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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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OPEN AIR INOCULATION METHOD.
    #5296250 -

OVEN TEK IS BOGUS.
(fact, heat convention draws in outside air)

Open air innoc method.

Get some STERILE gauze pads (small box-full is cheap).
(if need be use a folded paper towel, or any gauze pad)

Open a couple pads, pour on some 70% alc.

Pour some alc in a small dish, near you.
(for use in the process - later)

When you unsheathe a spore or LC syringe needle.

IMMEDIATELY wrap needle with alc soaked pad.

KEEP NEEDLE WRAPPED IN ALC PAD AT ALL TIMES.

Use other alc pad to swab lid.

Holding the needle in the pad.
(takes a little practice)

Place needle (enclosed in pad)over lid hole.

Press needle wrapped pad against lid.

Push needle into hole (with pad around it on outside of lid).

Push needle INTO lid hole.
(needle hub still surrounded by pad)

Squirt in inoculate.
(amount is up to you)

Withdraw needle, with pad surrounding needle.

Dip a pre-cut piece of micropore tape in alc dish.

Tape over injection hole with alc soaked micropore tape.
(it will stick, as alc evap's)

Proceed to next jar & repeat same process.

This innoc method minimizes needle exposure to OPEN AIR.


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Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296266 -

SO....no flame, all alcohol?


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Atheist Chat

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Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296268 -

Good post bro. I always use open air method. I just do it in a sterile bathroom. And you ask how is a bathroom sterile? ALOT of bleach, alcohol, and elbow grease,lol.

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Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296281 -

Scatmanrav recommend the alcohol soaked paper towel to me on my first grow and I have never done anything different. I do not feel that I have ever had contaminants introduced at inoculation.

My reasoning for this is because I have always had an entire batch fail or succeed. Leading me to believe the jar's where not properly sterile when inoculated. Or the syringe I used was DIRTY.

BTW
This is also done in an uncleaned (but not filthy) kitchen with windows open. Lately with the furnace on. I just make sure to knock them up right out of the PC.


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My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!

Edited by musher_420 (02/13/06 06:41 PM)

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Re: OPEN AIR INOCULATION METHOD. [Re: musher_420]
    #5296303 -

Well, I learned something from magash (i think). Just put a sponge thats been cut to the length of your needles, about 1in thick. You just keep those on your needle, soaked in alcohol. As you inject, the sponge withdraws and covers back as you pull out the needle.

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Re: OPEN AIR INOCULATION METHOD. [Re: musher_420]
    #5296305 -

I've been doing this since for a long time.

Works!

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Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296343 -

Nice Agar . I've been doing open air for months now with no problems . I do it the way you do , taking care of the syringe between inoculations , but do the jars a little different .



I put 2 1/4" holes in each lid . I stuff one with poly-fil and leave the other for gas exchage . When I'm ready to inoculate . i pick up a jar , tilt it on it's side and squirt alcohol from a squeeze bottle on the poly-fil . Give it a good shake to remove any excess alcohol and set it back down . Then I inoculate through the sterile poly-fil . I get 6 quarts ready at a time , inoculate those six , and get 6 more ready . I only do 6 at a time so the alcohol doesn't evaporate . It's like inoculating through an alcohol prep pad .

Peace,Love,Happiness and Harmony
:heart: Hippie Chick  :mushroom2:


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Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058

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Re: OPEN AIR INOCULATION METHOD. [Re: HippieChick]
    #5296362 -

Thats why I love self sealing injection sites.
For the newcomers...

Jar
RTV Silicone
Poly Tube
Polyfill

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Re: OPEN AIR INOCULATION METHOD. [Re: Hoss]
    #5296375 -

that sounds like a good F'in idea.
IME i dont get alot of contams from inoculating. and up until recently i only used spore syringes so my growth would be considerably slower than w/ LC.

don't get me wrong... this would probably cut my contam rate down from like 1 in 15 (give or take, depending) to non-existant.

this is why i'll be switching to RTV injection ports on my lids.

do you ever get a bit of substrate mess on the bottom of the sponge? i guess that question would only apply to PF jars...


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"DO NOT ADJUST YOUR MIND.  IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson

NO LEFT TURN UN-STONED

Edited by krill (02/13/06 06:55 PM)

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Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296378 -

agar said:
OVEN TEK IS BOGUS.
(fact, heat convention draws in outside air)



Yessiree :thumbup: :tongue:

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Re: OPEN AIR INOCULATION METHOD. [Re: krill]
    #5296385 -

Lol your right. that question would only apply to PF jars...

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Re: OPEN AIR INOCULATION METHOD. [Re: Snaggletooth]
    #5296405 -

Snaggletooth said:
SO....no flame, all alcohol?


I never flame needles.

If innoc solution is not contaminated, if needle is kept wrapped, if your procedure is tight, there is no need. :thumbup:

I used to flame agar innoc loops with this.

wickless alc lamp.


Now, I use this instead. :grin:
(bacticinerator)


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Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296456 -

WHAT"S HE GOT IN HIS BAG OF TRICKS THIS TIME? :smile:



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If your soul is sence this life is lost ...

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Re: OPEN AIR INOCULATION METHOD. [Re: lardnar]
    #5296484 -

DUTY-FREE CUSTOMS SHOPS ARE THE SHIT!!!
not trying to jack the thread... but...
i happen to know that the duty free customs shop between Vanccouver, B.C. and Washington Kick ASS!!! i got a handle of Jameson Irish Whisky (a personal fav) for like $18, a box of Glenfiddich chocolates for a few bucks, and they have REAL ABSINTHE (yes, WITH the Wormwood) for like $43 !!!


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"DO NOT ADJUST YOUR MIND.  IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson

NO LEFT TURN UN-STONED

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Re: OPEN AIR INOCULATION METHOD. [Re: lardnar]
    #5296531 -

lardnar said:
WHAT"S HE GOT IN HIS BAG OF TRICKS THIS TIME? :smile:




1200F inside that puppy.


Dental picks off ebay make good agar working tools.

Between plates, I sterilize the tool in the bacticinerator.

Wickless alc lamp is MUCH cheaper.
(can be had on ebay)


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Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296569 -

Hum...interesting, then I have to check out my tek then and give it a go  :thumbup:


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Atheist Chat

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Re: OPEN AIR INOCULATION METHOD. [Re: Snaggletooth]
    #5298328 -

Starting today... i have stoped using the oven tek.

But i don't think my issue is with inoculation. It's with sterilization. I am pc'n for 1 hr. and i think i need 1.5-2

so we'll see on todays run. i am doing 5 different tests.


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Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine

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Re: OPEN AIR INOCULATION METHOD. [Re: Oatman2000]
    #5689304 -

my method is a little different. I have a 3/16 hole covered with masking tape. I hole my syringe with the alcohol paper towel over it. I then take that towel off and wipe the tape on the jar and the tape flap. Then wipe needle and inject into the jar against the glass and rotate the jar to hit all sides. Then just push the tape down and recover the needle...repeat.

I think 1-100 contam


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First Grow No more carpet tek

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Re: OPEN AIR INOCULATION METHOD. [Re: Snaggletooth]
    #5689844 -

Snaggletooth said:
SO....no flame, all alcohol?


even with my hood i still preferr flaming my needles/tools.

see it glow, see it die :wink:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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Re: OPEN AIR INOCULATION METHOD. [Re: ohmatic]
    #5737366 -

THANKS THAT IS WHAT I FEEL LIKE GOING GETTING NU RUBBERMAIF SH*T YA KNOW AND START OVER MAYBE TAKE ABNOTHER HUNDERD AN GET THAT MUSHBOX GROW KIT WHO KNOW. THESE ARER THE MOST EXPENSIVE SHROOMS I HAVE EVER SEEN4

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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