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Sulfurshelfsean
Defender of Cubes

Registered: 07/29/10
Posts: 9,273
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: cronicr]

1
#29353441 - 09/23/25 08:16 PM (1 year, 21 days ago)

Does this look okay? I'm trying to clean up some cultures I have that always seem to do good up until I fruit and get green mold out the ass. I took a couple pieces of the very edge and transferred. I have a feeling the fuzzy growth is a contam.

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AZ Starman2112
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Registered: 12/11/24
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Sulfurshelfsean]

10
#29354071 - 09/24/25 11:28 AM (1 year, 20 days ago)

Beautiful Yeti T1 6 1/2 days old.

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Geezed
Blessed

Registered: 04/15/23
Posts: 74
Loc: Lost Lands
Last seen: 5 days, 7 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]
#29354516 - 09/24/25 07:08 PM (1 year, 20 days ago)

Geezed said:

Will these explode from thermal shock if I take them from PC @ 150 f and put them in a cooler temp at say consistent 120 f?

Just nervous after the quart jar 9ish years ago of LC exploded on me. Do to me not paying attention and went to go wash my hands in the sink next to my LC got hit with cold water. Lesson learned the hard way.

Trying to learn as I go and hate learning the hard way.

I'm really trying to get more into this hobby. I got 500 disposable plates, grafting tape and would like to pour roughly 50 to 100 total this week.

help and guidance plz

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Learning from cultivation knocks - human error
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tryptkaloids
Learner

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Geezed]

2
#29354534 - 09/24/25 07:25 PM (1 year, 20 days ago)

The simple solution is to not handle hot glass

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Gubar1
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Registered: 08/15/25
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]

3
#29354639 - 09/24/25 09:22 PM (1 year, 20 days ago)

Some LC testing. First time using agar.

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RoscoeReturns
We’ve outstayed our welcome

Registered: 02/13/18
Posts: 3,121
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Geezed]

1
#29354670 - 09/24/25 10:01 PM (1 year, 20 days ago)

Geezed said:

Geezed said:

Will these explode from thermal shock if I take them from PC @ 150 f and put them in a cooler temp at say consistent 120 f?

Just nervous after the quart jar 9ish years ago of LC exploded on me. Do to me not paying attention and went to go wash my hands in the sink next to my LC got hit with cold water. Lesson learned the hard way.

Trying to learn as I go and hate learning the hard way.

I'm really trying to get more into this hobby. I got 500 disposable plates, grafting tape and would like to pour roughly 50 to 100 total this week.

help and guidance plz

The answer is maybe? :shrug:

:shrug:

I don’t know that brand. I routinely take hot glass to a much cooler water bath with no problem, but I’m using Corning labware. I’ve heard too many horror stories from no name amazon glassware.

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Geezed
Blessed

Registered: 04/15/23
Posts: 74
Loc: Lost Lands
Last seen: 5 days, 7 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: RoscoeReturns]
#29354816 - 09/25/25 12:00 AM (1 year, 20 days ago)

Thanks.
Guess there's only one way to find out. I was gonna run it on my induction burner near flowhood. Think I'll keep it in the other room so I don't mess up the hood.

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Learning from cultivation knocks - human error
The Beeftube - getting the beef

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fahtster
Now With 33%More Faht

Registered: 06/17/06
Posts: 10,901

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Gubar1]
#29354845 - 09/25/25 12:41 AM (1 year, 20 days ago)

Gubar1 said:

Some LC testing. First time using agar.

Looks like some clean lc to my eyes

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Gilmour Hendrix
Village Idiot

Registered: 08/02/25
Posts: 1,689
Loc: Bacta-Mold HQ

Re: AGAR ENVY! (Anything and All things agar!)

2
#29354935 - 09/25/25 04:31 AM (1 year, 20 days ago)

Some of my favorite plates right now. 1&2 are x7x+ T1 from spore. And the last one is a Pan "TTBVI" T1 I am cleaning up from spore.

Thanks, Hippie!

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Headass

Registered: 07/24/24
Posts: 827
Loc: Texas

Last seen: 6 months, 19 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Gilmour Hendrix]

1
#29354948 - 09/25/25 05:51 AM (1 year, 20 days ago)

This is an update using TSA plates. These are after first transfer and did reveal contams hiding underneath. Using backlighting, those contams showed up very obvious. I transferred from these plates to regular LME plates

Reishi

Melmac

TSA is a bit expensive but I really like how clear the plates are and, for me, the contams showed very clearly for my old and inexperienced eyes.

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ihansterx4i
Stranger

Registered: 04/26/25
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Last seen: 7 hours, 47 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Headass]
#29357262 - 09/27/25 11:58 AM (1 year, 17 days ago)

First time getting ochra and it’s definitely very aggressive as I’ve read but is this normal for agar? This was the first transfer from spores.

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LotKid
Never.Trust.A.Prankster

Registered: 01/07/17
Posts: 8,883
Loc: Shakedown St.

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ihansterx4i]
#29357270 - 09/27/25 12:03 PM (1 year, 17 days ago)

So, okra is a fungus these day?

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AZ Starman2112
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Registered: 12/11/24
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: LotKid]
#29357362 - 09/27/25 01:03 PM (1 year, 17 days ago)

ihansterx4i said:

First time getting ochra and it’s definitely very aggressive as I’ve read but is this normal for agar? This was the first transfer from spores.

That does not look like any of the Ochra plates I’ve done. The last two pics, I’d try and take a very small clip of the outer edges and put it to new plates. The top two I’m not so sure about.

LotKid said:

So, okra is a fungus these day?

Got any of dem okra spores?
:lmafo:

:lmafo:

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ihansterx4i
Stranger

Registered: 04/26/25
Posts: 592
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]
#29357377 - 09/27/25 01:11 PM (1 year, 17 days ago)

Haha ok thanks, that’s what I thought.

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AZ Starman2112
Male Half of a Friendly Couple

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: ihansterx4i]
#29357390 - 09/27/25 01:24 PM (1 year, 17 days ago)

ihansterx4i said:

Haha ok thanks, that’s what I thought.

To give you an idea of what you are looking for, these are the last plates of Ochras I sent to grain. One was from a Plin off a germ plate, the other a T1 plate.

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ihansterx4i
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Registered: 04/26/25
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]

1
#29357623 - 09/27/25 05:33 PM (1 year, 17 days ago)

Beautiful plates. Wow ok thank you. How did you manage to cut the transfer in a circle like that?

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cronicr

Registered: 08/07/11
Posts: 65,498
Loc: Van Isle

Last seen: 2 days, 20 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ihansterx4i]

1
#29357632 - 09/27/25 05:44 PM (1 year, 17 days ago)

Leather punches work great

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rockyfungus
dirty

Registered: 03/01/21
Posts: 1,097
Loc: Front range
Last seen: 3 months, 8 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Sulfurshelfsean]

1
#29357679 - 09/27/25 06:21 PM (1 year, 17 days ago)

Sulfurshelfsean said:

Does this look okay? I'm trying to clean up some cultures I have that always seem to do good up until I fruit and get green mold out the ass. I took a couple pieces of the very edge and transferred. I have a feeling the fuzzy growth is a contam.

Hard to tell TBH, I'm pretty picky. Transfer and watch again!

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AZ Starman2112
Male Half of a Friendly Couple

Registered: 12/11/24
Posts: 2,640
Loc: A Steamy Bedroom With The Cool...
Last seen: 2 days, 19 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: cronicr]
#29357786 - 09/27/25 07:44 PM (1 year, 17 days ago)

ihansterx4i said:

Beautiful plates. Wow ok thank you. How did you manage to cut the transfer in a circle like that?

cronicr said:

Leather punches work great

:whathesaid:

8mm metal leather punch, which some may say is to big.

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Tropical
Here

Registered: 07/21/24
Posts: 821

Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]
#29357861 - 09/27/25 08:47 PM (1 year, 17 days ago)

How to know if it s mold / contam or some fluffy myc ?

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wow

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