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OfflineTrip_Messiah
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Tyvek on a LC.
    #4392550 - 07/11/05 03:25 PM (11 years, 4 months ago)

Hello. I am going to be starting a liquid culture soon and was planning on using tyvek as a lid and still use the metal band to hold it on. Now my concern is what would happen if some of the water splashed on the tyvek when I shake the jar would it do any harm? If so then what would be a good alternative? Thanks!


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Invisiblemetasin
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Re: Tyvek on a LC. [Re: Trip_Messiah]
    #4392602 - 07/11/05 03:42 PM (11 years, 4 months ago)

I don't think that would be a great idea. Try filling a jar up with water and do the tyvek thing and shake it. If you turn it upside down, (at least with USPS tyvek), a few little drops of water will come out. Since the karo/honey/dextrose or whatever you're using and water is miscible... you see where I'm going here?


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Offlineskaos
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Re: Tyvek on a LC. [Re: metasin]
    #4392643 - 07/11/05 03:52 PM (11 years, 4 months ago)

A wet tyvek is an invitation for contams. Either avoid the tyvek from being wet or use someting else. I personally use baby bottle without any kind of filtration, the hole in the nipple are enought to provide air to the LC. With the little platic cap on the baby bottle are a perfect container for LC. They can be PC'ed too or microwaved both work fine. See agar BB Tek for more info.

skaos


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OfflineTrip_Messiah
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Re: Tyvek on a LC. [Re: metasin]
    #4392693 - 07/11/05 04:08 PM (11 years, 4 months ago)

I read that the baby bottle tek doesn't get enough air exchange. That's why I was wanting to try something else. I've seen a few people do LCs with jars. I just wanted to find a way to do it without drilling holes in the lids.


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Edited by russtafari (07/11/05 04:09 PM)


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Offlineunknown22
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Re: Tyvek on a LC. [Re: Trip_Messiah]
    #4393146 - 07/11/05 06:20 PM (11 years, 4 months ago)

Just take the jars mix up all your liquids,put in marble cover with plastic wrap and put in microwave for 5 minutes. Take out place lid with hole in it on.


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Offlineskaos
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Re: Tyvek on a LC. [Re: Trip_Messiah]
    #4395141 - 07/12/05 08:52 AM (11 years, 4 months ago)

They get enough air, LC dosent need much fresh air. I'll show u pics when i gets home toonight, BB is the shit, u can swirl do anyting you want without any doing any mess.


skaos


Edited by skaos (07/12/05 08:53 AM)


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InvisibleFooManM
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Re: Tyvek on a LC. [Re: Trip_Messiah]
    #4395226 - 07/12/05 10:27 AM (11 years, 4 months ago)

Quote:

russtafari said:
I read that the baby bottle tek doesn't get enough air exchange. That's why I was wanting to try something else. I've seen a few people do LCs with jars. I just wanted to find a way to do it without drilling holes in the lids.




It would be really hard to use the Tyvek without drilling the lids. I use quart jars with Tyvek under the lid. I drill 2 holes in the lid, one is about 1/2" and the other is just big enough for inoculating with a spore syringe. I put polyfill in the bigger of the two holes and masking tape over the smaller one. I cover the whole thing with foil and PC. This allows good gas exchange and the only exposure the Tyvek gets to potential contaminants is during inoculation, when the small hole is exposed. This works 4 me, but I'm sure there are better ways. :thumbup:


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OfflineIGnosticAbhorI
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Re: Tyvek on a LC. [Re: skaos]
    #4395229 - 07/12/05 10:28 AM (11 years, 4 months ago)

Quote:

the hole in the nipple are enought to provide air to the LC.




Wrong.

Quote:

LC dosent need much fresh air




Kind of wrong.

Nipple should have silicone in it, to make it a Self-Healing injection site.

To much fresh o2 is bad....But sufficating it with co2 isn't great either...

Just some FYI

-Gnostic


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Offlineskaos
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Re: Tyvek on a LC. [Re: IGnosticAbhorI]
    #4395979 - 07/12/05 03:45 PM (11 years, 4 months ago)

I may be wrong, im no god but all i know is that it works very well for me in fact better than any other LC tek i tried. No need to seal hole in nipple with sillicone. No myc stalling, no contams, no troubles at all.

But of course IGnosticAbhorI is more experienced than i am so forget what i said :smile:

ill post some pics of my LC when i get back home

later,


skaos


Edited by skaos (07/12/05 03:46 PM)


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OfflineJellyRollMorton
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Re: Tyvek on a LC. [Re: Trip_Messiah]
    #4396777 - 07/12/05 07:09 PM (11 years, 4 months ago)

russta if you don't want to drill just use a hammer and something pointy and that works great for me. you just have to remember to flatten out the metal from the other side as it will poke through tin foil and scratch tyvek if left as is. Im not sure if it will puncture tyvek as i remember to flatten it now.


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