fontinalis666 said:

What sort of lids are you using? The grains look awfuly close to touching the lid, or maybe they are touching it, I'd be worried about that as a possible contam vector personally.

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Fiet said:

My Quart Jars with Jack Frost LC after 20 Day's for both Jars on the left

On the Right they 17 Day's old

All 4 Jars do Shacke 2 Times after they recover from a Big Shake couple Days ago

How it looks too You?

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Jars look good! No need to do more than a shake, some do 2. more doesn't offer any benefit, in fact, too many shakes can stress the mycelium out as it needs to recover each time

FITMK said:

fontinalis666 said:

What sort of lids are you using? The grains look awfuly close to touching the lid, or maybe they are touching it, I'd be worried about that as a possible contam vector personally.

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They're the standard Ball two-piece metal lids, nail punched 1/4ish hole, poly stuffed. Since inoculating these particular jars I've upgraded to plastic ones with SFDs, which is where those cultures in the plate pic will end up.

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Lo-Z said:

I havent dont an agar roof yet. So there's really no way to prevent post cabin condensation? Im assuming its okay for the myc?

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Well mycelium grows fine in LC so the water won't be a problem, but it isn't ideal giving the bacteria a medium to move fast in. On the flipside it might make the mycelium speed up trying to get away from it.

I like the island tek Agar Wedge posted the other day and I'll try to pour some next time I do agar. For the most stubborn bacteria a water agar cabin seems to work best, so maybe pouring some water agar islands will be a good option as long as the island plate always sits flat

DV8DUG said:

Ooo...
Water agar island...

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I was just thinking about your double condiment cups cleanup, would it be possible to do something similar there, like having one of the cups being water agar

Agar Wedge said:

That's a really good point, using water agar for the Island will help further cleaning. Just made me realize I didn't mention the laying flat part. Good looking out! Gonna go update the OP

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Haha, yeah I can be clumsy and is probably some of the reason I like the cabin over trench, trap door, sandwich and island

bloodssonnew said:

Or antibacterial island agar o.o double-down on that bad bacc

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bloodssonnew said:

Or antibacterial island agar o.o double-down on that bad bacc

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Good point, I can't get that here though

Tri said:

Quick question my smart friends




This germ plate grew out to this point and has stalled without reaching the edges, I know with regular transfer plates that’s a bad sign but does the same apply with germination plates? Both possibilities make sense to me so QQ time

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Agar Wedge said:

Fiet said:

My Quart Jars with Jack Frost LC after 20 Day's for both Jars on the left

On the Right they 17 Day's old

All 4 Jars do Shacke 2 Times after they recover from a Big Shake couple Days ago

How it looks too You?

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Jars look good! No need to do more than a shake, some do 2. more doesn't offer any benefit, in fact, too many shakes can stress the mycelium out as it needs to recover each time

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Tweeq said:

Germ plates usually have many contams riding along. And also the different myc colonies end up fighting each other.

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bloodssonnew said:

I've noticed with germ plates the colonies can kinda sense the space and nutrients a plate has n sometimes stop growing n go into a kinda preservation mode when transfered to a new plate had em takeoff and continue growing then fill out the plate

Could be like stated above there's hitchhiking contams as that's pretty common from spore

And on another note monos tend to not fully colonize a plate though can be transfered and will grow out some more

Just saying what I've noticed from little experience.

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Agar Wedge said:

That's a really good point, using water agar for the Island will help further cleaning. Just made me realize I didn't mention the laying flat part. Good looking out! Gonna go update the OP

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Mackload said:

recharge desiccant packs in dehydrator y/n

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certainlysarcastic said:

Hi all. Does anyone know the cause of this? It kinda looks like the cap is breaking apart. It’s only affected a couple pins so far but I grabbed them out in case it’s more serious. Bacteria? Overwatering? Thanks

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Looks like rosecomb mutation, have you been spraying your fruits with iso by accident

It also looks like you're growing Toque genetics, if that's the case many of them are highly unstable and will throw mutants often, should be fine either way

callmehippie said:

Here's a question I've never considered.

Let's say you've got some jars 100% colonized and consolidated. you break and Shake before going to bulk. So that you can watch recovery and make sure nothing's hiding..

Few days later.. you've got great recovery.

Is it now okay to go forward with going to bulk ? Or are you supposed to wait for it to fully colonize again.

I feel like I always just went for it at the sight of good recovery.  and never waited for full colonization after that final b&s.

But I've been away for about 9 months and can't remember.


Ps. Looking for people that do a final break and Shake to check recovery before going to bulk to answer please.

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