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Amazon Shop for: ½ Pint Jars, HEPA Filter, Laminar Flow Hood, Pressure Cooker, Rye Grain

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InvisibleLanaV
Head Banana
Female

Registered: 10/28/99
Posts: 3,086
Loc: www.MycoSupply.com
Old Rye Tek being brought back up...
    #350653 - 06/28/01 11:21 AM (15 years, 9 months ago)

Hi,
This is an old post that for some reason I can't find. I had a copy of it and thought that some of you may find it interesting. Rye is fun! The info is by Ripper (who isn't around anymore:( and I'd like to hear what a few of you think about it.

Here it is,
Lana

-----------------------------------------------------------------------Author Topic: Tips on Rye Jars that made me succesful. Ripper
Junior Member
(Total posts: 5)
posted January 20, 2000 08:53 AM

Well first and foremost I'd like to say that I'm VERY eager to share knowledge. As far as rye cultivation goes here's my two cents. I don't use a flow hood or for that matter a HEPA filter at all throughout the entire proccess and have had 0% contams since getting my method down good. Lets start out with the basics..

Materials Required

Rye Grain (Preferably from Fungi Perfecti since their grain is of known quality and is grown organicly)
1 Quart Regular Mouth Canning Jars(I use the Ball brand... Kerr would prolly work fine also)
Synthetic Filter Discs (Obtained from Fungi Perfecti... PLEASE BE WARNED! I have used filter discs from other suppliers and they aren't the SAME! Fungi Perfecti's filter discs are thicker and can withstand much more abuse, re-using the others isn't really feasible.. And as far as making your own filter discs from vacuum cleaner bags or this that or the other.. Well I just see it was $.45 a disc isn't alot to pay for piece of mind and a perfect fit)
Distilled Water (this is important not for its sterility but rather because it doesn't leave nasty mineral deposits in jars/pressure cooker)
Pressure Cooker (I use a Mirro 8 Quart, it holds 4 quart jars at once)
The Process(Please note: There is a 24 hour waiting period between preparing the jars at pressure cooking them, so please be prepared)

Day 1
Get your jars outta the box, you don't need the lids, just the retaining rings.. now take your jars and set them out on your counter.. Being sterile isn't really important at this point... Fill each jar with 1 cup of rye, just take a 1 cup measuring cup and dip it in the bag, level it off, this is approximately 180 grams from my repeated tests. Now you need to add water to the jars, I recommend using 200mL. Make sure to take a spoon and press down on the kernels floating so that they also soak. Now place a filter disc in the retaining ring of the jar, screw it on, just snugg...not loose, but not so tight that you can't loosen it with possibly wet hands(Note: only use a filter disc, no lid is neccesary). Now take all your jars and put them in a cabinet or something incubating at room temperatures for about 24 hours(please don't go less then 20 hours-Thats when I've always ran into problems) The reason for this "pre-soaking" is to germinate any endospores which may be lurking in the rye, particullary the bacteria Bacillus.

Day 2
Prepare your pressure cooker to be used, now take how ever many jars your cooker will hold(Please not that Rye CANNOT be cooked on its side like cakes can, all jars must be upright) and place them in the pressure cooker, place lid on, and begin steaming, once steam is coming out of the valve for five minutes, place the pressure regulator on at a setting of 15 PSI. Once it begins to jitter cooker for 1 full hour. Now remove from heat. At this point I use a rapid cool method to get the next batch of jars in. I've heard this and that and the other about how this is dangerous, I've NEVER had any problem whatsoever with it except burning my hands from stupidity =P So what I do is first of all remove the pressure regulator, using an oven mit because its damn hot! Steam will begin pouring out of the cooker. Next I take a knife(one for cutting bread I think.. I'm not to handy around the house) and shove in the handle between the pressure lock and the pot, this causing the pressure lock to release pressure too. After steam stops pouring out I then tap the pressure cooker around with the knife until it releases. Now I remove the jars using an oven mit. Place the new jars on, add water if neccesary and repeat the proccess. While the jars are still REALLY FREAKING HOT, take a towel and fold it over a few times, and begin shaking the jars, you want to make sure the wetter kernels at the bottom mix with the drier kernels on the top.

Next I wait about 6-8 hours until the jars are cool to touch to innoculate. When innoculating I spray myself with lysol(Head Fingers Eyes Toes!) and then wash my hands with a waterless generic hand sanitizer picked up from my local grocery store. I DO NOT flame sterilize the needle I just rub it down with rubbing alcohol, then I loosen the lids to however many jars I'm gonna use on this one syringe(usually about 10-12 jars) and then I shake the syringe, wipe it with rubbing alcohol once more, and lift up the rings(the filter discs will stay in the rings) and squirt just about 1 cc of solution in the jar, and quickly screw the lid back on. after completing all these jars, I shake them vigorously, making sure to pound the kernels off the bottom (you want the spores to get mixed in with as many kernels as possible) Then incubate at 86F for Cubensis for about 5 days, then I go in, check for contams(yet to find one) and shake the jars vigorously breaking up all the kernels invidually(very difficult). You will notice it looks like all the mycelium dissapeared...this is not so. The kernels are still infected with that fungus you love, its just that the fluffy mycellia was broken up(actually innoculating more grain). Then I leave the jars alone until completely colonized(about 10 days for B+ and 8 days for Ecuadors...my experience)

Wheew... Well I hope that was of help to you guys.. Let me know what you think..any corrections possibly. I know this method has worked great for me and I've given detailed instructions to follow so that others can get it to work too...







Distributors of Mycological Products

http://www.MycoSupply.com




--------------------
Myco Supply - Distributors of Mycological Products
http://www.MycoSupply.com

The Premiere Source for Mushroom Growing Supplies.
Visit us online or call us toll free


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InvisibleBrownPastures
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Registered: 03/16/01
Posts: 968
Loc: here
Re: Old Rye Tek being brought back up... [Re: Lana]
    #350728 - 06/28/01 01:16 PM (15 years, 9 months ago)

kewl

"A Wise One like me should not charge for her services....The one who charges is a liar. The wise one is born to cure, not to do business with her knowledge."-Maria Sabina


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Anonymous

Re: Old Rye Tek being brought back up... [Re: Lana]
    #350731 - 06/28/01 01:19 PM (15 years, 9 months ago)

To wait 24 hours to germinate endospores, and then not flame sterilize your alchohol soaked instrument is insanity. And 0 contamination is a pipe dream.
My bad re read post. Syringe needle was used, was made under sterile conditions. No real neccessity to flame sterilize. 0 contamination is still pretty damn good. No matter what I do, still get a few contaminants.

Edited by Teonan on 06/28/01 08:05 PM.



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InvisibleRipper
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Registered: 01/20/00
Posts: 223
Re: Old Rye Tek being brought back up... [Re: Lana]
    #354441 - 07/14/01 12:53 AM (15 years, 9 months ago)

Hey Lana Sweety...

Well... nice to see my old tek brought up.. and 0% contams.. not a pipe dream.. sorry kid...

Those filter discs really kicked some ass in the past I must say...

A few new tid bits to throw in ... I'm not growing anymore, but I never posted finalized teks on everything.. so I'll throw in bits here and there...

Well... I found out it doesn't really matter either way as to whether or not you use the lids... only thing is with lids sometimes grain pops up through the little hole and gets stuck at the top, which sucks the wang.

Additionally.. I didn't really go into enough detail about how the the innoculation should be carried out... the A/C,VENT,HEATER err whatever needs to be turned off for 30 minutes prior... Then take a shower, put on fresh clothes... (preferably right out of a clothes drier) then make sure to clean all countertops with a disinfectant spray... (generic Lysol... Home Depot sells 1 gallon jugs for $8 that can be dilluted or used straight and works great with the smaller pesticide sprayers they use). I cannot stress enough to wear latex gloves... your hands really suck in terms of how much shit they throw off. Maybe wear a little painters mask(I never did this, but friends did suggest that this would prevent your breathe from coming into contact). Which brings me to my next point, whatever you do make sure that its like when firing a gun, practically hold your breathe or breath softly to prevent contams that way. Couldn't hurt to clean the kitchen hardcore. No dirty dishes obviously...

Hope that helps out some aspiring young growers...

if anyone needs to get in contact with me I can be reached @ rippergc@hotmail.com

Thanks.
Ripper




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InvisibleCondi1
old hand
Registered: 01/01/00
Posts: 502
Re: Old Rye Tek being brought back up... [Re: Lana]
    #354456 - 07/14/01 01:20 AM (15 years, 9 months ago)

Lana,
Had to at least say high. Not around much anymore, but noticed post. Glad to see you finally got around to playing.
As always, best of luck to you, and........,
Peace, as always. :)



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Anonymous

Re: Old Rye Tek being brought back up... [Re: Lana]
    #354468 - 07/14/01 01:41 AM (15 years, 9 months ago)

Rye was the first thing I got to work. I recomend it for anyone, especially beginners. Quart jars are nearly self containing, and you can case right in them. They are somewhat hard to pick from, but chop sticks work well to reach down into the jar. I was never that picky about the source of my rye (any local natural food store seemed to work) and did have remarkably low contamination rates now that I think back on my poor methodology in those days. I was always suprised so few people seemed to use rye...



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Offlinealuminum_can
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Registered: 05/18/01
Posts: 695
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Last seen: 14 years, 9 months
Re: Old Rye Tek being brought back up... [Re: Lana]
    #354727 - 07/14/01 04:25 PM (15 years, 9 months ago)

it would be easy to spawn a quart of rye with a few pounds of sterile manure right? i also heard that to innoculate (with filter disks) you just take of the lid band, stick youre syringe needle right through the disk and once youve innoculated you just put another one on top. i think that would be alot more sterile.

( . y . )


--------------------
the little kridders of nature; they dont know that thyre ugly!



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InvisibleLanaV
Head Banana
Female

Registered: 10/28/99
Posts: 3,086
Loc: www.MycoSupply.com
Re: Old Rye Tek being brought back up... [Re: Ripper]
    #354869 - 07/14/01 11:04 PM (15 years, 9 months ago)

Condi1, yes it has been awhile. You should come around more. Things here have changed a bit but things are still good.

Ripper.. Where you been sweeite? You don't write or drop by? Its ok, I know you're busy. If you have any of your old teks, send them to me and I'll post them if you like.

Ciao
Lana

Distributors of Mycological Products

http://www.MycoSupply.com




--------------------
Myco Supply - Distributors of Mycological Products
http://www.MycoSupply.com

The Premiere Source for Mushroom Growing Supplies.
Visit us online or call us toll free


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InvisibleCondi1
old hand
Registered: 01/01/00
Posts: 502
Re: Old Rye Tek being brought back up... [Re: Lana]
    #355320 - 07/15/01 06:47 PM (15 years, 9 months ago)

Heh Heh Heh,
Still hangin', but not able to check in much.
Best of wishes to you, as always. As you know, Bunyon stories always get me goin'! ;)
Heh Heh!
Peace



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Anonymous

Re: Old Rye Tek being brought back up... [Re: Ripper]
    #355411 - 07/15/01 10:00 PM (15 years, 9 months ago)

I understand the sterile technique pretty well, been micropropagated plants since 96. But no matter what the room looked like, how clean it was, how filtered all the air was, 0% contamination forever is not something I can imagine. Somewhere in the process a mistake is always made. You may go through several days without a problem, but eventually contamination hits. Anything under 10 % is exceptional. And that goes for commercial labs, and research labs. The only totally sterile places hold very deadly viruses. If you get 0% contaminants with any system, for as long a period of time as you suggest, you should change your occupation. Because you could make a fortune growing legal crops. No joke!!!!! Sometimes that extra few percentage points of less contamination can be worth a fortune. I can see how the system would work very well, the grain never is exposed to the air, the syringe is contaminant free, your injecting through a filter. But YOU NEVER HAD ONE ENDOSPORE HIDDING OUT SOMEWHERE iIN THE GRAIN. Not one contaminant!!!!!!



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InvisibleCondi1
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Registered: 01/01/00
Posts: 502
Re: Old Rye Tek being brought back up... [Re: Lana]
    #361582 - 07/26/01 10:09 PM (15 years, 8 months ago)

Heh Heh Lana,
Finally had the chance to check out your link. My, how some folks working knowledge can progress over time! Guess I'll have to be asking you for advice in the future! ;)
Peace, as always.



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InvisiblePerma
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Registered: 04/24/00
Posts: 776
Loc: Ontario, Canada
Re: Old Rye Tek being brought back up... [Re: Lana]
    #365049 - 08/01/01 10:58 PM (15 years, 8 months ago)

Hey that's a great tek Ripper, thanks Lana for bringing it back up. I printed it and added it to my binder with all my other teks. I'm definately tempted to try Rye, the way Ripper describe his tek makes it sound easy and promisable. I don't use enough grain in my growing, it's time I move on to the real shit.



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OfflineAnnoA
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Re: Old Rye Tek being brought back up... [Re: Lana]
    #370783 - 08/12/01 09:38 PM (15 years, 8 months ago)

Soaking the rye for 24 hours is an overkill, especially in warm climate. It begins to germinate and spoils your efforts.
I newer soak the rye and have no problems. I?m aware though that some rye brands carry a greater amount of endospores than others.
Soaking for 12 hours, or placing the jars into fridge during the (longer) soaking should fix the germination problem.

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Amazon Shop for: ½ Pint Jars, HEPA Filter, Laminar Flow Hood, Pressure Cooker, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Substrates

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