conz0r said:

Could I ask anybody that uses a Martha tent, have you noticed any benefits to giving each tray space from one a other? Or are they just as happy to be wall to wall in there? I'm just wondering if crowding will affect O2 levels and maybe hinder evaporation.

Side note - I'd love to see some kinda Martha tent setup being fed O2 through a hose like with humidity, see if gigantism could potentially be triggered or even maybe just larger fruits?

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conz0r said:

Ha!
I shall do no such thing you have my solemn word
Just curious was all, the O2 was in reference to producing larger fruits not as a way to eliminate CO2 build up I promise. Interesting premise no? Adding O2 to see if fruits increase in size?

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PuffM4gicDr4gon said:

conz0r said:

Ha!
I shall do no such thing you have my solemn word
Just curious was all, the O2 was in reference to producing larger fruits not as a way to eliminate CO2 build up I promise. Interesting premise no? Adding O2 to see if fruits increase in size?

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It's about as interesting as tapdancing on molotov cocktails while your legs are burning

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Quote:

Post casing pre-pinning:
Relative humidity: 90+ %
Substrate temperature: 79-84 degrees f.
CO2: 10,000 ppm or higher.
Fresh air exchanges: 0 per hour
Light: Incubation in total darkness.
Primordia formation:
Relative humidity: 95+ %
Air temperature: 75-80 degrees f.
CO2: 5,000 ppm or lower.
Fresh air exchanges: 2 per hour
Light: Diffuse natural or fluorescent grow-lights.
Cropping:
Relative humidity: 85-92 %
Air temperature: 75-84 degrees f.
CO2: 5,000 ppm or lower.
Fresh air exchanges: 2 per hour
Harvest stage: When caps are covex.
Flushing interval: 5-7 days.
Light: Diffuse natural or fluorescent grow-lights.

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tree frog said:

I was running one over the winter.  My fruiting chamber never got close to 5000ppm, even when full of projects.

And it's just 25 gallons, much smaller than a tent.

I didn't bother installing it when I rebuilt my tubs recently.

I think the fresh air exchanges are important.  Helps promote evaporation.  But once or twice an hour seems like all we need.  I think faht has a fan blowing on his Ziplocks once an hour for example.

My current setup just runs the fogger and a couple of computer fans for two minutes every half hour.

These are late flush, but you can see they look happy.

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I also got approximately same set up as yours, just slightly different fan design of “turbo” cooler from 3d printer and built a small receiver to help increase an air flow true a filter, filter itself right now became needless but it is what it is 😁 to lazy to modify it again. Haven’t got it yet but working on better understanding, seems that fogger not really needed before pins popped up and not sure how the air flow affecting on fruits itself, with pans no problems but other species looks like need still air tho, just guess.

tree frog said:

I think that's a lot bigger than the space I am using.

When I was using a tent I had an HVAC tube with a 4" exhaust fan on it.  So sucking out instead of blowing.

But I use four computer 3" fans and the speed is adjustable. Right now they are on one.

One exhaust on each fruiting chamber.  And two on the climate chamber blowing in.  But the humidifier also pushes quite a bit of air in.

They all run together on a timer.  Not that I really trust the humidity sensor at such high humidity but it's always at 100 basically.  They tolerate high humidity better at cooler temps (72-74 at night and 74-76 day).

My setup looks like this.






And there's like six seed mats. Two on top of the fruiting chambers. And four under the climate chamber.

I know that doesn't really answer your question. But you need to calculate the square footage of your space, and then compare that to the CFM of the fan.

Either that or just get adjustable fan so you can dial it in using the fruit morphology.

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I like what you've done there, it's a solid setup, seems bulletproof. Yeah I'll grab a CO2 sensor and  tweak it as I go but fit 6 basic fans, one high one low for each 3 walls sucking in with the door perforated somehow, adjustable fans aren't too pricey on eBay either.

It answered the situation in my head clearly, thanks again. Oh and in transit are seeds for fly eating plants 😼 and cat is on gnat patrol, they're the fucking devil!

trippleblack said:

Any microscopy experts in here know how to prepare a sample to view basidium and sterigma?

Almost thought I found a 1 spore basidia but this gotta be cystidia right? Should I try to slice off gills and lay flat?  I did a crush mount by slicing cap in half. 

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Not an expert but getting better.  I put together this for a friend recently, should probably put a write up together for patreon and the boards.  And yes, those are cystidia (also called metuloids, the thick walled wine bottle looking ones anyway).

Broke the cap open.  (this is a bsrs * bbar cross)


Pulled a gill out with some tweezers.


Put it on the slide.


Cut most of it away so I had a very small fragment of the gill edge (where the spores are rather than the towards the top of the cap).


Discarded the rest of the fragment and moved the small piece to the slide.


Added a bit of koh (a fresh gill you can skip this but it helps with tissue bruising.  With a dry specimen use koh or water)


Used the tweezers to push the sample down into the koh.


Added a cover slip and pressed firmly to remove air bubbles and flatten the sample (don't smear and don't press so hard you stress fracture the slip).  Also note the gloves, koh is a base though it's very diluted.  And finger prints aren't helpful.


All prepped.


Unaltered images.



Altered image to point out what you're looking for.


And I find them mostly along the edges of the sample.  (This one is just bbar for all the bbar fans )


This one is bisporus.  Can you spot the two spored basidia?  There's several in that image with spores still attached. 



And it's much easier to confirm with attached spores, as you can see from this second altered image of a four spored basidia without the spores.



I don't use dye by the way.  Though I imagine it would help.  I just play with my condensor so that the clear cells are more visible.