Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Sporeworks.EU Spores for European Microscopy   MagicBag.co Certified Organic All-In-One Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Mono Tub Substrate

Jump to first unread post Pages: 1 | 2 |  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
time to get to the bottom of psilocybin
    #13191380 -

Alrite, time to get to the bottom of when and why some mushies have large amounts of Psilocin and psilocybin. First, don't post if it is going to piss me off, you won't like me when I am mad. Second, I am not some teen junkie. Third, just like the marijuana plant during the final stages of bud development thc is used as a "defence." turning the lights off for 4 hours in the middle of the day confuses the marijuana plant and it starts to produce thc, useful trick for your personal sack right? Well what tricks make mushies pump out the good alkaloids? Dunno, never even researched it, but I intend to find out.

I will be doing a myriad of controls and extractions on numerous cultures, stay tuned.

The second poll question is asking tor opinion. Is there a correlation between techniques and mushies strength, yes means no correlation.. no means there is a correlation.
worth the effort?
You may choose many
there is no correlation in alkloid development
You may choose only one


Votes accepted from 09/13/10 10:18 PM until the end of time
You must vote before you can view the results of this poll


Edited by jaye (09/13/10 10:55 PM)

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: jaye]
    #13191393 -

Definitely worth it if you have the means of testing such a thing.


I dont understand your second question.

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: mukhail]
    #13191409 -

Do it up.
I voted yes to your second question, even though I have no idea what you were asking.
As a helper note, i'm pretty sure most of the psilocybin production takes place when theyre pins or small, people are always saying that the pins get you just as high as the caps. Also potency is influenced by nutrients available in the substrates (people say shrooms grown on coffee are more potent)


--------------------
Whoa; I can see the music.

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: jaye]
    #13191420 -

Substrate makes a very very small negligible difference if any. The contributing factor to potency is the strain that you isolate (or that appears in a multispore grow)

The more nutrients in the soil will give you more flushes, and bigger yields, but potency will not be affected.


--------------------
Psychedelics are the only capable thing of pushing one's mind to the edge of infinity....

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: Doctopus]
    #13191424 -

Doctopus said:
Substrate makes a very very small negligible difference if any. The contributing factor to potency is the strain that you isolate (or that appears in a multispore grow)

The more nutrients in the soil will give you more flushes, and bigger yields, but potency will not be affected.


:yesnod: :thumbup:


--------------------
7 days without waves makes one weak!

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: Mad_Hatter2004]
    #13191453 -

I dunno i just read that in another post here, Ive never used coffee because it contams so easily, and I said influenced, not enhanced, or anything that substantial


--------------------
Whoa; I can see the music.

Edited by ChordProgression (09/13/10 10:36 PM)

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: jaye]
    #13191454 -

How do you plan to measure potency?


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: Doc_T]
    #13191488 -

Best way to measure potency in my opinion....

Well we all know Psilocybin/Psilocin are alkaloids! So get out that ethyl (or methyl haha i get them confused) alcohol and lets do some dissolving.

Grind your shrooms into powder and get a funnel and filter paper. Put the filter paper in the funnel and then set the mushroom matter on the funnel.

Now begin by adding alcohol to "rinse the mushrooms" Collect the alcohol in the flask, but dont go overboard with the rinsing because you will have to evaporate off the alcohol.

You will want to then let the filter paper with mushrooms on it soak in a new set of alcohol. Let this sit for about a day, and then collect the alcohol.

You will keep collecting the alcohol in every step and saving it because this contains the precious alkaloids. Let the mushrooms sit in the alcohol for about 12-24 hours and then filter off the excess matter. Do this a few times......

Now boil all the alcohol off at about 180 degrees Fahrenheit and you will be left with a powder. This powder if strained enough in the previous steps should be nearly all alkaloids made up of the active chemicals in "shrooms."

Weight the powder and wallah! You now know how much said weight mushroom produces in psilocybin/psilocin and Baeocytsin or however you spell it.


--------------------
Psychedelics are the only capable thing of pushing one's mind to the edge of infinity....

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: Doc_T]
    #13191517 -

Gas chrom, sporry about the last poll question, I worded it poorly., I will.modify my original post to make it more clear. I am on my phone atm.

I will extract with ethanol under the correct conditions. Yup yup about the substrate, that will be a control. Along with atmospheric conditions and environment.

If the small pins have just as much psilocin then based on my knowledge I would say psilocin is a metabolic pathway to a useful molecule to the cell structure. If the psilocin is present on the pin or the pins small base, that would provide me with more information to speculate with.

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: Doctopus]
    #13191522 -

I would try to avoid boiling he alcohol, or changing the pressure. This may hurt various alkies

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: jaye]
    #13191541 -

You guys read the Potency Project thread yet? You might want to.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: Doc_T]
    #13191561 -

No, I will try to if I get the time.

If I got enough information, I may be able to find out how the mushies uses the psilocin, but I'm a long way off.  it is safe to say though, that the mushroom cells have receptors, do their cells use psilocin as a neurotranmitter? Hmm. I am thinking this over too much, I need to return to basics

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: jaye]
    #13191596 -

My burning question is if the cells use psilocin for the modulation of elements and compounds in and out of the cell. Is more psilocin bioavalible at certain times or under certain conditions?

For example if the cell needs environmental compound "a" to enter the cap for a myriad of options/reasons and alkloid "a" allows environmental compound "a" to enter the cell, you can prevent alkloid "a" from doing its job and more alkloid "a" will become bioavalible.

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: jaye]
    #13191610 -

How would you measure that?


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: Doc_T]
    #13191707 -

I don't know.if I will be able to accurately... I will try gas chrom during various stages of growth. This will show me the types of alkloids present during stages. Also, I will be able to stain the cells of the mushie and find out exactly where the alkloids are reacting on the cell..... most of this is for my own speculation, and I will provide pictures and copies of my lab books for you all to make your own speculations, and try to copy my recipes for potent mushies.

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: jaye]
    #13191898 -

Hmmm this all sounds fine and dandy, but I hope you either A) have access to a college lab or B) Have lots of money.

If "A" applies then I hope you also have A)big enough balls to bring active containing mycelium or fruiting bodies to your school, or B) a small enough brain that the danger or this doesnt register.


--------------------
Psychedelics are the only capable thing of pushing one's mind to the edge of infinity....

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: Doctopus]
    #13191909 -

It always comes down to the same thing- somebody needs to get a GCMS. :sadyes:


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: jaye]
    #13191923 -

Oh well, the 2nd question is really worded backwards and you won't get much of a useful response with it set up that way.  I voted, Yes, there is a correlation... :shrug::laugh2::dancingbear:

Peace
-PS


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: PrimalSoup]
    #13191999 -

PrimalSoup said:
Oh well, the 2nd question is really worded backwards and you won't get much of a useful response with it set up that way.  I voted, Yes, there is a correlation... :shrug::laugh2::dancingbear:

Peace
-PS


PrimalSoup said:
Oh well, the 2nd question is really worded backwards and you won't get much of a useful response with it set up that way.  I voted, Yes, there is a correlation... :shrug::laugh2::dancingbear:

Peace
-PS


Sorry about that question/poll. Yep, I sure can get my hands on a spec machine! I am a forum newbie, I am not sure why I even added a poll ..  logic implies that there has to be a correlation between external factors and mushroom alkloid content let alone mushroom growth stages themselves. I am no myologist ...I think that's study of mushroom cultures.... but I am a chemist with medical research background, and I am fairly sure within the next year I will have a great conclusion and process completed too. Hopefully get some recognition for the mushroom alkloids. There is much benefit to be found in the countless alkloids found in all mushrooms for the scientific community.... why are these lil buggers illegal again?

Extras: Filter Print Post Top
Re: time to get to the bottom of psilocybin [Re: jaye]
    #13192077 -

Well I am off for the night, this thread is not going to be my "culture" thread, but I will check this thread and try to respond. if anyone has questions my degree might have answers. Neuroscience is more or less my speculation specialty heh.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 |  [ show all ]

Shop: Sporeworks.EU Spores for European Microscopy   MagicBag.co Certified Organic All-In-One Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Mono Tub Substrate

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* pinning only on bottom of cake arex7tt 1,043 7 09/04/03 07:02 PM
by arex7tt
* looking for something to protect the bottoms of my trays (rack set up) moshe1 238 9 05/07/21 03:57 AM
by moshe1
* Mycelium contains psilocybin, right? Fd3000 15,594 6 06/06/02 02:21 PM
by psyconaut
* Test for psilocybin in mushroom? Riboflavin 697 1 03/06/05 12:41 PM
by PsiloPsyKo
* Re: psilocybin/psilocin extraction tek
( 1 2 all )		 Psychonaut 10,100 36 06/28/00 05:17 PM
by Anonymous
* Is it possible to grow a cubensis that has NO psilocybin?
( 1 2 all )		 Baby_Hitler 5,770 33 12/17/03 05:37 PM
by Rose
* Do aborts have more psilocybin??
( 1 2 3 4 all )		 resin 20,530 71 04/26/03 05:06 PM
by ExtravagantDream
* Psilocybin absorbtion through skin?
( 1 2 all )		 angryshroom 6,893 24 08/20/13 10:40 PM
by Synocybin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,453 topic views. 19 members, 325 guests and 243 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.059 seconds spending 0.026 seconds on 22 queries.