Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
OPEN AIR INOCULATION METHOD.
    #5296250 - 02/13/06 06:34 PM (17 years, 11 months ago)

OVEN TEK IS BOGUS.
(fact, heat convention draws in outside air)

Open air innoc method.

Get some STERILE gauze pads (small box-full is cheap).
(if need be use a folded paper towel, or any gauze pad)

Open a couple pads, pour on some 70% alc.

Pour some alc in a small dish, near you.
(for use in the process - later)

When you unsheathe a spore or LC syringe needle.

IMMEDIATELY wrap needle with alc soaked pad.

KEEP NEEDLE WRAPPED IN ALC PAD AT ALL TIMES.

Use other alc pad to swab lid.

Holding the needle in the pad.
(takes a little practice)

Place needle (enclosed in pad)over lid hole.

Press needle wrapped pad against lid.

Push needle into hole (with pad around it on outside of lid).

Push needle INTO lid hole.
(needle hub still surrounded by pad)

Squirt in inoculate.
(amount is up to you)

Withdraw needle, with pad surrounding needle.

Dip a pre-cut piece of micropore tape in alc dish.

Tape over injection hole with alc soaked micropore tape.
(it will stick, as alc evap's)

Proceed to next jar & repeat same process.

This innoc method minimizes needle exposure to OPEN AIR.


--------------------


Extras: Filter Print Post Top
OfflineSnaggletooth
Stranger in a Strange Land
Male User Gallery

Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296266 - 02/13/06 06:37 PM (17 years, 11 months ago)

SO....no flame, all alcohol?


--------------------


Atheist Chat


Extras: Filter Print Post Top
InvisibleFungusMan
I81U812
Male User Gallery

Folding@home Statistics
Registered: 08/06/05
Posts: 3,112
Loc: Everywhere
Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296268 - 02/13/06 06:37 PM (17 years, 11 months ago)

Good post bro. I always use open air method. I just do it in a sterile bathroom. And you ask how is a bathroom sterile? ALOT of bleach, alcohol, and elbow grease,lol.


Extras: Filter Print Post Top
Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296281 - 02/13/06 06:39 PM (17 years, 11 months ago)

Scatmanrav recommend the alcohol soaked paper towel to me on my first grow and I have never done anything different. I do not feel that I have ever had contaminants introduced at inoculation.

My reasoning for this is because I have always had an entire batch fail or succeed. Leading me to believe the jar's where not properly sterile when inoculated. Or the syringe I used was DIRTY.

BTW
This is also done in an uncleaned (but not filthy) kitchen with windows open. Lately with the furnace on. I just make sure to knock them up right out of the PC.


--------------------
My Humidifier Reservoir Expansion Tek

Must read links for beginners

Btw- You are not what you own!


Edited by musher_420 (02/13/06 06:41 PM)


Extras: Filter Print Post Top
InvisibleFungusMan
I81U812
Male User Gallery

Folding@home Statistics
Registered: 08/06/05
Posts: 3,112
Loc: Everywhere
Re: OPEN AIR INOCULATION METHOD. [Re: musher_420]
    #5296303 - 02/13/06 06:43 PM (17 years, 11 months ago)

Well, I learned something from magash (i think). Just put a sponge thats been cut to the length of your needles, about 1in thick. You just keep those on your needle, soaked in alcohol. As you inject, the sponge withdraws and covers back as you pull out the needle.


Extras: Filter Print Post Top
OfflineHoss
Stranger
 User Gallery
Folding@home Statistics
Registered: 06/13/05
Posts: 297
Last seen: 12 years, 1 month
Re: OPEN AIR INOCULATION METHOD. [Re: musher_420]
    #5296305 - 02/13/06 06:43 PM (17 years, 11 months ago)

I've been doing this since for a long time.

Works!


Extras: Filter Print Post Top
OfflineHippieChick
Chicks can do it too!
Female User Gallery

Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 13 days
Trusted Cultivator
Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296343 - 02/13/06 06:49 PM (17 years, 11 months ago)

Nice Agar . I've been doing open air for months now with no problems . I do it the way you do , taking care of the syringe between inoculations , but do the jars a little different .



I put 2 1/4" holes in each lid . I stuff one with poly-fil and leave the other for gas exchage . When I'm ready to inoculate . i pick up a jar , tilt it on it's side and squirt alcohol from a squeeze bottle on the poly-fil . Give it a good shake to remove any excess alcohol and set it back down . Then I inoculate through the sterile poly-fil . I get 6 quarts ready at a time , inoculate those six , and get 6 more ready . I only do 6 at a time so the alcohol doesn't evaporate . It's like inoculating through an alcohol prep pad .

Peace,Love,Happiness and Harmony
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


Extras: Filter Print Post Top
InvisibleFungusMan
I81U812
Male User Gallery

Folding@home Statistics
Registered: 08/06/05
Posts: 3,112
Loc: Everywhere
Re: OPEN AIR INOCULATION METHOD. [Re: HippieChick]
    #5296362 - 02/13/06 06:51 PM (17 years, 11 months ago)

Thats why I love self sealing injection sites.
For the newcomers...

Jar
RTV Silicone
Poly Tube
Polyfill


Extras: Filter Print Post Top
Offlinekrill
absolute gonzo
Male

Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
Re: OPEN AIR INOCULATION METHOD. [Re: Hoss]
    #5296375 - 02/13/06 06:54 PM (17 years, 11 months ago)

that sounds like a good F'in idea.
IME i dont get alot of contams from inoculating. and up until recently i only used spore syringes so my growth would be considerably slower than w/ LC.

don't get me wrong... this would probably cut my contam rate down from like 1 in 15 (give or take, depending) to non-existant.

this is why i'll be switching to RTV injection ports on my lids.

do you ever get a bit of substrate mess on the bottom of the sponge? i guess that question would only apply to PF jars...


--------------------
"DO NOT ADJUST YOUR MIND.  IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson

NO LEFT TURN UN-STONED


Edited by krill (02/13/06 06:55 PM)


Extras: Filter Print Post Top
InvisibleMrMaddHatter
Dementia praecox
 User Gallery

Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296378 - 02/13/06 06:55 PM (17 years, 11 months ago)

Quote:

agar said:
OVEN TEK IS BOGUS.
(fact, heat convention draws in outside air)





Yessiree :thumbup: :tongue:


Extras: Filter Print Post Top
InvisibleFungusMan
I81U812
Male User Gallery

Folding@home Statistics
Registered: 08/06/05
Posts: 3,112
Loc: Everywhere
Re: OPEN AIR INOCULATION METHOD. [Re: krill]
    #5296385 - 02/13/06 06:56 PM (17 years, 11 months ago)

Lol your right. that question would only apply to PF jars...


Extras: Filter Print Post Top
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: OPEN AIR INOCULATION METHOD. [Re: Snaggletooth]
    #5296405 - 02/13/06 06:59 PM (17 years, 11 months ago)

Quote:

Snaggletooth said:
SO....no flame, all alcohol?




I never flame needles.

If innoc solution is not contaminated, if needle is kept wrapped, if your procedure is tight, there is no need. :thumbup:

I used to flame agar innoc loops with this.

wickless alc lamp.


Now, I use this instead. :grin:
(bacticinerator)


--------------------


Extras: Filter Print Post Top
Offlinelardnar
Pu Pu Platter
Male

Registered: 06/15/05
Posts: 703
Loc: Behind what appears
Last seen: 16 years, 11 months
Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296456 - 02/13/06 07:09 PM (17 years, 11 months ago)

WHAT"S HE GOT IN HIS BAG OF TRICKS THIS TIME? :smile:



--------------------
If your soul is sence this life is lost ...


Extras: Filter Print Post Top
Offlinekrill
absolute gonzo
Male

Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
Re: OPEN AIR INOCULATION METHOD. [Re: lardnar]
    #5296484 - 02/13/06 07:13 PM (17 years, 11 months ago)

DUTY-FREE CUSTOMS SHOPS ARE THE SHIT!!!
not trying to jack the thread... but...
i happen to know that the duty free customs shop between Vanccouver, B.C. and Washington Kick ASS!!! i got a handle of Jameson Irish Whisky (a personal fav) for like $18, a box of Glenfiddich chocolates for a few bucks, and they have REAL ABSINTHE (yes, WITH the Wormwood) for like $43 !!!


--------------------
"DO NOT ADJUST YOUR MIND.  IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson

NO LEFT TURN UN-STONED


Extras: Filter Print Post Top
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: OPEN AIR INOCULATION METHOD. [Re: lardnar]
    #5296531 - 02/13/06 07:21 PM (17 years, 11 months ago)

Quote:

lardnar said:
WHAT"S HE GOT IN HIS BAG OF TRICKS THIS TIME? :smile:






1200F inside that puppy.


Dental picks off ebay make good agar working tools.

Between plates, I sterilize the tool in the bacticinerator.

Wickless alc lamp is MUCH cheaper.
(can be had on ebay)


--------------------


Extras: Filter Print Post Top
OfflineSnaggletooth
Stranger in a Strange Land
Male User Gallery

Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5296569 - 02/13/06 07:28 PM (17 years, 11 months ago)

Hum...interesting, then I have to check out my tek then and give it a go  :thumbup:


--------------------


Atheist Chat


Extras: Filter Print Post Top
OfflineOatman2000
-=Outa Space=-
Male User Gallery

Folding@home Statistics
Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
Trusted Cultivator
Re: OPEN AIR INOCULATION METHOD. [Re: Snaggletooth]
    #5298328 - 02/14/06 07:44 AM (17 years, 11 months ago)

Starting today... i have stoped using the oven tek.

But i don't think my issue is with inoculation. It's with sterilization. I am pc'n for 1 hr. and i think i need 1.5-2

so we'll see on todays run. i am doing 5 different tests.


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


Extras: Filter Print Post Top
OfflineJSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 2 months, 15 days
Re: OPEN AIR INOCULATION METHOD. [Re: Oatman2000]
    #5689304 - 05/30/06 12:48 AM (17 years, 8 months ago)

my method is a little different. I have a 3/16 hole covered with masking tape. I hole my syringe with the alcohol paper towel over it. I then take that towel off and wipe the tape on the jar and the tape flap. Then wipe needle and inject into the jar against the glass and rotate the jar to hit all sides. Then just push the tape down and recover the needle...repeat.

I think 1-100 contam


--------------------
First Grow No more carpet tek


Extras: Filter Print Post Top
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: OPEN AIR INOCULATION METHOD. [Re: Snaggletooth]
    #5689844 - 05/30/06 07:10 AM (17 years, 7 months ago)

Quote:

Snaggletooth said:
SO....no flame, all alcohol?




even with my hood i still preferr flaming my needles/tools.

see it glow, see it die :wink:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Extras: Filter Print Post Top
Offlinegarygenn69
Stranger
Male User Gallery
Registered: 01/25/06
Posts: 8
Last seen: 14 years, 7 months
Re: OPEN AIR INOCULATION METHOD. [Re: ohmatic]
    #5737366 - 06/11/06 09:34 AM (17 years, 7 months ago)

THANKS THAT IS WHAT I FEEL LIKE GOING GETTING NU RUBBERMAIF SH*T YA KNOW AND START OVER MAYBE TAKE ABNOTHER HUNDERD AN GET THAT MUSHBOX GROW KIT WHO KNOW. THESE ARER THE MOST EXPENSIVE SHROOMS I HAVE EVER SEEN4


Extras: Filter Print Post Top
InvisiblePremedman1
Assistant to the insistent
 User Gallery

Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
Trusted Cultivator
Re: OPEN AIR INOCULATION METHOD. [Re: ohmatic]
    #5737374 - 06/11/06 09:43 AM (17 years, 7 months ago)

Quote:

ohmatic said:

even with my hood i still preferr flaming my needles/tools.

see it glow, see it die :wink:




:smile::thumbup:


--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.


Extras: Filter Print Post Top
OfflineNinjaGaiden
Wizard of Odd
Registered: 01/06/04
Posts: 27
Loc: Florida
Last seen: 17 years, 5 months
Re: OPEN AIR INOCULATION METHOD. [Re: agar]
    #5740665 - 06/12/06 03:13 AM (17 years, 7 months ago)

Quote:

agar said:
OVEN TEK IS BOGUS.
(fact, heat convention draws in outside air)





I've had 100% success with using the oven tek. I do everything: Make syringes, inoculate jars, G2G transfers using the oven.

I'm not at all trying to debate if the tek itself is bogus or not, but it does work for some.


--------------------
Can't you trip like I do?


Extras: Filter Print Post Top
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: OPEN AIR INOCULATION METHOD. [Re: FungusMan]
    #5740679 - 06/12/06 03:28 AM (17 years, 7 months ago)

Quote:

FungusMan said:
Well, I learned something from magash (i think). Just put a sponge thats been cut to the length of your needles, about 1in thick. You just keep those on your needle, soaked in alcohol. As you inject, the sponge withdraws and covers back as you pull out the needle.





Wow! That's a great tip.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid


Extras: Filter Print Post Top
InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: OPEN AIR INOCULATION METHOD. [Re: NinjaGaiden]
    #5740691 - 06/12/06 03:42 AM (17 years, 7 months ago)

Quote:

NinjaGaiden said:

Quote:

agar said:

OVEN TEK IS BOGUS.
(fact, heat convention draws in outside air)





I've had 100% success with using the oven tek. I do everything: Make syringes, inoculate jars, G2G transfers using the oven.

I'm not at all trying to debate if the tek itself is bogus or not, but it does work for some.





more like 100% LUCK

and I will debate it...

the Oven tek is crap!~


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Extras: Filter Print Post Top
OfflineNinjaGaiden
Wizard of Odd
Registered: 01/06/04
Posts: 27
Loc: Florida
Last seen: 17 years, 5 months
Re: OPEN AIR INOCULATION METHOD. [Re: Roadkill]
    #5740773 - 06/12/06 05:24 AM (17 years, 7 months ago)

Quote:


more like 100% LUCK

and I will debate it...

the Oven tek is crap!~





If you say so...

I've done tons of open air g2g over the oven without 1 contam yet.

Move two feet up to my counter, try the same thing, and I get contams every single time.

...Luck?


--------------------
Can't you trip like I do?


Extras: Filter Print Post Top
OfflineAscension
Stranger

Registered: 01/13/05
Posts: 156
Loc: Still trying to work it o...
Last seen: 17 years, 7 months
Re: OPEN AIR INOCULATION METHOD. [Re: NinjaGaiden]
    #5740793 - 06/12/06 06:02 AM (17 years, 7 months ago)

I have ALWAYS done everything in open air.

When I was studying biology at uni, when we use to do lab work like streaking of cultures onto agar plates, everything was always done in open air.

We would just sterelize the inoculation loop in a flame, dip it into the liquid culture and then streak it along the agar plate which would be open to the air.

Never got contams, I believe all this fume hood stuff is just being over carefull, no need for it in my opinion.

I do EVERYTHING in open, making syringes etc, the only thing I cover up are when im making spore prints as they have to sit for a while.


Extras: Filter Print Post Top
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: OPEN AIR INOCULATION METHOD. [Re: Ascension]
    #5740889 - 06/12/06 07:52 AM (17 years, 7 months ago)

That's using a bunsen burner. It creates a small clean zone around it. Not quite the same as just doing things in open air.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid


Extras: Filter Print Post Top
OfflineKaptKid
Spaced Pirate
Male User Gallery

Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 3 years, 11 months
Re: OPEN AIR INOCULATION METHOD. [Re: Roadkill]
    #5740935 - 06/12/06 08:28 AM (17 years, 7 months ago)

Quote:

Roadkill said:
Quote:

NinjaGaiden said:

Quote:

agar said:

OVEN TEK IS BOGUS.
(fact, heat convention draws in outside air)





I've had 100% success with using the oven tek.  I do everything: Make syringes, inoculate jars, G2G transfers using the oven. 

I'm not at all trying to debate if the tek itself is bogus or not, but it does work for some.





more like 100% LUCK

and I will debate it...

the Oven tek is crap!~


tc




I would think some where in all that turbulence is a safe place to work. It would take a lot of luck to stay in it all the time. If one had that kind of luck they would be better off in a casino.


:sun:


--------------------
Child of the 60's, Tripping ever since.


:sun:


Extras: Filter Print Post Top
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: OPEN AIR INOCULATION METHOD. [Re: KaptKid]
    #5740968 - 06/12/06 08:41 AM (17 years, 7 months ago)

Contams fall downward and land on your surfaces. So if you stay in an updraft of warm air it probably does help. If all the air is moving upwards then nothing can fall into your open jar or petri dish.

I wouldn't trust it as a method though. But it might help a little if the rest of your sterile technique is good.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid


Extras: Filter Print Post Top
InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: OPEN AIR INOCULATION METHOD. [Re: fastfred]
    #5741079 - 06/12/06 09:24 AM (17 years, 7 months ago)

Convection from a source of heat will move airborne mold spores upward and outward from the source of heat. Examples are hot air rising from a stove top burner, oven heating element, gas grill, Bunsen burner, or even something so small as a lit match.

Household air may have as many as 10,000 invisible particulates per cubic foot floating in it, many of which are contaminates. The laws governing thermodynamic air movement preclude any argument otherwise.


--------------------
Perfection is attained by slow degrees; it requires the hand of time...
[


Extras: Filter Print Post Top
InvisiblePsychoslut
The Mother Fucking Bear-o-dactyl
 User Gallery
Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
Re: OPEN AIR INOCULATION METHOD. [Re: HippieChick]
    #5741140 - 06/12/06 10:00 AM (17 years, 7 months ago)

Yeah hippiechick I do the same thing. I do all my inoculations in open air and all i do is flame the needle for a few seconds between inoc. I just pull my tape back, inject, and tape back over.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


Extras: Filter Print Post Top
InvisibleFooMan
 User Gallery

Folding@home Statistics
Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
Trusted Cultivator
Re: OPEN AIR INOCULATION METHOD. [Re: Ascension]
    #5741264 - 06/12/06 10:43 AM (17 years, 7 months ago)

Quote:

Ascension said:
I have ALWAYS done everything in open air.

When I was studying biology at uni, when we use to do lab work like streaking of cultures onto agar plates, everything was always done in open air.

We would just sterelize the inoculation loop in a flame, dip it into the liquid culture and then streak it along the agar plate which would be open to the air.

Never got contams, I believe all this fume hood stuff is just being over carefull, no need for it in my opinion.

I do EVERYTHING in open, making syringes etc, the only thing I cover up are when im making spore prints as they have to sit for a while.




Do you live in a bubble? Advising people to do agar or any other type of sterile work in open air is just ridiculous. Any serious mycologist knows better.


--------------------

Quick WBS Prep


Extras: Filter Print Post Top
InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: OPEN AIR INOCULATION METHOD. [Re: NinjaGaiden]
    #5741345 - 06/12/06 11:14 AM (17 years, 7 months ago)

using the oven tek...


example by Anno...




wanna test it yourself?

use a lit cigarette, cigar, joint, or a stick of incense.

and hold the object lower than the lip of the jar.

and see where the smoke goes.

that will be the air flow movement around your jar and work space.



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Extras: Filter Print Post Top
OfflineCryogenicz
what?
Male User Gallery

Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 8 months
Re: OPEN AIR INOCULATION METHOD. [Re: FooMan]
    #5741361 - 06/12/06 11:22 AM (17 years, 7 months ago)

this cracks me up... Nothing is Sterile around us, unless its been filtered or clean... WE MUST modify our environment in order to keep our cultures clean. Every mushroom spawn company uses flow hoods for agar work. There is no point lowering your success rate any lower than 97%. Not to mention, contams can hide in spores/mycelium without detection for a while, in the meantime they are building up momentum. Answer this - Why risk it?


--------------------
www.MycoPath.com
Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more!
Mushroom Supplies. Fast Turnaround Times. Great Service.
orders@mycopath.com
enter code shroomery for 10% off product.

www.FungiForum.com


Extras: Filter Print Post Top
OfflineAsc1189
The New Guy

Registered: 11/18/05
Posts: 62
Last seen: 12 years, 10 months
Re: OPEN AIR INOCULATION METHOD. [Re: Roadkill]
    #5741403 - 06/12/06 11:32 AM (17 years, 7 months ago)

Good idea to bring that up. So many people have different ways of innoculating and Ive been doing this process for a long time and it seems to cut down on contaim. issues. I give 10 out 10 for this one.


Extras: Filter Print Post Top
InvisibleFooMan
 User Gallery

Folding@home Statistics
Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
Trusted Cultivator
Re: OPEN AIR INOCULATION METHOD. [Re: Cryogenicz]
    #5741436 - 06/12/06 11:41 AM (17 years, 7 months ago)

Quote:

Cryogenicz said:
this cracks me up... Nothing is Sterile around us, unless its been filtered or clean... WE MUST modify our environment in order to keep our cultures clean. Every mushroom spawn company uses flow hoods for agar work. There is no point lowering your success rate any lower than 97%. Not to mention, contams can hide in spores/mycelium without detection for a while, in the meantime they are building up momentum. Answer this - Why risk it?




I wouldn't risk it. The person I originally quoted apparently would.


--------------------

Quick WBS Prep


Extras: Filter Print Post Top
Offline76degrees
Apprentice

Registered: 06/08/06
Posts: 350
Loc: Middle Earth
Last seen: 17 years, 4 months
Re: OPEN AIR INOCULATION METHOD. [Re: FungusMan]
    #5756522 - 06/16/06 08:14 AM (17 years, 7 months ago)

That's a great idea.


--------------------
The world is changed. I feel it in the water. I feel it in the earth. I smell it in the air. Much that once was is lost, for none now live who remember it.


Extras: Filter Print Post Top
Offlinetallgreen
chillin like avillain
 User Gallery
Registered: 05/21/06
Posts: 293
Last seen: 17 years, 4 months
Re: OPEN AIR INOCULATION METHOD. [Re: 76degrees]
    #5756704 - 06/16/06 09:43 AM (17 years, 7 months ago)

I take cheap plastic tarps and tape them to the ceiling in the kitchen. I then spray sterilize the entire inside before doing anything. It's a mini makeshift clean-room. Zero air movement. Anytime air moves around the plastic moves, so when the plastic is dead still you can bet the air is also.


--------------------
Nothing you can know that isn't known.
Nothing you can see that isn't shown.
Nowhere you can be that isn't where you're meant to be.
It's easy.
All you need is love.
- The Beatles


Extras: Filter Print Post Top
InvisiblePeterthinks
(Caulking) gun for hire


Registered: 11/10/04
Posts: 2,379
Re: OPEN AIR INOCULATION METHOD. [Re: garygenn69]
    #7398053 - 09/11/07 07:30 PM (16 years, 4 months ago)

sometimes I use a propane torch like they use to solder copper plumbing pipe together, I just let it run on low while I work.to sterilize anything just hold it up to the flame.
lately I just leave the blade on the stove element as it's on high and it works very well.convenient since I do a lot of my work on the kitchen counter.
:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


Extras: Filter Print Post Top
OfflineDr_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 06/03/07
Posts: 1,839
Loc: Colorado
Last seen: 16 years, 4 months
Re: OPEN AIR INOCULATION METHOD. [Re: Roadkill]
    #7398535 - 09/11/07 09:14 PM (16 years, 4 months ago)

Quote:

Roadkill said:
and I will debate it...

the Oven tek is crap!~


tc




The inside of my oven is the most sterile and spore-free place in my apartment. I prefer to use techniques that don't require opening the jars at all- but when I have to open one, I do it inside the oven.

Someday I'll build a glovebox- but not today.


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* Birdseed inoculation method? nomez_ 2,608 16 02/06/03 07:35 AM
by fugu
* inoculating birdeed needle wont reach sides Northernsoul 1,102 5 01/25/02 01:26 AM
by Northernsoul
* flaming needles archnut 1,103 2 05/20/02 10:30 AM
by baraka
* sterlizing needles tomatofarmer 1,094 1 01/11/02 11:23 AM
by Mordor
* Will alcohol kill spores? Visigoth 3,310 2 03/27/02 05:21 PM
by WakingUpLate
* Can i dissenfect the syringe with lysol spray? Artifice 1,037 6 06/23/03 11:50 PM
by chineseguy
* Easy to make Inoculation hood lysergic 6,542 11 07/10/03 03:58 PM
by lysergic
* Oh shit.... fire in the inoculation room. Burning alcohol none 849 3 08/03/03 03:58 PM
by shirley knott

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
20,449 topic views. 18 members, 159 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.008 seconds on 14 queries.