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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29472488 -

That's what I was thinking. If the end goal is to be able to have moist peat that doesn't mold then I should be able to trial and error it a bit.

I wont have spawn for a week or two anyways, so waiting and seeing isn’t a big deal.


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 2
    #29472517 -

fahtster said:
I usually look for larger (with pans) fruits in a cluster that look healthy with good morphology.

This is a clone.. flush 2ish



For your ziplock technique did you have to do some initial selection to find something that did well? Then clone from there?


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Cultivating Panaeolus cyanescens


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Re: Official Copelandia/Panaeolus Thread [Re: Asura] * 6
    #29472834 -

Tub from previous page, harvested today.

Should hopefully be a bvi x bisporus, doing the microscopy now and almost ready to spawn multiple other variations.that look different. 


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Re: Official Copelandia/Panaeolus Thread [Re: trippleblack] * 1
    #29472851 -

Well I decided it's time to try some more Pan TTBVI. Put down some Semps as well but I may have to pull out another print for them, as none of them has germinated yet.



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Re: Official Copelandia/Panaeolus Thread [Re: RockinRobot] * 1
    #29473461 -

:carlton: Heya party people... Just getting this in my threads list. Just posted a new thread for a bloody pan question like a nooblet :picard: pans are what I'm focusing on, I really look forward to kickin' it here. The work I've seen in this thread was my inspiration.


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Be lucky, may light walk with you.





Edited by conz0r (02/04/26 10:32 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente]
    #29473465 -

Kunta Kente said:
el boso said:
Happy I got fruit, not happy with the amount or size..this is pan bispo Washington and it was a transfer from a Ms plate.
If I want tall fat canopy fruits what do.i do next?




Ok....sorry for anyone more educated, and for those who have heard it.

Genetics:

Spore = 1n
Mycellium aka hyphae lets say are always 1n+1n.

You take a chunk from a MS plate and now you have a grain jar (and then tub) full of X (1n+1n) "individual colonies" competing for nutrients, water, and space. They did that all in the grain, and the war continued in the tub.

Now you have fruiting bodies. Each is 1n+1n and no longer X(1n+1n). You have effectively isolated genetics, similar to going MS (To) > T1 > T2 etc.


BUT

Scenario A) if you go MS > > T2, you are fairly confident you are growing a single genome 1n+1n in your jar, and fruits. That is IDEAL fruiting conditions. If it does well then, it should in the future. Now you reasonably KNOW you will consistently get whatever lottery you grow then. Be it large fruits or many fruits, or if they cluster or don't.

Scenario B) You clone from that big boy fruit in the MS tub. Yes it is likely a genetic isolate. Yes it on agar will likely always be the same 1n+1n you cloned from. BUT, you have no idea if grow #2 will end up with: same sized fruits, many fruits, less fruits, etc....because that isn't what you selected for. You selected for one of the most aggressive genomes that did well in that jar, in that tub, vs all of those other genomes. You didn't really select for "the biggest one" or "the one that fruited so next time it will fruit a ton" despite trying to.


====whereas if you went scenario A and got lucky to get one you deem "a good grow" you can be quite certain you will always get that good grow. Because that genome always saw the same jar and tub conditions every time.



Sorry if that's unneeded.


tldr; Take it, quarter the stipe, sterilly clone it to agar, try grow 2.


:nice:

That blew my mind. Just got me an education, thank you!


--------------------
Be lucky, may light walk with you.





Edited by conz0r (02/04/26 10:42 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente] * 1
    #29473519 -

Small correction, Panaeolus bisporus is psuedo-homothallic, so n+n even from a single spore.  Not all of these will be compatible mating types, so often they will still cross with another strain (the bisporus strain on the plate, not with like cyans).

And this part is my opinion, not proof.  Though it's an informed opinion from reading David Moore's textbook.  But I believe that by the time we spawn the war is over.  The genetics have already sorted themselves out through the parasexual cycle, and the weaker strains died out.  I think this often can happen with pans by t2.  Especially in the two spored species.  And part of the reason I believe this is because of the break and shake.  The substrate acts as a colony, not a bunch of small colonies spread through a large substrate.

So, and again just my opinion, the benefit of cloning isn't in narrowing down the strains.  But in epigenetic markers.  The cloned strain has learned what conditions to expect.  And an isolate from that same plate prior to cloning has not.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/04/26 12:20 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29473533 -

Certainly color of mycelium on agar is different then syanescens. first impression was that is kind of mold or parasite with mycelium together but definitely not healthy material 👍🏻

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29473551 -

There's some bispo mycelium photos at the end of this post.  All on 0.5% lme agar.

https://www.shroomery.org/forums/showflat.php/Number/29180779


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29473561 -

:chat: Oh my god it's tree-frog!

Good to see you here!

Seeing the process play out, as competition thins the "herd" it's also logical that only the strongest would remain to consolidate - n1 citing break and shake.

Awesome observations - again!


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: conz0r]
    #29473574 -




I'm misting when surface has no sparkle although it'll get a break till morning at least. Now I dunno if it's reaching up saying more air! More water! Or hey bro!...

Can I get a whoop whoop?


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: conz0r]
    #29473584 -

conz0r said:



I'm misting when surface has no sparkle although it'll get a break till morning at least. Now I dunno if it's reaching up saying more air! More water! Or hey bro!...

Can I get a whoop whoop?


„Pinning’s begun? Touch nothing, son.“
:wink:

iow LITFA

congrats!


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29473644 -

serms that different or I have both hands left 😁

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper] * 1
    #29473670 -

Looks bacterial.  If you want to save it I would run it through a cabin (or two).

Or go back to spore if you have them.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29473700 -

that’s how looks like mycelium of Bisporus I got, and I had a couple grows from that plate. Looks wired but once it gets on a poo it’s turning white.

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29473728 -

Foreighn2891 said:
that’s how looks like mycelium of Bisporus I got, and I had a couple grows from that plate. Looks wired but once it gets on a poo it’s turning white.


seriously? i‘ve had several plates of bisporus - never looked anything like that
:justno:


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Copelandia/Panaeolus Thread [Re: chris77]
    #29473736 -

Every time same picture on LMA agar, (var. Washington), regardless if that’s from spores or clone. I guess that culture came from Bra . And same picture with cinctulus but colour is creamy

Edited by Noah Keeper (02/04/26 05:08 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29473737 -

It's easy to get bacteria into a culture on accident when reviving it, depending on how it was handled by both you and Bra.  Even when Faht sends me stuff I run his grains through a cabin, because it's easy for me to cross contaminate fishing them out of the packaging.  I'm sure Faht is careful, and I'm careful, but a cabin gives me peace of mind.

And it's possible the bisporus doesn't care.  Not all bacteria is harmful to fungi.  The main thing is the grains colonize so mold doesn't get to the carbon, and get a foot hold in your substrate.

It also could be old, it does turn darker as it ages.  I don't know how you stored it or what your nutrient percents are.  I just know that if I have a plate that's not fully colonized and yellowing it tends to not do well.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/04/26 05:13 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29473747 -

Agar is nutritious, unfortunately everything measures on “fish eye”😅 roughly 3 gr of malt extract per 100ml of water plus agar itself. Everything made under laminar hood. Thanks for info about bacteria, interesting theory, that’s exactly what we get in pasteurized soil, thermofillic bacteria and helpful mycelium from nature alive for assistance.

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29474302 -

Could I ask anybody that uses a Martha tent, have you noticed any benefits to giving each tray space from one a other? Or are they just as happy to be wall to wall in there? I'm just wondering if crowding will affect O2 levels and maybe hinder evaporation.

Side note - I'd love to see some kinda Martha tent setup being fed O2 through a hose like with humidity, see if gigantism could potentially be triggered or even maybe just larger fruits? :discorex:


--------------------
Be lucky, may light walk with you.





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