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Amazon Shop: ½ Pint Jars, 10cc Syringe, Brown Rice Flour

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OfflineSquidfingers
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Registered: 08/27/04
Posts: 33
Last seen: 11 years, 10 months
Simple LC questions
    #4478569 - 07/31/05 10:38 PM (11 years, 11 months ago)

Well I have 6 liquid cultures (3 B+ and 3 GT) that I've had in my fruiting chamber for 3 weeks with my BRF jars at 83 degrees. Only 2 of the B+ showed any good growth and 1 of the jars has some specks that look like myc but they're in little balls and don't look that heathy. Soooo...

1). I see that the myc in the jar is a light brown now with a dark brown speck or two. Does the color really matter?

2). I think they only really needed to be in the incubator for 7-10 days but I didn't get a chance to throw them in the fridge. Will the extra week or so in the heat hurt them?

3). When I make my syringes do I only suck up the strands of myc in the syringe or does all the liquid have spores? If so how much myc has to be sucked up per syringe?


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OfflineHotnuts
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Registered: 02/26/05
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Re: Simple LC questions [Re: Squidfingers]
    #4480240 - 08/01/05 09:07 AM (11 years, 11 months ago)

Quote:

Squidfingers said:
Well I have 6 liquid cultures (3 B+ and 3 GT) that I've had in my fruiting chamber for 3 weeks with my BRF jars at 83 degrees. Only 2 of the B+ showed any good growth and 1 of the jars has some specks that look like myc but they're in little balls and don't look that heathy. Soooo...

1). I see that the myc in the jar is a light brown now with a dark brown speck or two. Does the color really matter?

2). I think they only really needed to be in the incubator for 7-10 days but I didn't get a chance to throw them in the fridge. Will the extra week or so in the heat hurt them?

3). When I make my syringes do I only suck up the strands of myc in the syringe or does all the liquid have spores? If so how much myc has to be sucked up per syringe?




This is what your probably talking about right? Those specks are the spores that have germinated. The culture will be fine in the incubator, room temp, or the fridge. When you want to aspirate some of the lc, make sure you swirl the jar some to break up the tissue in the jar. Then you'll want to get as much tissue as you can into the syringe. If you draw up 10cc's of LC with some tissue, you can then draw up some air into the syringe (while in your gloove box) so you can shake up the syringe to evenly disperse tissue throughout the syringe.


Edited by hotnutz (08/01/05 09:09 AM)


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OfflineSilverr
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Registered: 06/10/05
Posts: 95
Last seen: 7 years, 11 months
Re: Simple LC questions [Re: Squidfingers]
    #4480899 - 08/01/05 02:49 PM (11 years, 11 months ago)

My .02

Here is a pic of my Burma LC, I have recently Inocc. BRF jars with it and despite all the freakin balls in their, it has worked great so far. Myc growth is visible after day 2 of inocc. I think you should be fine.











GOOD LUCK  :irishtoast:


--------------------
"The only easy day was yesterday"


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OfflineSquidfingers
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Registered: 08/27/04
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Last seen: 11 years, 10 months
Re: Simple LC questions [Re: Squidfingers]
    #4481148 - 08/01/05 03:56 PM (11 years, 11 months ago)

:thumbup: That picture on the far left is exactly what I'm talking about hotnutz. I'm also not sure how many syringes an LC can make. I guess I have a few blobs that size in there, so I'm thinking two with what I have. Thanks for the help you guys.

Also I don't know where to buy some good cheap syringes, scat posted a good link not long ago but I can't find it.


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OfflineSilverr
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Registered: 06/10/05
Posts: 95
Last seen: 7 years, 11 months
Re: Simple LC questions [Re: Squidfingers]
    #4481546 - 08/01/05 05:45 PM (11 years, 11 months ago)

<"Also I don't know where to buy some good cheap syringes, scat posted a good link not long ago but I can't find it. ">

Here ya go bro.

Getpinz.com

PEACE


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"The only easy day was yesterday"


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Offlineshroomballa
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Registered: 03/26/06
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Loc: Montana
Last seen: 10 years, 18 days
Re: Simple LC questions [Re: Silverr]
    #5485038 - 04/06/06 01:12 AM (11 years, 3 months ago)

For syringes, hit up your local pharmacy. When they ask what you need them for, tell them epoxy...


--------------------
Anything I say is fictional. Duh.


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Amazon Shop: ½ Pint Jars, 10cc Syringe, Brown Rice Flour

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