
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: agar]
    #4274477 - 06/09/05 03:00 AM (12 years, 1 month ago)

I was gonna say, " Damn, you know where I got my info from,lmao. " :heart:

Thanks for that, and all the other advice. :heart: :grin:

:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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OfflineChastity_Belt
Unholy livingdead

Registered: 06/03/05
Posts: 188
Last seen: 12 years, 1 month
Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: agar]
    #4274479 - 06/09/05 03:01 AM (12 years, 1 month ago)

I got that same stuff.  Must say...best LC I've seen from that combo.  One tablespoon each to a full quart of warm water, mix, pc 20 minutes, strain through a coffee filter, fill quart jars to about 1/2 ~ 3/5 full, pc 20 again.  Very nice myc. in a week or two.  :sun:  :thumbup: Cheers agar.  :laugh:


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OfflineHippieChick
Chicks can do it too!
Female User Gallery

Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 23 days, 9 hours
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Re: LC jar lids...attaching tubing sections with RTV HT stuff...help! [Re: Chastity_Belt]
    #4274494 - 06/09/05 03:05 AM (12 years, 1 month ago)

I'm glad you told em about filtering. I forgot.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:

So did you Agar,lol.


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Amazon Shop: ½ Pint Jars, Agar, Malt Extract, Wild Bird Seed

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