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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: Glowing Shrooms-- Tek?
    #91056 -

If they can do it with nude mice they can surely do it with mushrooms.

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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91058 -

that was my first thought too as soon as I had seen the glowing fungi, but I don't think anyone short of DNA research specialist that loves to shroom that could accomlish this task... : (. Anyone here have a DNA-splitting lab, now I wish I had enrolled in some Bio-technology field here at the U-W instead of engineering, and I'd be in the lab now trying to split em' instead of talking about em'.

-peace


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91059 -

Which mushroom species produce bioluminescence?

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Why is it that we kill people who kill people to show people that killing people is wrong?


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91060 -


More importantly than which species is responsible for the bioluminescence is WHAT actually makes them glow.
If anyone knows the chemical pathway (in detail) that produces the glow, it would help in proposing an experiment to get this project underway. The Glowing Psilocybe, that would be so nice.


"After the people are dead, after the things are broken and scattered, taste and smell alone, more fragile but more enduring, more unsubstanial, more persistent, more faithful, remain poised a long time, like souls, remembering, waiting, hoping, amid the ruins of all the rest."
-Marcel Proust


[This message has been edited by Sclorch (edited December 22, 1999).]



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Note: In desperate need of a cure...

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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91061 -

I had originally talked about this in my first post in Genetically Mutated Mushrooms but I've never persued it until now. I've found a few sources...here:

Miska-Publikationen
http://www.med.uni-giessen.de/derma/Miska-PublikationenE.htm

Determination of sterilization effectiveness by measuring bacterial growth in a biological indicator through firefly luciferase determination of ATP.
http://opbs.okstate.edu/~leach/Bioch5853/Administr ative/%20%20Instructor/reference%20links%20folder/JBB%202%20129-133%2088

APBiotech - Biotechnology Products such as Luciferin!
http://www.apbiotech.com/

Glow-In-The-Dark Genes - Prolume Ltd.
http://www.prolume.com/

When I got to this last site I realized I found exactly what I was looking for. This site is so cool. I especially liked the www.biobay.com site. I would love to go for a midnight swim in that bay under the influence of some good shrooms. I started getting ideas for creating a swimming pool and/or a floatation tank with those non-toxic dinoflagellates.

If anyone is interested in finding out more information, I'll do some more researching.

-PsiliPharm

[This message has been edited by PsiliPharm (edited December 22, 1999).]


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91062 -

This would be a very long process. Also, when the spores of the artificially grown, bioluminecent mushroom are inoculated into a substrate, the strand of DNA that was need to make the mushroom glow would not be passed down to other mushrooms.

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My mind is corrosive...


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91063 -

One way I think this is possible is the addition of the gene that is responsible for the Luciferase enzyme and insert Luciferin into the substrate. Or wait until after the shrooms have been harvested and then depending on the method of ingestion, mix it with the luciferin in water? But then again, the idea is to make a self-sustaining bioluminescent psychedelic mushroom. Still working on that one. One thing that is interesting to me is the pre-fix to the chemicals mentioned above, Lucifer. Strange, I would like to know who came up with that name and why.

-PsiliPharm


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91064 -

I just realized that Luciferin is an indole just like Psilocin/Psilocybin and DMT and all the other Tryptamines. Interesting... This may mean that it can be altered by the enzymes already present in the shrooms, mainly the 4-hyroxylation enzyme and then the 4-phosphorylation enzyme. I doubt that it will change anything significantly.

-PsiliPharm


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91065 -

Please continue in this topic... Its amazing!!!!
Nacho

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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91066 -


Mother Fucker!!!!!! I just erased my entire post! Aaaaaaahhhhhhhhhhhhhh!

Hayabusa- If you knew how basic DNA replication worked, you would know that the insert DNA would be replicated with the original DNA. And we wouldn't be working with spores, only individual cells. After we get the Dikaryotic tissue (w/ the insert DNA) to grow, the process of gametogenesis would produce spores that have the inserted gene.

A few questions needed to be answered:
oh yeah, let's assume we're using Psilocybe cubensis.

1. How many chromosomes does a monokaryotic cell contain?

2. Which chromosome would be the best to contain the insert gene?

It looks like luciferase would be an ideal candidate since it is an indole, but would that affect psiloc(yb)in content of the shroom?
If so, then that is not what we want. Ideally, we want a compound that:

a) produces bioluminescence
b) does not decrease psiloc(yb)in content
c) does not need any substrate additives, so that it can grow in the wild.

What is great about this process is that it would only have to be performed ONCE!!!
After that, it's just a matter of bulk growing and distribution of the spores!



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Note: In desperate need of a cure...

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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91067 -

Hi people I've got an idea.
I know of one mutagen, that causes change in DNA (perminant changes, for generations to come), in accordance to its surroundings.
For example, there is a deseas that kills lots of lab rats. With this mutagen, cells resistable to the virus were created, by puting a cell in a viral enviroment.
This mutagen is called EMS (ethyl methane sulfonate (sp?)). I was thinking of making 10-20 culturs on petridishes (agar), with the addition of EMS and luciferine. This will cause the shroom to mutate and produce a new strain, hopefull between the 20 cultures, one glowing strain, will also be created. This is working on luck, instead of pure science, but lets say that if 5 people would do this experiment, togather they will easily have 100 dishes, or more, increasing the chances of such mutation to occur.

Later,
tweedy.


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91068 -

Thanks tweedy, this is a very interesting substance. I'll have to do some more research on it. I wish you would have said something about it sooner. Especially, if you've read Genetically Mutated Mushrooms. Wouldn't it be better to use Luciferase? Or wouldn't it be possible to do it twice once for Luciferase and then again for Luciferin. I'm about to head to the library. The day after Christmas and I'm already back to work.

-PsiliPharm


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91069 -

tweedy, could you please post a source of your information, this is definitely a mutagen but I haven't found any specific information about it doing what you say it does. Here are a few other mutagens which are often compared to EMS:

methyl methanesulfonate
N-nitroso-N-ethylurea
N-methyl-N-nitrosourea

And I've listed many different anti-mitotic substances in Genetically Mutated Mushrooms.

-PsiliPharm


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91070 -

It was posted in a hebrew science magazine.
The research was done here in Israel, and even if I gave you the source, you wouldnt be able to read it. I can try and translate it for you, but it'll take sometime.

later,
tweedy.


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91071 -


I think some of you are missing the point.

This is not a MUTATION thread.
We're not making polyploid subspecies of hallucinogenic mushrooms.

I'm talking about actually inserting the gene that codes for the glow effect into a piece of shroom DNA that was taken from a shroom cell and then putting the entire piece of DNA back into the cell and getting it to grow.

I'm sure this will be a real bitch of a process. You may get only one cell out of 100 cells to actually grow. But as I have said before-- THIS PROCESS ONLY NEEDS TO BE PERFORMED ONCE!!

After you got these glowing shrooms growing, it's just a matter of bulk-growing.



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Note: In desperate need of a cure...

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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91072 -

just thought id repost an old topic

------------------
all in a dayze work



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" I'm somewhere where I don't know where I am. "

"Experience is the meaning to life" -me

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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91073 -

Lucifer, [lux=light, fer='fero' meaning to bear, 'bear light'. Satan used to be god's favorite or most powerful angel or something like that. The 'ase' is an enzyme ending, so luciferase is an enzyme that bears light. I could be wrong, but I thought luciferase was one of those 'firing' enzymes, that once fired [by releases of intracellular calcium, which could be another problem--even if you get a transcribing gene for luciferase spliced into the psilocybe genome, you may not have the genes or calcium stores needed to 'fire' the enzyme. e.g. it would glow but only as you mashed it up]. Could be completely wrong, I remember a photo of a tobacco plant that glowed continuously. . .was that luciferase? I haven't checked those links, will later.

Such things are extremely hard to do and your sample numbers are likely going to need to exceed a thousand or more. But you're right, you only need one to take :smile:


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Re: Glowing Shrooms-- Tek? [Re: Psilowarrior]
    #91074 -

I think it could be as simple as this. Obtain the bioluminescent gene. Getting it yourself from an organism would be extremely expensive as far as startup costs go to get the equipment plus your knowledge of biochemistry and metabolic pathways would have to be extensive, to say the least.

If you got it from a biochemistry company and used electroporation or whatever that's all it would take. It would be very easy if you could manufacture a plasmid that had a marker gene in it as well. (Make it immune to some fungicide, make it turn red, whatever) This would make the selection extremely simple since you would be looking for something easily identifiable. Better yet, watch for the mycelium colony that glows and make it real easy!!


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