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OfflineMych
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Registered: 03/25/05
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Last seen: 12 years, 1 month
Mych in LC going from white to brownish? wtf
    #4268744 - 06/07/05 06:28 PM (12 years, 11 months ago)

why would the mycelium in my liquid cultures (5% karo) start growing a little bit and then stop.. and turn brownish? Ive been having issues getting a single LC to work for me... no visible contams, but it simply isnt working for me.


this is how it was for a while.. i swirled the water around and you can see the small little bits of mych in there.. it hasnt grown much since, and then it turned a brownish color (not the water.. the white substance itself turned brown)


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Offlinemockeylock
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Re: Mych in LC going from white to brownish? wtf [Re: Mych]
    #4268752 - 06/07/05 06:31 PM (12 years, 11 months ago)

I'd ask in the contam forum.....cause that's probably what you're looking at.


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OfflineMych
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Registered: 03/25/05
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Re: Mych in LC going from white to brownish? wtf [Re: mockeylock]
    #4268771 - 06/07/05 06:39 PM (12 years, 11 months ago)

Quote:

mockeylock said:
I'd ask in the contam forum.....cause that's probably what you're looking at.



will do. would contams actually cause legit mych to turn brown? ive actually put a piece of known mych into a LC syringe and its turning brown after only a few days. same happened with a multispore LC


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Offline_blitzer
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Registered: 03/28/05
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Re: Mych in LC going from white to brownish? wtf [Re: Mych]
    #4268791 - 06/07/05 06:47 PM (12 years, 11 months ago)

Quote:

Mych said:
why would the mycelium in my liquid cultures (5% karo) start growing a little bit and then stop.. and turn brownish? Ive been having issues getting a single LC to work for me... no visible contams, but it simply isnt working for me.


this is how it was for a while.. i swirled the water around and you can see the small little bits of mych in there.. it hasnt grown much since, and then it turned a brownish color (not the water.. the white substance itself turned brown)






I had similar trouble w/ LC a year ago....

This summer i used 2% Karo Light w/ 2% light(est available) malt extract and it worked fine.

Multispore both times, but different source so I cannot attribute it to a change in medium.


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OfflineMych
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Registered: 03/25/05
Posts: 415
Last seen: 12 years, 1 month
Re: Mych in LC going from white to brownish? wtf [Re: _blitzer]
    #4269014 - 06/07/05 07:57 PM (12 years, 11 months ago)

Quote:

_blitzer said:
Quote:

Mych said:
why would the mycelium in my liquid cultures (5% karo) start growing a little bit and then stop.. and turn brownish? Ive been having issues getting a single LC to work for me... no visible contams, but it simply isnt working for me.


this is how it was for a while.. i swirled the water around and you can see the small little bits of mych in there.. it hasnt grown much since, and then it turned a brownish color (not the water.. the white substance itself turned brown)






I had similar trouble w/ LC a year ago....

This summer i used 2% Karo Light w/ 2% light(est available) malt extract and it worked fine.

Multispore both times, but different source so I cannot attribute it to a change in medium.




good to know. perhaps i can try lower sugar concentrations.


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