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Blue Helix
bold hand


Registered: 02/02/03
Posts: 1,565
Last seen: 6 months, 17 days
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Re: Far into the LC Myc Matrix [Re: agar]
#4220568 - 05/25/05 10:21 PM (18 years, 8 months ago) |
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AWESOME! Thanks. I love LCs!
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Interested
Stranger
Registered: 11/30/04
Posts: 45
Last seen: 9 years, 7 months
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Re: Far into the LC Myc Matrix [Re: agar]
#4220591 - 05/25/05 10:26 PM (18 years, 8 months ago) |
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Quote:
It would be interesting to cut an inch thick open cell sponge to fit snugly inside the liquids surface in a quart jar of LC, as a platform for primordia to form on.
I've already tried this, in fact! Alas, mycelium growth wasn't near enough to begin pinning, or even enough to really determine whether it had penetrated the sponge. Hopefully your air exchange technique will prove the missing piece of the puzzle.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Far into the LC Myc Matrix [Re: Interested]
#4220741 - 05/25/05 10:56 PM (18 years, 8 months ago) |
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Quote:
Interested said:
Quote:
It would be interesting to cut an inch thick open cell sponge to fit snugly inside the liquids surface in a quart jar of LC, as a platform for primordia to form on.
I've already tried this, in fact! Alas, mycelium growth wasn't near enough to begin pinning, or even enough to really determine whether it had penetrated the sponge. Hopefully your air exchange technique will prove the missing piece of the puzzle.
I think you will need a magnetic stir plate for optimal tests.
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Interested
Stranger
Registered: 11/30/04
Posts: 45
Last seen: 9 years, 7 months
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Re: Far into the LC Myc Matrix [Re: agar]
#4220967 - 05/26/05 12:16 AM (18 years, 8 months ago) |
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Quote:
I think you will need a magnetic stir plate for optimal tests.
Yep, I have one. Were you stirring constantly, or once or twice a day? Can you make a rough estimate of the RPM you were using? Obviously too high, particularly toward the end, would cause mycelium fragmentation detrimental to fruiting (although obviously beneficial for someone looking to make an LC syringe).
Also, thanks for your help. I hadn't expected the luxury of having this much prior work done for me. It's reassuring to think I won't be starting from scratch.
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Aeolus1369
Dr. Seahorse


Registered: 05/20/02
Posts: 367
Last seen: 14 years, 11 months
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Re: Far into the LC Myc Matrix [Re: agar]
#4222741 - 05/26/05 01:36 PM (18 years, 8 months ago) |
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Awesome work as always!
What's your LC recipe by the by?
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