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Shop: MushCult - Live Mushroom Cultures Black morel (Morchella importuna) live culture on agar   Amazon ½ Pint Jars

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Offlinearchnut
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Registered: 05/14/02
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flaming needles
    #637290 - 05/20/02 08:06 AM (16 years, 6 months ago)

when innoculating i use a plastic psuedo glove box. is flaming the needle after each hole ( PF method ) a must. if so how will i know when the needle i cooled so the heat does not kill the spores? any suggestions would be great....thanks


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OfflineBruiser
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Re: flaming needles [Re: archnut]
    #637396 - 05/20/02 09:26 AM (16 years, 6 months ago)

You can flame sterilize the needle before each jar, and wipe the needle with alcohol between each innoculation hole. Right after you flame the needle, you can wipe it down with alcohol to help cool it and to wipe off any of the black residue from flaming.
-Bruiser


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Offlinebaraka
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Re: flaming needles [Re: Bruiser]
    #637694 - 05/20/02 12:30 PM (16 years, 6 months ago)

Needle flaming is overboard. You can get away with just wiping it down with a cotton ball soaken in 70% iso.


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Shop: MushCult - Live Mushroom Cultures Black morel (Morchella importuna) live culture on agar   Amazon ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Contamination Forum

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