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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90256 -

I have had that said so many times I can't count. Doesn't help when your friends all like to party. But there is paper trail with with rx drugs. Hey Moe did you get the PM I sent you? I would like to know what you think. I was thinking I could even send it to one of the spore vendors and they in turn could send it to the recipient if you all wanted to keep the winners address unknown.
Yes I deal drugs :-)

damn wish I could type

[This message has been edited by oDin (edited October 30, 1999).]


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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90257 -

Ok, so this has been done to shrooms and worked. But has it ever been done on Ergot fungus, Amanitas, or any Tryptamine-containing Plants?

[This message has been edited by PsiliPharm (edited November 08, 1999).]


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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90259 -

Hey Moe I sent you another PM. Today 11-9-99
I am trying to keep it a secret he he

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90260 -

Suggestion: Do this to all of the species listed in this thread, Other Tryptamine-containing Shrooms.

Also, any of the species' listed here in this post at Lycaeum's Main Forums in the Visionary Plants section:

(Almost) All Visionary Plants and Mushrooms

The above link may not work and you may have to log in to view it so go to the section and look for it if your interested.

-PsiliPharm

[This message has been edited by PsiliPharm (edited December 18, 1999).]


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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90261 -

Dr. Charles Lindeman is right.
Maybe you do not need to be chemist, but scientist definitely to do such thing.
Do not try this unless you are qualified.
And mycofile, thing about B+, where did you heard it? I am pretty interested in those real experiments done on psychoactive substances.

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90262 -

Dr. Charles Lindeman is right.
Maybe you do not need to be chemist, but scientist definitely to do such thing.
It is all project that has to be done properly if you want any even half success
Do not try this unless you are qualified.
And mycofile, thing about B+, where did you heard it? I am pretty interested in those real experiments done on psychoactive substances.

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90263 -

The thing about B+ made it's way around the web in the early days of the strain. The problem is that Mr G, the creator of the B+ (as well as the B the A and Ryche's TC among others) has disappeared. Apparently, nobody that ever had contact with him has heard from him. Or, maybe they are just protecting his privacy, in any case I can't get a hold of him. His old e-mail addy I had used in the past to chat with him is never answered, but the messages seem to be getting sent (hotmail usually cancels accounts if the mail isn't checked at least ocassionly). Who knows, perhaps is gone for good?

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90264 -

Thanks Mycofile

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90265 -

Colchicine has the remarkable property of doubling the chromosome number in a wide variety of plants when applied in concentrations of 0.1 to 0.3 percent.This drug interferes with spindle formation in mitosis.
Seed treatment(usually for 24 hours)produces tetraploidy in about 5 percent of the surviving seedlings.(1 out of 100 live)
It can be applied to the growing points of seedlings or large plant by a variety of methods.
Most valuable tool to overcome sterility assocaiated with interspecific hybrids and to transferring genes from other species,to creat a NEW specie!
aaron

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90266 -

aaron, how could you use colchicine to add genetic material from another species?

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90267 -

Ok, if you had 2 species that were incompatable with each other you could treat to induce tetraploid in both species and cross these 2 and produce a fertle aamphidloid. It would have the entire somatic complement of each species.
EXAMPLE:
Turnip x Kale = Rutabaga
another result:
A monoploid ( haploid of a diploid )can be created and used to restore fertility and could pass its genetics without altering the genitic make-up as much as a normal diploid to diploid cross.
Basically indiscriminate induction of tretraploid seldom leads to anything of immediate value, manupulations of ploidy for breeding purposes does.

aaron

[This message has been edited by aaron (edited December 12, 1999).]


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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90268 -

aaron, do you have any references for this process? I was going to provide a bunch of colchicine and polyploidy references but when I did press Submit it wouldn't work and I lost all of them. I'll have to go back and find them all again. Maybe later.

-PsiliPharm


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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90269 -

Here is some reference material to get you started.
Genetics and breeding of ebidle mushrooms / edited by Shu-Ting Chang,John A. Buswell,and Philip G. Miles.1993.

National Research council, 1984 Genetic engineering of plants.

U.S. Department of Agricilture. 1937. Better Plants and animals II.

North, C., 1979 Principles of crop improvement.

First and second edition of Frey,F.J.(ed),1981.Plant Breeding & Plant Breeding II.

Principles of Plant breeding. by R.W. Allard.

You'll need a good book on how to prepare microscope slides, tissue staining and cromozones counts.
good luck, and be careful

[This message has been edited by aaron (edited December 13, 1999).]


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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90270 -

I had a biology of cell class. And we teached
biology of animal and plant cell. And they have many differencess.
Mushrooms are different from those and I think, that all of you have to know much more about mushrooms to do anything, especially that serious

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90271 -

aaron, either in simpler terms, or more specifics. I don't see why the mycelium that was incompatible before doubling chromos would somehow become compatable just because you double the chromos. I mean, it certainly makes changes, but it's a pretty drastic statement to say that just because you have two different species with double chromos that they can suddenly mate. Polyploid orange trees don't breed well with polyploid strawberries for instance, and i think that is a good analogy.
More details if I missed something please, but I don't see why that would happen.
And crob, I sure hope your only reffering to the interbreeding of species when you say none of this can be done by any of us. Project Polyploid is underway and apparently showing progress, but the teks are secret pending results and dramatic fame with the inevitable success.

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90272 -

Mycof, I wish you luck

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90273 -

mycofile,
This is a complicated subject but here goes.
Your right an orange tree and a strawberry could never cross. The reason is they are in a separate genis. This will only work for organisims in the same species.
Example:
All oak trees are in the same species.
The reason why this works are not fully understood. It can be caused by just a single gene and when you double the genes to a tetraploid it will produce a homozygous condition, which allows mating to take place.
There is a bunch of reasons why they won't cross.
It's difficult to distinguish between genic and chromosomal sterility. But all you need to know is it should work if the individual that you double and it's cromozone split evenly it should be capable of crossing with another individual of the same species that has been doubled, these 2 species were incompatable before doubling. I wish you luck.
Aaron

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90274 -

I have created another thread on DMT World relating to this topic:

Using Colchicine to Produce Polyploidy DMT-Containing Plants!

And I've mentioned it here:

TMA production with Mescaline-containing Cactii

-PsiliPharm


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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90276 -

well guys im making the plunge, i think tomorrow im going to go try and find me some of those bulbs and do some experimenting, im thinking of adding the Cholcine to a couple of jars just too see what they do, im sure there is an annoying dog around here that i could use as a test subject...mwhahaha
but i dont think its as harmful as people think other wise bud growers wouldnt treat their plants with it all the time. Im thinking of trying to get some second generation of the treated mushrooms to use in testing. You just to be on the safe side, since mushrooms have the ability to pick up chemicals in the substrate, and they have a lot smaller yield as compared to someone growing cannibis treated plants

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Re: Genetically Mutated Mushrooms [Re: mycofile]
    #90277 -

Pure Colchicine can be obtained from many Chemical Supply companies on the internet just do a search with www.dogpile.com or something. I'll provide a list of companies pretty soon. I lost the list I was going to post, twice.

-PsiliPharm


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