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Invisibleagar
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Far into the LC Myc Matrix
    #4211529 - 05/23/05 09:18 PM (15 years, 5 months ago)


LC MYC MATRIX SPINNING :eek:

LC STILL MATRIX  :tongue2:


Vacutainer



Life is GOOD. The GOOD life is better.


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InvisibleTheStudent
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Re: Far into the LC Myc Matrix [Re: agar]
    #4211565 - 05/23/05 09:29 PM (15 years, 5 months ago)

well....those shroomZ sure do look delicious :oogle:


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Invisiblebackupwards
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Re: Far into the LC Myc Matrix [Re: TheStudent]
    #4211597 - 05/23/05 09:38 PM (15 years, 5 months ago)

and purpose?


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Invisibleagar
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Re: Far into the LC Myc Matrix [Re: backupwards]
    #4211633 - 05/23/05 09:47 PM (15 years, 5 months ago)

Quote:

backupwards said:and purpose?




Magnetic stir plate & stir bar combo stirs the LC at very high speed, thus adding gas/oxegen to the mix & cutting the myc into very tiny strands, which can be asperated out of the culture with ease.

The cultures were generated from a live tissue isolate from an optimal speciman. In other words cloned into a liquid culture.

Thick healthy LC cultures used an an inoculate, give you VERY rapid colonization. Clones most often exhibit the same traits as the parent.



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InvisibleMrMaddHatter
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Re: Far into the LC Myc Matrix [Re: agar]
    #4211638 - 05/23/05 09:48 PM (15 years, 5 months ago)

Quote:

agar said:
Life is GOOD. The GOOD life is better.



:grin: :thumbup: :mushroom2:


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Offlinegrow
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Re: Far into the LC Myc Matrix [Re: agar]
    #4211641 - 05/23/05 09:49 PM (15 years, 5 months ago)

not gonna bother


Edited by grow (05/23/05 09:50 PM)


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InvisibleTheStudent
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Re: Far into the LC Myc Matrix [Re: agar]
    #4211645 - 05/23/05 09:50 PM (15 years, 5 months ago)

Quote:

agar said:




Dear god.....I think I'm in love :eek:


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Invisiblebackupwards
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Re: Far into the LC Myc Matrix [Re: agar]
    #4211648 - 05/23/05 09:51 PM (15 years, 5 months ago)

yes understand the lc. just wasn't sure what you were trying to show. wow that is some amazing cultures you have there. so the pics are the ones you cloned from to make the culture? haven't read up enough on cloning shrooms, but there are others that i have great luck with. the stir plate really does cut the myc up, just a regular bar stir stick?


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Invisiblebackupwards
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Re: Far into the LC Myc Matrix [Re: agar]
    #4211655 - 05/23/05 09:55 PM (15 years, 5 months ago)

Magnetic stir plate & stir bar combo stirs the LC at very high speed, thus adding gas/oxegen to the mix & cutting the myc into very tiny strands, which can be asperated out of the culture with ease.
-------------------------------------------------------------------------------------------------------
The cultures were generated from a live tissue isolate from an optimal speciman. In other words cloned into a liquid culture.

Clones most often exhibit the same traits as the parent.
____________________________________________________________
So as in taking a clone of a mary (100% genetic traits) mushrooms are not the same in that instance? They are not always 100% a genetic match to the parent?


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Invisibleagar
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Re: Far into the LC Myc Matrix [Re: grow]
    #4211663 - 05/23/05 09:58 PM (15 years, 5 months ago)

>>>>>>useless<<<<<<<< :rolleyes:

So is h/poo.... if you don't know optimal methods to put it to use.  :tongue:


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Offlinegrow
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Re: Far into the LC Myc Matrix [Re: backupwards]
    #4211667 - 05/23/05 09:58 PM (15 years, 5 months ago)

that's right


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Invisibleagar
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Re: Far into the LC Myc Matrix [Re: backupwards]
    #4211677 - 05/23/05 10:01 PM (15 years, 5 months ago)

If overall conditions vary, so will the results. So, no (IMHO), they do not always match 100%. But, the odds are better - that they will.


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Invisiblebackupwards
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Re: Far into the LC Myc Matrix [Re: agar]
    #4211714 - 05/23/05 10:10 PM (15 years, 5 months ago)

you are right. can't tell my ass from a hole in the ground when it comes to shrooms. just getting started with h poo, went and collected a pile a couple days ago and getting ready to pasturize. just a noob. can only hope to achieve theAGAR greatness. try to read everything you write man in hopes to have great grows, maybe someday when i have your experience level.


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Invisiblebackupwards
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Re: Far into the LC Myc Matrix [Re: backupwards]
    #4211725 - 05/23/05 10:14 PM (15 years, 5 months ago)

understood. better to roll with the odds and end up somewhere close to where you want to be than end up on another planet.
peace

can you hook me up to a good thread to clone shrooms?


Edited by backupwards (05/23/05 10:18 PM)


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Invisibleyousuck
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Re: Far into the LC Myc Matrix [Re: backupwards]
    #4211975 - 05/23/05 11:05 PM (15 years, 4 months ago)

is that mushroom pic 12.4g's or 124g's? if its the latter, that must be a pretty dense mushroom.


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Offlinemattymonkey
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Re: Far into the LC Myc Matrix [Re: agar]
    #4211982 - 05/23/05 11:07 PM (15 years, 4 months ago)

hey agar.. you would only clone a mushroom if you started from spores, right?


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Invisibleagar
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Re: Far into the LC Myc Matrix [Re: yousuck]
    #4212585 - 05/24/05 01:19 AM (15 years, 4 months ago)

Quote:

yousuck said:
is that mushroom pic 12.4g's or 124g's? if its the latter, that must be a pretty dense mushroom.




124G.....It was dense. Almost solid woody like stem.


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Invisibleagar
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Re: Far into the LC Myc Matrix [Re: mattymonkey]
    #4212599 - 05/24/05 01:25 AM (15 years, 4 months ago)

Quote:

mattymonkey said:
hey agar.. you would only clone a mushroom if you started from spores, right?




Where else is there to start? Unless you have perti's or LC's going.

Pick any crops grandmaster before/as the veil breaks. Use an internal tissue isolate from it - into an LC. If the LC goes well, you will get the genetics of that grandmaster, instead of the whole crowd. At least that is my train of thought.


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Offlinescarymidgets
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Re: Far into the LC Myc Matrix [Re: agar]
    #4212717 - 05/24/05 02:01 AM (15 years, 4 months ago)

did you just shoot the LC in the Vacutainer?

or did you mix it with some sterile water?

just curious


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Invisibleohmatic
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Re: Far into the LC Myc Matrix [Re: agar]
    #4212735 - 05/24/05 02:05 AM (15 years, 4 months ago)

Quote:

agar said:

LC STILL MATRIX  :tongue2:




THIS is so sexy ... omg  :stonedjerk:

makes me drool !
peace ohm :mushroom2:


--------------------
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InvisibleSoopaX
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Re: Far into the LC Myc Matrix [Re: agar]
    #4212743 - 05/24/05 02:07 AM (15 years, 4 months ago)

that stirbar idea is awesome :smile: i'll be trying it soon


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Invisibleohmatic
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Re: Far into the LC Myc Matrix [Re: SoopaX]
    #4212750 - 05/24/05 02:10 AM (15 years, 4 months ago)

Quote:

SoopaX said:
that stirbar idea is awesome :smile: i'll be trying it soon




BUYING you mean :wink:
as the stirrbar isnt worth shit without the magnetic stirr plate.
peace ohm :mushroom2:


--------------------
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InvisibleLiquidkick
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Re: Far into the LC Myc Matrix [Re: ohmatic]
    #4212759 - 05/24/05 02:14 AM (15 years, 4 months ago)

I forgot to add a marble or whatever to my LC, is this gonna make it difficult to break it up?


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InvisibleRoadkillM
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Re: Far into the LC Myc Matrix [Re: Liquidkick]
    #4212938 - 05/24/05 03:38 AM (15 years, 4 months ago)

Quote:

Liquidkick said:

I forgot to add a marble or whatever to my LC, is this gonna make it difficult to break it up?




yeah it will


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Re: Far into the LC Myc Matrix [Re: Roadkill]
    #4213029 - 05/24/05 04:16 AM (15 years, 4 months ago)

Best shake it up everyday while it is still weak enough to break up, after a few days on its own it forms a tight ball which is hard to suck up, like a ball of cotton wool in water. You can also add so shards of broken glass


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Invisibleagar
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Re: Far into the LC Myc Matrix [Re: scarymidgets]
    #4213129 - 05/24/05 05:13 AM (15 years, 4 months ago)

Quote:

scarymidgets said: did you just shoot the  LC in the Vacutainer? or did you mix it with some sterile water? just  curious




Either way works. If you want to store it for years? Simply set a test tube with a septa plug top (or vacutainer)upright overnight.

The myc will all settle to the bottom.

Gently insert a syringe needle & asperate out 95% of the LC fluid.
Then, inject sterile water.
Then place tube in the fridge.
It will remain viable for years.


Like a swirling nebula :stoned:


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OfflineInterested
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Re: Far into the LC Myc Matrix [Re: agar]
    #4214794 - 05/24/05 05:30 PM (15 years, 4 months ago)

People have proposed that the limiting factor in LC growth is oxygen supply. It'd be great to see some confirmation of this. Have you ever tried bubbling (HEPA filtered) air through your LC cultures? (Realize this is a touch off topic, but it didn't seem worth starting a new thread over)


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Invisibleagar
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Re: Far into the LC Myc Matrix [Re: Interested]
    #4215074 - 05/24/05 06:31 PM (15 years, 4 months ago)

Quote:

Interested said:
People have proposed that the limiting factor in LC growth is oxygen supply. It'd be great to see some confirmation of this. Have you ever tried bubbling (HEPA filtered) air through your LC cultures? (Realize this is a touch off topic, but it didn't seem worth starting a new thread over)





Tubing filter lids & the vortex action of a magnetic stir plate bar cause/create sufficient gas exchange to overcome that limiting factor.

So much so that, if left at room temperatures & not agitated/stirred on a daily basis, mycelia will grow a thick mat on the liquids surface. Then, if exposed to adequate light, the surface of the mat will also begin to form primordial knots & pins :grin:. Which will most often cause the floating Mat to tip over :eek:, as it becomes top heavy. :rolleyes:

Best to refrigerate a healthy culture around 40F in darkness, once it is thick with mycelia, in order to stall the growth process.


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OfflineInterested
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Re: Far into the LC Myc Matrix [Re: agar]
    #4215615 - 05/24/05 08:15 PM (15 years, 4 months ago)

That's the most impressive LC picture I've seen in my life!

So the "tubing filter lid" is nothing more than a lid into which a tube filled with polyfil has been sealed, right? Presumably a financially constrained person could use a drinking straw and some hot melt glue?


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Invisibleagar
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Re: Far into the LC Myc Matrix [Re: Interested]
    #4215730 - 05/24/05 08:36 PM (15 years, 4 months ago)

Quote:

Interested said:
That's the most impressive LC picture I've seen in my life!

So the "tubing filter lid" is nothing more than a lid into which a tube filled with polyfil has been sealed, right? Presumably a financially constrained person could use a drinking straw and some hot melt glue?




Not sealed. Polyfil is lightly stuffed into the tubing, to allow gas exchange. Rubber vacuum tubing (auto parts store, True Value or Ace Hardware) is less than a buck a foot & a tube of silicone under $4.

Glue gun glue & a drinking straw will not work, a PC temps would melt them.


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Re: Far into the LC Myc Matrix [Re: agar]
    #4215799 - 05/24/05 08:52 PM (15 years, 4 months ago)

should vaccutainers be completly filled with lc or small amount of liquid culture and sterile water?


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Invisibleagar
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Re: Far into the LC Myc Matrix [Re: 5meopsyco]
    #4215814 - 05/24/05 08:56 PM (15 years, 4 months ago)

Quote:

5meopsyco said:
should vaccutainers be completly filled with lc or small amount of liquid culture and sterile water?




For very long term storage, a vacutainer should contain mycilia & sterile water. Shorter term, just straight LC is fine - so long as it is kept refridgerated.


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OfflineHippieChick
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Re: Far into the LC Myc Matrix [Re: Interested]
    #4215861 - 05/24/05 09:08 PM (15 years, 4 months ago)

Yes, Agar was nice enough to hook me up with this idea not too long ago. For a few dollars and about an hour, I had eight of these ready to go.



They work great. Just make sure your plastic lid is a 1/4 turn lose while PC'ing and tighten before removing from PC. Thanks Agar. You're such a sweetheart.
Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


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Offline5meopsyco
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Re: Far into the LC Myc Matrix [Re: HippieChick]
    #4216356 - 05/24/05 11:23 PM (15 years, 4 months ago)

about how much lc to water ratio?


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Re: Far into the LC Myc Matrix [Re: 5meopsyco]
    #4217271 - 05/25/05 04:24 AM (15 years, 4 months ago)

Aerated LC is important, during boiling most of the gases are driven out of the LC. You should shake it very well to aerate the mixture. I have described this as the catch 22 of filter patches, since if you shake a jar with a filter it will soak the filter allowing contams to grow from the outside through the filter, so with no filter you can shake jars very well every few days which both aerates and breaks up mycelium.
Agars ingenious jars have rubber tubes which can be pinched while shaking so the filter is not going to get wet.
Agar- maybe you could make a little raft for the mycelium mat to grow into so it would not topple over.


Edited by blackout (05/25/05 04:25 AM)


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OfflineInterested
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Re: Far into the LC Myc Matrix [Re: blackout]
    #4220036 - 05/25/05 10:02 PM (15 years, 4 months ago)

Quote:

maybe you could make a little raft for the mycelium mat to grow into so it would not topple over.




I've been planning to try just this with a wide mesh screen of some sort. I'll report back if I have any success.


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Invisibleagar
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Re: Far into the LC Myc Matrix [Re: Interested]
    #4220162 - 05/25/05 10:53 PM (15 years, 4 months ago)

It would be interesting to cut an inch thick open cell sponge to fit snugly inside the liquids surface in a quart jar of LC, as a platform for primordia to form on.

The result could be hydroponic shrooms :eek: :mushroom2: :stoned:


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InvisibleDexter_Morgan
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Re: Far into the LC Myc Matrix [Re: agar]
    #4220345 - 05/25/05 11:36 PM (15 years, 4 months ago)

Someone has to try! Then with Poo water!


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Re: Far into the LC Myc Matrix [Re: Dexter_Morgan]
    #4220431 - 05/25/05 11:52 PM (15 years, 4 months ago)

Quote:

For very long term storage, a vacutainer should contain mycilia & sterile water.




How does one get the mycelium out of the LC to put it in the sterile water?


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Re: Far into the LC Myc Matrix [Re: Blue Helix]
    #4220521 - 05/26/05 12:13 AM (15 years, 4 months ago)

Quote:

Blue Helix said:
Quote:

For very long term storage, a vacutainer should contain mycilia & sterile water.




How does one get the mycelium out of the LC to put it in the sterile water?






I think I explained in this thread somewhere.

Asperate culture out of jar, inject into vacutainer. Stand near full vacutainer upright (in a cup or whatever) & set it in the fridge overnight.

By the next day all the myc in it should have settled to the bottom of it.

Insert sterile syringe (gently) & asperate out 95% or more of the LC solution, leaving the myc. With another syringe, inject sterile water into the vacutainer.


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Re: Far into the LC Myc Matrix [Re: agar]
    #4220568 - 05/26/05 12:21 AM (15 years, 4 months ago)

AWESOME! Thanks. I love LCs!


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OfflineInterested
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Re: Far into the LC Myc Matrix [Re: agar]
    #4220591 - 05/26/05 12:26 AM (15 years, 4 months ago)

Quote:

It would be interesting to cut an inch thick open cell sponge to fit snugly inside the liquids surface in a quart jar of LC, as a platform for primordia to form on.




I've already tried this, in fact! Alas, mycelium growth wasn't near enough to begin pinning, or even enough to really determine whether it had penetrated the sponge. Hopefully your air exchange technique will prove the missing piece of the puzzle.


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Re: Far into the LC Myc Matrix [Re: Interested]
    #4220741 - 05/26/05 12:56 AM (15 years, 4 months ago)

Quote:

Interested said:
Quote:

It would be interesting to cut an inch thick open cell sponge to fit snugly inside the liquids surface in a quart jar of LC, as a platform for primordia to form on.




I've already tried this, in fact! Alas, mycelium growth wasn't near enough to begin pinning, or even enough to really determine whether it had penetrated the sponge. Hopefully your air exchange technique will prove the missing piece of the puzzle.




I think you will need a magnetic stir plate for optimal tests.


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OfflineInterested
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Re: Far into the LC Myc Matrix [Re: agar]
    #4220967 - 05/26/05 02:16 AM (15 years, 4 months ago)

Quote:

I think you will need a magnetic stir plate for optimal tests.




Yep, I have one. Were you stirring constantly, or once or twice a day? Can you make a rough estimate of the RPM you were using? Obviously too high, particularly toward the end, would cause mycelium fragmentation detrimental to fruiting (although obviously beneficial for someone looking to make an LC syringe).

Also, thanks for your help. I hadn't expected the luxury of having this much prior work done for me. It's reassuring to think I won't be starting from scratch.


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OfflineAeolus1369
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Last seen: 11 years, 8 months
Re: Far into the LC Myc Matrix [Re: agar]
    #4222741 - 05/26/05 03:36 PM (15 years, 4 months ago)

Awesome work as always!

What's your LC recipe by the by?


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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