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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 5
    #29482283 -

Nigthcap.  It's from Australia I believe.  Pins easily on plates too.

I've only worked with crosses of it because my landrace aborted.  But I have it going again.

Nightcap * Eungai (also Australia that pins easily on plates)


Nightcap * Oregon (the photo doesn't do the color on these justice, creamy orange caps and creamy stipes.  I'm running it again as I didn't get prints the first round because I wasn't impressed with the cap size...  but I kept dreaming of the color :lol:)


edit:  better picture



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/12/26 11:46 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: conz0r] * 1
    #29482290 -



I have to use it a little more sideways than intended but it took 20 seconds to zero in the distance. Fucking pwnage for $15.

Right let's do :agar:


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: conz0r] * 1
    #29482295 -



Shook twice I think,  maybe overshook. Prepped grain too wet. PHV. One jar looks ok, if wet, the other hasn't recovered. Is that a toss?


--------------------
Learn it, know it, live it


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Re: Official Copelandia/Panaeolus Thread [Re: conz0r]
    #29482309 -

So I'm not gonna waste space with stuff I know is good and j can transfer easily. But this one's my big worry, it's B-bar. Check this shit out, it's been settling in for a week now... Bacterial AF.



What do I do about that? Swipe the top off with my noc loop and transfer it?


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: conz0r] * 1
    #29482323 -

On mobile doing pics isn't easy with this piece of shit. So!



That's nec-D the other one I'm worried about. I dunno where I should be taking from this message honestly.



That's eungai, feel pretty comfortable with that one.

*** They're all T+0 and done my MD - judge his work yourself.



That's ttbvi and popcorn you can see. Again they shouldn't be too hard to figure out lol.

*** They're all T+0 and all done by MD - you can judge his work for yourself.


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: conz0r] * 1
    #29482326 -






In order - 1Tampanensis 2Byron bay 3komodo X ttbvi 4ingeli

....they're all piss poor mostly.


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: conz0r] * 3
    #29482337 -

My little workspace...



This, a spliff and some music while my cat sits on a chair and watches



--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: conz0r] * 1
    #29482343 -

You know what, here's one I cleaned up from a plate he gave me free on the first order.



Not that I've got anything to prove but ya know...

We're not moving till we know what we're doin'



--------------------
Be lucky, may light walk with you.





Edited by conz0r (02/12/26 02:34 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29482402 -

tree frog said:
Nigthcap.  It's from Australia I believe.  Pins easily on plates too.

I've only worked with crosses of it because my landrace aborted.  But I have it going again.

Nightcap * Eungai (also Australia that pins easily on plates)


Nightcap * Oregon (the photo doesn't do the color on these justice, creamy orange caps and creamy stipes.  I'm running it again as I didn't get prints the first round because I wasn't impressed with the cap size...  but I kept dreaming of the color :lol:)


edit:  better picture




Nightcap was fun. Bigger sparser fruit. Nice change of pace when harvesting compared to something like bvi.


--------------------
what i've been growing

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Re: Official Copelandia/Panaeolus Thread [Re: arlo.arlo] * 2
    #29482424 -

Started a nerd thread with microscopy, mycelium, and fruit body photos.

Bisporus and Pan India are complete.  Probably start on beach pan/mystery sa next.

We'll be getting Komodo Island Cambo sequenced soon, and I'm running that out again to fill out that species.  My chloro specimen needs to be resequenced, came back two base pairs from cyans, but I sent the lab not-trop and Oregon too and I imagine a sample got mixed up.

Does anyone know if any common cyan strains going around are both sequenced and on iNat?  I guess Marcos Island would be an option :lol:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29482429 -

Tree-frog could I please get your advice on this one?;

It's B-bar and there's no smell, 7 days it's been recovering after an 8 day journey. Edit: I haven't sniffed it, but I can't smell anything coming off the plate and I'm a dog, also no growth.


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: conz0r] * 1
    #29482436 -

You know what I'm sorry I know what to do... I dip my noc loop in a little of the side, swipe it across divets in a new plate... All the chatter I read between you guys fills in somehow. Then leave it since there is no contam, as genesis has just said.

But my thinking is on the right lines im sure if it. Sorry ppl I don't mean to spam the thread xXxX


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: Mackload] * 1
    #29482439 -

Mackload said:


Shook twice I think,  maybe overshook. Prepped grain too wet. PHV. One jar looks ok, if wet, the other hasn't recovered. Is that a toss?


How long since you shook them?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: conz0r] * 4
    #29482453 -

Awesome microscopy posts treefrog.  Will be using them for reference.  I need to update my pan library with some of these rarer varieties.

With phase contrast lenses, found it hard to view the sterigma so I’m switching to regular lenses next with koh staining. 

With the phase lenses I think I can see the nucleus in younger spores.  I think I’m looking at some spores with two, nuclei.

400x attempted  ttbvi x pan bisporus cross.



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Re: Official Copelandia/Panaeolus Thread [Re: conz0r]
    #29482477 -

conz0r said:
Tree-frog could I please get your advice on this one?;

It's B-bar and there's no smell, 7 days it's been recovering after an 8 day journey. Edit: I haven't sniffed it, but I can't smell anything coming off the plate and I'm a dog, also no growth.


Sorry I missed this. Cabin sequester is my advice.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: trippleblack]
    #29482485 -

Nuceli are very small, so what you're observing is likely the membrane around them.

But you're right, in the sense that those spores are from two spored basidia.

That said, cyans do occasionally produce two spored basidia.  Sometimes this is even the dominant expression in them, such as in the case of the Marcos Island cyan that was misidentified as bisporus.

https://www.inaturalist.org/observations/154428381


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29482553 -

tree frog said:
conz0r said:
Tree-frog could I please get your advice on this one?;

It's B-bar and there's no smell, 7 days it's been recovering after an 8 day journey. Edit: I haven't sniffed it, but I can't smell anything coming off the plate and I'm a dog, also no growth.


Sorry I missed this. Cabin sequester is my advice.


Cheers ma dears, yeah I'm just digging into callmehippie's piece. Have a good night party people. Eye strain has frazzled my nut :migraine:

Super duper happy it's all ok 👍

Night night guys Nd gals xXxX


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk]
    #29482568 -

Kloakk said:
Mackload said:


Shook twice I think,  maybe overshook. Prepped grain too wet. PHV. One jar looks ok, if wet, the other hasn't recovered. Is that a toss?


How long since you shook them?


3-4 days?


--------------------
Learn it, know it, live it


Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29482611 -

tree frog said:
Does anyone know if any common cyan strains going around are both sequenced and on iNat?  I guess Marcos Island would be an option :lol:


Panaeolus sp. 'Weza' is on iNat and MO with sequences.

https://www.inaturalist.org/observations/277412413

https://mushroomobserver.org/observations/516883


Anyone know where to send Pan and Gym species for ITS sequencing? OMDL isn't accepting submissions last I heard, and Mycota doesn't have an open-submission policy at the moment.

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Re: Official Copelandia/Panaeolus Thread [Re: conz0r]
    #29482615 -

conz0r said:
Foreighn2891 said:
returning back to conversation about dark spot on agar, this one are clean, everyone sample of this specie gives same color which changes depending on the age, later it will turn to white. Similar situation with Bisporus and Cinctulus, all three are giving very specific looking mycelium on agar


What dya' think it is? Metabolites?


melanin


--------------------
Question everything, it's very responsive.

Culture Storage

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds High THC Strains

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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