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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Premedman1
Assistant to the insistent


Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Re: OPEN AIR INOCULATION METHOD. [Re: ohmatic]
#5737374 - 06/11/06 09:43 AM (17 years, 7 months ago) |
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Quote:
ohmatic said:
even with my hood i still preferr flaming my needles/tools.
see it glow, see it die

-------------------- Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
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NinjaGaiden
Wizard of Odd
Registered: 01/06/04
Posts: 27
Loc: Florida
Last seen: 17 years, 5 months
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Re: OPEN AIR INOCULATION METHOD. [Re: agar]
#5740665 - 06/12/06 03:13 AM (17 years, 7 months ago) |
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Quote:
agar said: OVEN TEK IS BOGUS. (fact, heat convention draws in outside air)
I've had 100% success with using the oven tek. I do everything: Make syringes, inoculate jars, G2G transfers using the oven.
I'm not at all trying to debate if the tek itself is bogus or not, but it does work for some.
-------------------- Can't you trip like I do?
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: OPEN AIR INOCULATION METHOD. [Re: FungusMan]
#5740679 - 06/12/06 03:28 AM (17 years, 7 months ago) |
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Quote:
FungusMan said: Well, I learned something from magash (i think). Just put a sponge thats been cut to the length of your needles, about 1in thick. You just keep those on your needle, soaked in alcohol. As you inject, the sponge withdraws and covers back as you pull out the needle.
Wow! That's a great tip.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: OPEN AIR INOCULATION METHOD. [Re: NinjaGaiden]
#5740691 - 06/12/06 03:42 AM (17 years, 7 months ago) |
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Quote:
NinjaGaiden said:
Quote:
agar said:
OVEN TEK IS BOGUS. (fact, heat convention draws in outside air)
I've had 100% success with using the oven tek. I do everything: Make syringes, inoculate jars, G2G transfers using the oven.
I'm not at all trying to debate if the tek itself is bogus or not, but it does work for some.
more like 100% LUCK
and I will debate it...
the Oven tek is crap!~
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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NinjaGaiden
Wizard of Odd
Registered: 01/06/04
Posts: 27
Loc: Florida
Last seen: 17 years, 5 months
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Re: OPEN AIR INOCULATION METHOD. [Re: Roadkill]
#5740773 - 06/12/06 05:24 AM (17 years, 7 months ago) |
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Quote:
more like 100% LUCK
and I will debate it...
the Oven tek is crap!~
If you say so...
I've done tons of open air g2g over the oven without 1 contam yet.
Move two feet up to my counter, try the same thing, and I get contams every single time.
...Luck?
-------------------- Can't you trip like I do?
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Ascension
Stranger

Registered: 01/13/05
Posts: 156
Loc: Still trying to work it o...
Last seen: 17 years, 7 months
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Re: OPEN AIR INOCULATION METHOD. [Re: NinjaGaiden]
#5740793 - 06/12/06 06:02 AM (17 years, 7 months ago) |
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I have ALWAYS done everything in open air.
When I was studying biology at uni, when we use to do lab work like streaking of cultures onto agar plates, everything was always done in open air.
We would just sterelize the inoculation loop in a flame, dip it into the liquid culture and then streak it along the agar plate which would be open to the air.
Never got contams, I believe all this fume hood stuff is just being over carefull, no need for it in my opinion.
I do EVERYTHING in open, making syringes etc, the only thing I cover up are when im making spore prints as they have to sit for a while.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: OPEN AIR INOCULATION METHOD. [Re: Ascension]
#5740889 - 06/12/06 07:52 AM (17 years, 7 months ago) |
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That's using a bunsen burner. It creates a small clean zone around it. Not quite the same as just doing things in open air.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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KaptKid
Spaced Pirate


Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 3 years, 11 months
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Re: OPEN AIR INOCULATION METHOD. [Re: Roadkill]
#5740935 - 06/12/06 08:28 AM (17 years, 7 months ago) |
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Quote:
Roadkill said:
Quote:
NinjaGaiden said:
Quote:
agar said:
OVEN TEK IS BOGUS. (fact, heat convention draws in outside air)
I've had 100% success with using the oven tek. I do everything: Make syringes, inoculate jars, G2G transfers using the oven.
I'm not at all trying to debate if the tek itself is bogus or not, but it does work for some.
more like 100% LUCK
and I will debate it...
the Oven tek is crap!~
tc
I would think some where in all that turbulence is a safe place to work. It would take a lot of luck to stay in it all the time. If one had that kind of luck they would be better off in a casino.
-------------------- Child of the 60's, Tripping ever since.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: OPEN AIR INOCULATION METHOD. [Re: KaptKid]
#5740968 - 06/12/06 08:41 AM (17 years, 7 months ago) |
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Contams fall downward and land on your surfaces. So if you stay in an updraft of warm air it probably does help. If all the air is moving upwards then nothing can fall into your open jar or petri dish.
I wouldn't trust it as a method though. But it might help a little if the rest of your sterile technique is good.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: OPEN AIR INOCULATION METHOD. [Re: fastfred]
#5741079 - 06/12/06 09:24 AM (17 years, 7 months ago) |
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Convection from a source of heat will move airborne mold spores upward and outward from the source of heat. Examples are hot air rising from a stove top burner, oven heating element, gas grill, Bunsen burner, or even something so small as a lit match.
Household air may have as many as 10,000 invisible particulates per cubic foot floating in it, many of which are contaminates. The laws governing thermodynamic air movement preclude any argument otherwise.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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Psychoslut
The Mother Fucking Bear-o-dactyl

Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
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Re: OPEN AIR INOCULATION METHOD. [Re: HippieChick]
#5741140 - 06/12/06 10:00 AM (17 years, 7 months ago) |
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Yeah hippiechick I do the same thing. I do all my inoculations in open air and all i do is flame the needle for a few seconds between inoc. I just pull my tape back, inject, and tape back over.
--------------------
[quote]KristiMidocean said: Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: OPEN AIR INOCULATION METHOD. [Re: Ascension]
#5741264 - 06/12/06 10:43 AM (17 years, 7 months ago) |
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Quote:
Ascension said: I have ALWAYS done everything in open air.
When I was studying biology at uni, when we use to do lab work like streaking of cultures onto agar plates, everything was always done in open air.
We would just sterelize the inoculation loop in a flame, dip it into the liquid culture and then streak it along the agar plate which would be open to the air.
Never got contams, I believe all this fume hood stuff is just being over carefull, no need for it in my opinion.
I do EVERYTHING in open, making syringes etc, the only thing I cover up are when im making spore prints as they have to sit for a while.
Do you live in a bubble? Advising people to do agar or any other type of sterile work in open air is just ridiculous. Any serious mycologist knows better.
--------------------
Quick WBS Prep
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: OPEN AIR INOCULATION METHOD. [Re: NinjaGaiden]
#5741345 - 06/12/06 11:14 AM (17 years, 7 months ago) |
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using the oven tek...
example by Anno...
wanna test it yourself?
use a lit cigarette, cigar, joint, or a stick of incense.
and hold the object lower than the lip of the jar.
and see where the smoke goes.
that will be the air flow movement around your jar and work space.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Cryogenicz
what?


Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 8 months
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Re: OPEN AIR INOCULATION METHOD. [Re: FooMan]
#5741361 - 06/12/06 11:22 AM (17 years, 7 months ago) |
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this cracks me up... Nothing is Sterile around us, unless its been filtered or clean... WE MUST modify our environment in order to keep our cultures clean. Every mushroom spawn company uses flow hoods for agar work. There is no point lowering your success rate any lower than 97%. Not to mention, contams can hide in spores/mycelium without detection for a while, in the meantime they are building up momentum. Answer this - Why risk it?
-------------------- www.MycoPath.com Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more! Mushroom Supplies. Fast Turnaround Times. Great Service. orders@mycopath.com enter code shroomery for 10% off product. www.FungiForum.com
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Asc1189
The New Guy

Registered: 11/18/05
Posts: 62
Last seen: 12 years, 10 months
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Re: OPEN AIR INOCULATION METHOD. [Re: Roadkill]
#5741403 - 06/12/06 11:32 AM (17 years, 7 months ago) |
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Good idea to bring that up. So many people have different ways of innoculating and Ive been doing this process for a long time and it seems to cut down on contaim. issues. I give 10 out 10 for this one.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: OPEN AIR INOCULATION METHOD. [Re: Cryogenicz]
#5741436 - 06/12/06 11:41 AM (17 years, 7 months ago) |
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Quote:
Cryogenicz said: this cracks me up... Nothing is Sterile around us, unless its been filtered or clean... WE MUST modify our environment in order to keep our cultures clean. Every mushroom spawn company uses flow hoods for agar work. There is no point lowering your success rate any lower than 97%. Not to mention, contams can hide in spores/mycelium without detection for a while, in the meantime they are building up momentum. Answer this - Why risk it?
I wouldn't risk it. The person I originally quoted apparently would.
--------------------
Quick WBS Prep
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76degrees
Apprentice

Registered: 06/08/06
Posts: 350
Loc: Middle Earth
Last seen: 17 years, 4 months
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Re: OPEN AIR INOCULATION METHOD. [Re: FungusMan]
#5756522 - 06/16/06 08:14 AM (17 years, 7 months ago) |
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That's a great idea.
-------------------- The world is changed. I feel it in the water. I feel it in the earth. I smell it in the air. Much that once was is lost, for none now live who remember it.
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tallgreen
chillin like avillain

Registered: 05/21/06
Posts: 293
Last seen: 17 years, 4 months
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Re: OPEN AIR INOCULATION METHOD. [Re: 76degrees]
#5756704 - 06/16/06 09:43 AM (17 years, 7 months ago) |
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I take cheap plastic tarps and tape them to the ceiling in the kitchen. I then spray sterilize the entire inside before doing anything. It's a mini makeshift clean-room. Zero air movement. Anytime air moves around the plastic moves, so when the plastic is dead still you can bet the air is also.
-------------------- Nothing you can know that isn't known. Nothing you can see that isn't shown. Nowhere you can be that isn't where you're meant to be. It's easy. All you need is love. - The Beatles
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Peterthinks
(Caulking) gun for hire


Registered: 11/10/04
Posts: 2,379
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Re: OPEN AIR INOCULATION METHOD. [Re: garygenn69]
#7398053 - 09/11/07 07:30 PM (16 years, 4 months ago) |
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sometimes I use a propane torch like they use to solder copper plumbing pipe together, I just let it run on low while I work.to sterilize anything just hold it up to the flame. lately I just leave the blade on the stove element as it's on high and it works very well.convenient since I do a lot of my work on the kitchen counter.
-------------------- Give a man a fire and he will be warm for the rest of the night. Set a man on fire and he will be warm for the rest of his life. NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS? Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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Dr_T
Random Dude




Registered: 06/03/07
Posts: 1,839
Loc: Colorado
Last seen: 16 years, 4 months
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Re: OPEN AIR INOCULATION METHOD. [Re: Roadkill]
#7398535 - 09/11/07 09:14 PM (16 years, 4 months ago) |
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Quote:
Roadkill said: and I will debate it...
the Oven tek is crap!~
tc
The inside of my oven is the most sterile and spore-free place in my apartment. I prefer to use techniques that don't require opening the jars at all- but when I have to open one, I do it inside the oven.
Someday I'll build a glovebox- but not today.
-------------------- Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.
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