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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Stro's Cleaning and Isolating On Agar [Re: Rotnpins]
    #27794266 -

Stro:

Great stuff.

I link your clone tek in my write up for noobs:
Things I wish I'd known when I started here.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27916254 -

Very useful post. Well written and pictured.


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27916328 -

Very informative write up!! Thanks so much. I've got a batch of transfers I need to do in the next couple days, so this couldn't have been more timely. It will help me make much better informed decisions on transfer site selection.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27923585 -

This is amazing. Just started my first agar plates & assume I'll need this info in a few days. This forum is such a gold mine of information. Thanks Stro!


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Why Syringe to Grain will end badly
Semi-Solid LC
EZ Dial Mono Tub
CryoVial Long Term Storage
The Ol' Chocolate Bar

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27933211 -

I just read this 9 yrs and 2 mo later and have improved my technique ten-fold just by reading. Adding this to my threads because it's still helping someone to this day.


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"I don't care what congress does, so long as they don't do it in the streets and startle the horses." -Victor Hugo

>>>LOVE YOURSELF........<3----------:mushroom2::heart::thumbup::thumbup::heart::mushroom2:--------;)........HELP OTHERS>>>

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27933228 -

Glad this got bumped up so I can see it. This is good information. Thanks


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Namo Tassa Bhagavato Arahato Samma Sambuddhasa
:kratomtee:

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Re: Stro's Cleaning and Isolating On Agar [Re: Zigzagzin] * 1
    #28181595 -

Bumping yet again for the sme reason :-)


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Stro's Cleaning and Isolating On Agar [Re: Janus62]
    #28219528 -

Very helpful article!

I've been working with Agar and accidentally left a flask with Agar open to the air in the room I do my work. I noticed quite a few growths that were white and would be difficult to distinguish from exotics mycelium, and I'm wondering

How does one ensure the culture isn't mixed with other white mycelium and if it is, how does one separate them?

I'm thinking maybe I need to make a special dish or maybe transfer to a slide and view under a microscope to look for spores (indicating lower fungi)...?

Then, if I can't find a clean sector, how can you separate them? I've heard pour Agar over it and only the higher fungus will survive/grow through the Agar, but I tried this with P. cinct mycelium and it seems to have died.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #28414516 -

Wow this came right when I was about jump into agar semi blind. I feel so much more confident now about this, thanks to this post and bod's sab tek. Thank you!

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #28569198 -

Glad I found this detailed step by step of agar to isolate from contamination. Learning how to approach selecting for monoculture is going to be a huge help for me! Thank you Stro

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Re: Stro's Cleaning and Isolating On Agar [Re: Buddy20916]
    #28601297 -

I'm going to use this information, even so many years later it seems valid


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just a man looking for new adventures

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Re: Stro's Cleaning and Isolating On Agar [Re: Sikjakass]
    #28601371 -

It is very valid! Still a go to link I use to teach agar!


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #28651168 -

Thank you very, very much.  Your pics brought a few things straight on home for me.  I am grateful for the information.


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gr8fl.studio

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #28722037 -

🫡

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #29099283 -

Great post but there are some very wrong parts in the middle (the part on "sectors" and "monocultures") that time has proved wrong.

I tried to contact Stro to see if he'd correct it, but he didn't reply and hasn't logged in for 7 years (since 2018).

So I copied his post and re-posted an edited version, with credit, here:
https://www.shroomery.org/forums/showflat.php/Number/29095187

People should use that and several TCs agree.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Stro's Cleaning and Isolating On Agar [Re: nektar61] * 2
    #29099358 -

Link me to this thread proving your theory


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Stro's Cleaning and Isolating On Agar [Re: cronicr] * 1
    #29100639 -

:whathesaid: it would be helpful if you highlighted the "incorrect" parts

I don't have time to compare the two, but im guessing something about anastomosis causing sectoring to be an unlikely or even impossible rout to a monoculture?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Stro's Cleaning and Isolating On Agar [Re: tryptkaloids]
    #29100752 -

sectoring could be caused by a number of things that u wouldnt be able to tell by visuals alone

i do agree with the notion that trying to isolate a "monoculture" thru just transferring sectors is probably not the way to go about it

but i dont think saying that bc something sectors that it 100% isnt a true isolated monoculture, the fact is u wouldnt know anyway

theres gourmet mushroom papers about commercial production strains that seem to suggest degradation of the culture was the cause of sectoring or that it was thought to be the case


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∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Stro's Cleaning and Isolating On Agar [Re: cronicr]
    #29101475 -

Mutation also happens spontaneously, and could cause sectoring


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Shop: North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags

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