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Re: Official Copelandia/Panaeolus Thread [Re: Nichrome]
    #29482624 -

Nichrome said:
conz0r said:
Foreighn2891 said:
returning back to conversation about dark spot on agar, this one are clean, everyone sample of this specie gives same color which changes depending on the age, later it will turn to white. Similar situation with Bisporus and Cinctulus, all three are giving very specific looking mycelium on agar


What dya' think it is? Metabolites?


melanin

Honestly, I got no clue, maybe certain specie’s natural appearance? On my hands 3 different and all has specific appearance, the one on photo is a clone of ingeli, other two Bisporus and cinctulus this two gives powdery mycelium on agar, but all of them do successful growth, ingeli so far not fully successful growth but that not about mycelium colour but about environment. Here’s clone of Bisporus on picture

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Re: Official Copelandia/Panaeolus Thread [Re: BlimeyGrimey]
    #29482629 -

BlimeyGrimey said:
tree frog said:
Does anyone know if any common cyan strains going around are both sequenced and on iNat?  I guess Marcos Island would be an option :lol:


Panaeolus sp. 'Weza' is on iNat and MO with sequences.

https://www.inaturalist.org/observations/277412413

https://mushroomobserver.org/observations/516883


Anyone know where to send Pan and Gym species for ITS sequencing? OMDL isn't accepting submissions last I heard, and Mycota doesn't have an open-submission policy at the moment.


Appreciate that fam!  Guess I need to run faht's Weza clone again :lol:

I know there's a lab in CO that does sequencing for a small fee (I can find more information on that).  But I generally talk to Scott directly (from OMDL) for Pans to see if it's something he's interested in looking at.  That's how we're getting Komodo Island done, and who did the sequencing for Mystery SA and Bisporus for us (my samples).

You can DM me if you'd like me to faciliate getting in contact with him directly.  But his email is likely on the web and he has a patreon too.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29482631 -

I’m sure Alan knows every lab testing, worth asking him.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Official Copelandia/Panaeolus Thread [Re: BlimeyGrimey]
    #29482638 -

It looks like Weza is the same species as Mystery SA.  SAO2.  I will be adding my observations of that in Advanced mycology and also regrowing fahts clone to scope it.

But it looks like it's the same one I've been growing and sharing as mystery sa/beach pan.  Tea tree is also likely this species.  But I haven't scoped it yet only observed the fruit bodies and mycelium.

See below


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/13/26 05:56 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29482679 -

Is this the same Weza? https://www.shroomery.org/forums/showflat.php/Number/26564382

If it is they were skeptical of it being cyan from the beginning. Pics are gone though.


--------------------
what i've been growing

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Re: Official Copelandia/Panaeolus Thread [Re: arlo.arlo] * 3
    #29482711 -

This is the one I believe is going around as Mystery SA and that Scott sequenced.

https://www.shroomery.org/forums/showflat.php/Number/26382522#26382522

And these I've been trying to fruit for half a year.  The culture is clamped but goes dormant on my typical casing recipe.  Tea tree is also in this thread.

https://www.shroomery.org/forums/showflat.php/Number/27558264

Weza has been crossed with a cyan.  And I assumed it was a cyan so I honestly haven't observed it closely.  Mystery SA can express very much like a cyan though.  And maybe it's more open to crossing then chlorocystis and bisporus (all three are two spored).



Mystery SA/SAO2 is pretty easy to ID with a scope because the spores are huge and distinctly shaped.  Though I don't believe I have a weza print right now to confirm (I will look though).



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 3
    #29482726 -

Thanks to faht I have a Weza print in my stash. Looks like a cyan.



Tea tree looks like SAO2



And here's a couple of SAO2/Mystery SA shots for reference.




Probably my favorite spores in the subgenus next to the very rare heart shaped ones chlorocystis throws.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: Mackload] * 1
    #29482956 -

Mackload said:
3-4 days?


Got it, with pans I don't like to see any wet grains, but if it was prepped wet and had some burst grains it might still be worth spawning.

I would give them another week and see how they look. The one to the left I would try spawning as long as it doesn't smell like shit when you open the jar :lol: But yeah give them a week.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 1
    #29483008 -

Kloakk said:
Mackload said:
3-4 days?


Got it, with pans I don't like to see any wet grains, but if it was prepped wet and had some burst grains it might still be worth spawning.

I would give them another week and see how they look. The one to the left I would try spawning as long as it doesn't smell like shit when you open the jar :lol: But yeah give them a week.


:rockman:

Thanks, bro!


--------------------
Learn it, know it, live it


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Re: Official Copelandia/Panaeolus Thread [Re: Mackload] * 2
    #29483431 -

Been a minute since I've gone with pans...here are jars from Jan 28:

My TTBVI is doing great:


My Bunnell is getting there (obvious growth was a few days behind TTBVI) but is wispy.


Other jar of Bunnell and single sample of Bisporus are showing 0 growth. 4x cube jars are showing excellent growth on same batch of grain.

And on my jars innoc on Feb 6 - out of 12 jars (let's say 6 on the same oats and 6 on new batch of rye berry), only one (Bunnell) is showing growth, and very little at that. I can see in 2 or 3 jars there are agar chips on the side that haven't recovered at all.

So, I'm just refreshing my expectations on if that's normal or if I should go ahead and inoculate some new jars and assume this current 2/6 batch has failed. Obviously I wouldn't chuck them just yet and would wait another week, but in an effort for efficiency. I'm still narrowing down 'producer' genetics overall.

*For reference, I use flowhood and have VERY little contam rates on my agar, and the cubes on the same grain is going great so I doubt the grain is compromised.

Any thoughts on the two no shows from 1/28 or the no growth on almost anything from 2/6?


--------------------
My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

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Re: Official Copelandia/Panaeolus Thread [Re: conz0r]
    #29483436 -

conz0r said:


I have to use it a little more sideways than intended but it took 20 seconds to zero in the distance. Fucking pwnage for $15.

Right let's do :agar:


Hehe my go to is the stuff I use for electronics. Dental loupes for 3.5x mag at a reasonable distance. Cheap AF from Aliexpress.


--------------------
My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente]
    #29483473 -

If one were to adjust a BRF PF tek for pans that have very thick mycelial growth and have trouble fruiting, should I be possibly using less BRF? Maybe 0.5 jar BRF/ 2.5 jar verm / 0.75-0.8 jar water?

Should I cut back on the water at all?

Would adding a very tiny amount of soil, maybe a couple table spoons, be problematic?

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon] * 3
    #29483497 -

possibly maybe
r u a bjork fan?
the only thing you should cut back on is wasting time and the one thing you should definitely do is follow a tek and finally get real.
macmerdins pan cakes, have a look if you want


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29483526 -

tree frog said:
Probably my favorite spores in the subgenus next to the very rare heart shaped ones chlorocystis throws.


I'm giving myself motion sickness trying to find a heart shaped spore


--------------------
what i've been growing

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Re: Official Copelandia/Panaeolus Thread [Re: chris77]
    #29483529 -

Well since I'm possibly working with a new species I was looking at what adjustments seem relatively moderate and sensible to make with supplies I have available. Just wasn't sure if maybe I needed to dial back on the jar nutrients or moisture some. I have already been following the standard PF tek for BRF, but when it comes to Pans this isn't as standard as for cubes. There are already several adjustments written about.

From my experience, I don't think I would have much success with innoculating in the center with a nutrient core and casing around the perimeter in my jars like the one exotic method suggests.

If you read through all of macmerdins pan cakes thread you will see they try to grow another Panaeolus species as well but he failed to do so.

The goal is to keep things tidy and contained in jars, because of what I have observed from my grows. Burying every cake in something is a huge mess and extra cost.

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29483540 -

Idk if we have seen you post your available gear...but what everyone will recommend is doing the known basics.
Multispore >> Agar >> T1-T2 >> some transfer method to grain >> standard bulk recipe.

If tissue, then Tissue > water agar >> T1-T2 >> some transfer method to grain >> standard bulk recipe.

I didn't read the initial link but here's another.

But I'll be as blunt as everyone else. You need to:
1) Get some actual grows start to finish done to build confidence. (If they weren't satisfactory then get one you deem such, only that counts).
2) Just literally do something. You've debated alot of theory. Maybe someone's done exactly what you want, but you're saying you potentially "have a new species" therefore the ownness is on YOU to figure it out. That's the fun. if you want blank yields go known species and tried and true. If you're experimenting, then dedicate to experimenting. And again, usually the best place to start is the default everyone does.



No one can guarantee your grow, or get you to 100% confidence rating. Jump in, commit some BRF or oats. They are CHEAP AF. The only lost resource is time. Which again, if you 'need yiedl now' go with KNOWN stuff (species, easier grows, etc).

Edited by Kunta Kente (02/13/26 05:43 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente]
    #29483559 -

Maybe TF will chime in if she sees this with a suggestion on if I should curb back BRF slightly or not, and how low in nutrients would be sensible for a jar grow with some of the Panaeolus species she's worked with.

I only have 4 jars open, so I guess I'll try 2 regular ratios, and 2 with the adjustment I stated, using some cloned material from a fruit I was able to get.

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Re: Official Copelandia/Panaeolus Thread [Re: arlo.arlo] * 1
    #29483585 -

arlo.arlo said:
tree frog said:
Probably my favorite spores in the subgenus next to the very rare heart shaped ones chlorocystis throws.


I'm giving myself motion sickness trying to find a heart shaped spore


Right lol

I've observed four.  And I wasn't looking for them, I noticed one while doing other observations and then remembered that they were in the keys.  I have photos of three of those (somewhere) but this one is centered.  So this is what they look like.



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29483586 -

Maybe I will try 2 of the exotic tek method. I have already tested some middle innoculations and it is easier to fail this way which was why I am scared. Hoping I can get a clone to perform.

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29483589 -

tultedspoon said:
Maybe TF will chime in if she sees this with a suggestion on if I should curb back BRF slightly or not, and how low in nutrients would be sensible for a jar grow with some of the Panaeolus species she's worked with.

I only have 4 jars open, so I guess I'll try 2 regular ratios, and 2 with the adjustment I stated, using some cloned material from a fruit I was able to get.


It's my suspicion that most of the pans we grow in this thread will fruit off of regular BRF, since they fruit of cased popcorn.  But we're not sure what species you're growing and what its needs are.

But like Chris said, if you want to modify it the first place I would look is mac's thread.  I cribed a lot from him when I first started and those approaches are still absolutely viable.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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